Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR79_RS06285 Genome accession   NZ_CP103824
Coordinates   1333794..1334231 (+) Length   145 a.a.
NCBI ID   WP_279454527.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3524     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1328794..1339231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR79_RS06265 (NYR79_06265) greB 1329321..1329809 (-) 489 WP_005611555.1 transcription elongation factor GreB -
  NYR79_RS06270 (NYR79_06270) - 1329819..1330754 (-) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR79_RS06275 (NYR79_06275) pepB 1330882..1332168 (-) 1287 WP_279455506.1 aminopeptidase PepB -
  NYR79_RS06280 (NYR79_06280) radA 1332234..1333613 (-) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR79_RS06285 (NYR79_06285) pilA 1333794..1334231 (+) 438 WP_279454527.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR79_RS06290 (NYR79_06290) pilB 1334252..1335637 (+) 1386 WP_279454528.1 GspE/PulE family protein Machinery gene
  NYR79_RS06295 (NYR79_06295) pilC 1335630..1336823 (+) 1194 WP_279454529.1 type II secretion system F family protein Machinery gene
  NYR79_RS06300 (NYR79_06300) - 1336823..1337497 (+) 675 WP_039197142.1 fimbrial protein -
  NYR79_RS06305 (NYR79_06305) coaE 1337519..1338157 (+) 639 WP_279454029.1 dephospho-CoA kinase -
  NYR79_RS06310 (NYR79_06310) yacG 1338139..1338327 (+) 189 WP_005623139.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15459.77 Da        Isoelectric Point: 8.0828

>NTDB_id=725600 NYR79_RS06285 WP_279454527.1 1333794..1334231(+) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3524]
MKLMIKPIQRAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGETKSCSSGTNGIKN
GDDLKNSKYLDSVSVTAGTITVNGKGSVAGYGYTLTPDYSSKTLTWKTTCSGEDKSLFPAAFCSE

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725600 NYR79_RS06285 WP_279454527.1 1333794..1334231(+) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3524]
ATGAAACTCATGATCAAGCCTATACAACGGGCATTTACACTGATTGAATTAATGATTGTAATTGCAATCATCGCAATTTT
GGCAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCAGCATTATCTGAGCTTTTGGCGGCATCTTCGGCGT
ATAAAACGGATGTGGAAATTTGTATTTATAACACCGGCGAGACAAAAAGTTGTTCTAGTGGAACGAATGGCATTAAAAAT
GGCGATGATCTTAAAAATTCTAAGTATTTAGATTCAGTCTCTGTAACTGCGGGTACTATAACCGTGAATGGCAAGGGCAG
CGTAGCGGGATATGGTTATACACTTACACCAGATTATTCAAGTAAAACACTGACGTGGAAAACAACCTGTTCAGGAGAGG
ATAAAAGTTTATTCCCCGCAGCATTTTGCTCGGAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

62.069

100

0.621
  pilA Haemophilus influenzae Rd KW20

57.534

100

0.579
  pilA Haemophilus influenzae 86-028NP

56.849

100

0.572
  comP Acinetobacter baylyi ADP1

41.135

97.241

0.4
  pilA Vibrio campbellii strain DS40M4

37.589

97.241

0.366