Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR79_RS06290 Genome accession   NZ_CP103824
Coordinates   1334252..1335637 (+) Length   461 a.a.
NCBI ID   WP_279454528.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3524     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1329252..1340637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR79_RS06265 (NYR79_06265) greB 1329321..1329809 (-) 489 WP_005611555.1 transcription elongation factor GreB -
  NYR79_RS06270 (NYR79_06270) - 1329819..1330754 (-) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR79_RS06275 (NYR79_06275) pepB 1330882..1332168 (-) 1287 WP_279455506.1 aminopeptidase PepB -
  NYR79_RS06280 (NYR79_06280) radA 1332234..1333613 (-) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR79_RS06285 (NYR79_06285) pilA 1333794..1334231 (+) 438 WP_279454527.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR79_RS06290 (NYR79_06290) pilB 1334252..1335637 (+) 1386 WP_279454528.1 GspE/PulE family protein Machinery gene
  NYR79_RS06295 (NYR79_06295) pilC 1335630..1336823 (+) 1194 WP_279454529.1 type II secretion system F family protein Machinery gene
  NYR79_RS06300 (NYR79_06300) - 1336823..1337497 (+) 675 WP_039197142.1 fimbrial protein -
  NYR79_RS06305 (NYR79_06305) coaE 1337519..1338157 (+) 639 WP_279454029.1 dephospho-CoA kinase -
  NYR79_RS06310 (NYR79_06310) yacG 1338139..1338327 (+) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR79_RS06315 (NYR79_06315) holA 1338404..1339429 (-) 1026 WP_279454530.1 DNA polymerase III subunit delta -
  NYR79_RS06320 (NYR79_06320) lptE 1339537..1340031 (-) 495 WP_278225775.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52515.11 Da        Isoelectric Point: 5.3508

>NTDB_id=725601 NYR79_RS06290 WP_279454528.1 1334252..1335637(+) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3524]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDVQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLTALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDRESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGVKEYEIKNSLQLIIAQRLLRKSCPQCGGKGC
DNCYHGYKGRTGIYQLLTKQGKFFERSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725601 NYR79_RS06290 WP_279454528.1 1334252..1335637(+) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3524]
ATGCAGTATTCGGTTTGTGATATGAATAGCCAGCAGGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGTTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCAGATAA
TTTAGAACTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGTGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAGCAATTATTACTTACAGCGTTACACCAGCCACAAGGGTTGATTCTGGTTACGGGACCAACAGGCAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACTGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTAAATCGTGCAATTCAATTAGACTTTAGCCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCGGGAAAGTGCCGAAATTGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAACGCTTATTACAATTGGGAGTTAAAGAGTACG
AAATTAAGAATTCTCTGCAACTGATCATTGCACAGCGTTTATTACGTAAAAGTTGTCCGCAATGTGGCGGAAAAGGATGT
GATAACTGTTATCACGGTTATAAAGGGCGAACGGGAATTTATCAGTTATTGACGAAGCAAGGTAAATTTTTTGAAAGATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.271

99.349

0.718
  pilB Haemophilus influenzae Rd KW20

56.763

97.831

0.555
  pilB Haemophilus influenzae 86-028NP

56.098

97.831

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

40.748

100

0.425
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.423
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.423
  pilB Vibrio cholerae strain A1552

38.956

100

0.421
  pilB Acinetobacter baumannii D1279779

38.508

100

0.414
  pilB Acinetobacter baylyi ADP1

37.773

100

0.412