Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR75_RS04840 Genome accession   NZ_CP103820
Coordinates   1053452..1053889 (-) Length   145 a.a.
NCBI ID   WP_005623151.1    Uniprot ID   E8KHR2
Organism   Actinobacillus equuli subsp. haemolyticus strain 3820     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1048452..1058889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR75_RS04815 (NYR75_04820) yacG 1049356..1049544 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR75_RS04820 (NYR75_04825) coaE 1049526..1050164 (-) 639 WP_279454029.1 dephospho-CoA kinase -
  NYR75_RS04825 (NYR75_04830) - 1050186..1050860 (-) 675 WP_039197142.1 fimbrial protein -
  NYR75_RS04830 (NYR75_04835) pilC 1050860..1052053 (-) 1194 WP_279454529.1 type II secretion system F family protein Machinery gene
  NYR75_RS04835 (NYR75_04840) pilB 1052046..1053431 (-) 1386 WP_279472248.1 GspE/PulE family protein Machinery gene
  NYR75_RS04840 (NYR75_04845) pilA 1053452..1053889 (-) 438 WP_005623151.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR75_RS04845 (NYR75_04850) radA 1054069..1055448 (+) 1380 WP_014991736.1 DNA repair protein RadA Machinery gene
  NYR75_RS04850 (NYR75_04855) pepB 1055514..1056800 (+) 1287 WP_279472309.1 aminopeptidase PepB -
  NYR75_RS04855 (NYR75_04860) - 1056928..1057863 (+) 936 WP_279472249.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR75_RS04860 (NYR75_04865) greB 1057873..1058361 (+) 489 WP_279472250.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15335.53 Da        Isoelectric Point: 7.6225

>NTDB_id=725515 NYR75_RS04840 WP_005623151.1 1053452..1053889(-) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3820]
MKHIIKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGATNSCSSGANGIKN
GDDLKNSKYLDSVSVTAGTITVNGKGSVAGYGYTLTPDYSSKTLTWKTTCSGEDKSLFPAAFCSE

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725515 NYR75_RS04840 WP_005623151.1 1053452..1053889(-) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3820]
ATGAAACACATAATTAAACCGATACAACAGGCATTTACCCTGATTGAATTAATGATTGTGATTGCAATCATTGCAATTTT
AGCAACTGTGGCGATTCCTTCTTATAACAATTACACCAAAAAAGCGGCATTATCCGAACTGTTAGCGGCATCTTCTGCAT
ATAAAACGGATGTAGAAATTTGTATTTATAACACTGGAGCTACCAATAGCTGTTCTAGTGGAGCAAATGGCATTAAGAAT
GGCGATGATCTTAAAAATTCTAAGTATTTAGATTCAGTCTCTGTAACTGCGGGTACTATAACCGTGAATGGCAAGGGCAG
CGTAGCGGGATATGGTTATACACTTACACCAGATTATTCAAGTAAAACACTGACGTGGAAAACAACCTGTTCAGGAGAGG
ATAAAAGTTTATTCCCCGCAGCATTTTGCTCGGAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E8KHR2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

60.959

100

0.614
  pilA Haemophilus influenzae Rd KW20

56.738

97.241

0.552
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  comP Acinetobacter baylyi ADP1

41.844

97.241

0.407