Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR71_RS04725 Genome accession   NZ_CP103817
Coordinates   1078976..1079413 (-) Length   145 a.a.
NCBI ID   WP_279439097.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1073976..1084413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR71_RS04700 (NYR71_04700) yacG 1074880..1075068 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR71_RS04705 (NYR71_04705) coaE 1075050..1075688 (-) 639 WP_279440205.1 dephospho-CoA kinase -
  NYR71_RS04710 (NYR71_04710) - 1075710..1076384 (-) 675 WP_279440206.1 prepilin peptidase -
  NYR71_RS04715 (NYR71_04715) pilC 1076384..1077577 (-) 1194 WP_279440207.1 type II secretion system F family protein Machinery gene
  NYR71_RS04720 (NYR71_04720) pilB 1077570..1078955 (-) 1386 WP_279440208.1 GspE/PulE family protein Machinery gene
  NYR71_RS04725 (NYR71_04725) pilA 1078976..1079413 (-) 438 WP_279439097.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR71_RS04730 (NYR71_04730) radA 1079594..1080973 (+) 1380 WP_014991736.1 DNA repair protein RadA Machinery gene
  NYR71_RS04735 (NYR71_04735) pepB 1081039..1082322 (+) 1284 WP_279440258.1 aminopeptidase PepB -
  NYR71_RS04740 (NYR71_04740) - 1082450..1083385 (+) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR71_RS04745 (NYR71_04745) greB 1083395..1083883 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15499.86 Da        Isoelectric Point: 8.0828

>NTDB_id=725441 NYR71_RS04725 WP_279439097.1 1078976..1079413(-) (pilA) [Actinobacillus equuli subsp. equuli strain 4005]
MKFVFKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGEVKNCSSGTNGIKN
GNDIKNSKYLASISVASGVITVKGKGSVDGYGYTLTPDYSNKTLTWETACLGEDTSLFPAAFCSK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725441 NYR71_RS04725 WP_279439097.1 1078976..1079413(-) (pilA) [Actinobacillus equuli subsp. equuli strain 4005]
ATGAAATTCGTATTCAAGCCTATACAGCAGGCATTTACTCTGATTGAATTAATGATTGTAATTGCAATCATTGCTATTTT
GGCAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCGGCATTATCCGAACTGTTAGCGGCATCTTCCGCTT
ATAAAACAGATGTGGAAATTTGTATTTATAACACGGGAGAGGTAAAAAACTGTTCTAGCGGAACGAATGGCATCAAAAAT
GGTAACGATATCAAAAACTCTAAGTATTTAGCCTCTATTTCTGTAGCTTCCGGTGTAATAACTGTGAAGGGGAAAGGGAG
TGTAGATGGGTATGGATATACGCTTACACCGGATTATTCAAATAAAACGCTAACTTGGGAAACGGCTTGTTTAGGCGAGG
ATACGAGTCTATTCCCTGCCGCGTTTTGTTCGAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

65.517

100

0.655
  pilA Haemophilus influenzae Rd KW20

56.738

97.241

0.552
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  pilA Vibrio campbellii strain DS40M4

37.589

97.241

0.366