Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR75_RS04835 Genome accession   NZ_CP103820
Coordinates   1052046..1053431 (-) Length   461 a.a.
NCBI ID   WP_279472248.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3820     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1047046..1058431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR75_RS04805 (NYR75_04810) lptE 1047652..1048146 (+) 495 WP_278225775.1 LPS assembly lipoprotein LptE -
  NYR75_RS04810 (NYR75_04815) holA 1048254..1049279 (+) 1026 WP_279454530.1 DNA polymerase III subunit delta -
  NYR75_RS04815 (NYR75_04820) yacG 1049356..1049544 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR75_RS04820 (NYR75_04825) coaE 1049526..1050164 (-) 639 WP_279454029.1 dephospho-CoA kinase -
  NYR75_RS04825 (NYR75_04830) - 1050186..1050860 (-) 675 WP_039197142.1 fimbrial protein -
  NYR75_RS04830 (NYR75_04835) pilC 1050860..1052053 (-) 1194 WP_279454529.1 type II secretion system F family protein Machinery gene
  NYR75_RS04835 (NYR75_04840) pilB 1052046..1053431 (-) 1386 WP_279472248.1 GspE/PulE family protein Machinery gene
  NYR75_RS04840 (NYR75_04845) pilA 1053452..1053889 (-) 438 WP_005623151.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR75_RS04845 (NYR75_04850) radA 1054069..1055448 (+) 1380 WP_014991736.1 DNA repair protein RadA Machinery gene
  NYR75_RS04850 (NYR75_04855) pepB 1055514..1056800 (+) 1287 WP_279472309.1 aminopeptidase PepB -
  NYR75_RS04855 (NYR75_04860) - 1056928..1057863 (+) 936 WP_279472249.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR75_RS04860 (NYR75_04865) greB 1057873..1058361 (+) 489 WP_279472250.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52473.03 Da        Isoelectric Point: 5.3508

>NTDB_id=725514 NYR75_RS04835 WP_279472248.1 1052046..1053431(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3820]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLTALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDRESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGVKEYEIKNSLQLIIAQRLLRKSCPQCGGKGC
DNCYHGYKGRTGIYQLLTKQGKFFERSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725514 NYR75_RS04835 WP_279472248.1 1052046..1053431(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3820]
ATGCAGTATTCGGTTTGTGATATGAATAGCCAGCAGGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGTTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCAGATAA
TTTAGAACTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAGCAATTATTACTTACAGCGTTACACCAGCCACAAGGGCTGATTCTGGTTACGGGACCAACAGGCAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACTGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTAAATCGTGCAATTCAATTAGACTTTAGCCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCGGGAAAGTGCCGAAATTGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAACGCTTATTACAATTGGGAGTTAAAGAGTACG
AAATTAAGAATTCTCTGCAACTGATCATTGCACAGCGTTTATTACGTAAAAGTTGTCCGCAATGTGGCGGAAAAGGATGT
GATAACTGTTATCACGGTTATAAAGGGCGAACGGGAATTTATCAGTTATTGACGAAGCAAGGTAAATTTTTTGAAAGATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.489

99.349

0.72
  pilB Haemophilus influenzae Rd KW20

56.984

97.831

0.557
  pilB Haemophilus influenzae 86-028NP

56.319

97.831

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

40.956

100

0.427
  pilB Vibrio campbellii strain DS40M4

40.412

100

0.425
  pilB Legionella pneumophila strain ERS1305867

39.837

100

0.425
  pilB Vibrio cholerae strain A1552

39.157

100

0.423
  pilB Acinetobacter baumannii D1279779

38.71

100

0.416
  pilB Acinetobacter baylyi ADP1

37.972

100

0.414