Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR71_RS04720 Genome accession   NZ_CP103817
Coordinates   1077570..1078955 (-) Length   461 a.a.
NCBI ID   WP_279440208.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072570..1083955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR71_RS04690 (NYR71_04690) lptE 1073176..1073670 (+) 495 WP_278225775.1 LPS assembly lipoprotein LptE -
  NYR71_RS04695 (NYR71_04695) holA 1073778..1074803 (+) 1026 WP_279440204.1 DNA polymerase III subunit delta -
  NYR71_RS04700 (NYR71_04700) yacG 1074880..1075068 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR71_RS04705 (NYR71_04705) coaE 1075050..1075688 (-) 639 WP_279440205.1 dephospho-CoA kinase -
  NYR71_RS04710 (NYR71_04710) - 1075710..1076384 (-) 675 WP_279440206.1 prepilin peptidase -
  NYR71_RS04715 (NYR71_04715) pilC 1076384..1077577 (-) 1194 WP_279440207.1 type II secretion system F family protein Machinery gene
  NYR71_RS04720 (NYR71_04720) pilB 1077570..1078955 (-) 1386 WP_279440208.1 GspE/PulE family protein Machinery gene
  NYR71_RS04725 (NYR71_04725) pilA 1078976..1079413 (-) 438 WP_279439097.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR71_RS04730 (NYR71_04730) radA 1079594..1080973 (+) 1380 WP_014991736.1 DNA repair protein RadA Machinery gene
  NYR71_RS04735 (NYR71_04735) pepB 1081039..1082322 (+) 1284 WP_279440258.1 aminopeptidase PepB -
  NYR71_RS04740 (NYR71_04740) - 1082450..1083385 (+) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR71_RS04745 (NYR71_04745) greB 1083395..1083883 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52459.02 Da        Isoelectric Point: 5.3508

>NTDB_id=725440 NYR71_RS04720 WP_279440208.1 1077570..1078955(-) (pilB) [Actinobacillus equuli subsp. equuli strain 4005]
MQYSVCDMNSQQLFEISDSLWQKNCDEKHVLLRYLAMPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSVYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLQIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLAALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725440 NYR71_RS04720 WP_279440208.1 1077570..1078955(-) (pilB) [Actinobacillus equuli subsp. equuli strain 4005]
ATGCAGTATTCGGTTTGTGATATGAACAGCCAGCAGCTATTTGAAATTTCAGACTCGTTGTGGCAAAAAAATTGTGATGA
AAAACATGTTTTATTACGTTATTTGGCTATGCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTGAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTATTTACTTAATCAACTTTCGCCAGAACAACAATCCGTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAACTACTCAATCAAAATGACCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGACATTCACTTTGAACCGCAAAAAGAGAAGCTACAGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTAGATATTAGTGAGACACGTTTACC
ACAAGACGGCCAATTTAATTTTAAGACGGTAACGGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAACAATTACTACTGGCAGCACTACATCAGCCACAAGGGCTGATTCTGGTGACAGGACCTACGGGTAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGTGCTATTCAATTGGATTTTAGTCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCACCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCAAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.707

99.349

0.722
  pilB Haemophilus influenzae Rd KW20

57.428

97.831

0.562
  pilB Haemophilus influenzae 86-028NP

56.984

97.831

0.557
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

40.333

100

0.421
  pilB Legionella pneumophila strain ERS1305867

39.431

100

0.421
  pilB Vibrio cholerae strain A1552

39.627

100

0.414
  pilB Acinetobacter baylyi ADP1

37.827

100

0.408
  pilB Acinetobacter baumannii D1279779

38.683

100

0.408