Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR70_RS04710 Genome accession   NZ_CP103816
Coordinates   1066943..1068328 (-) Length   461 a.a.
NCBI ID   WP_279437884.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4026     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1061943..1073328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR70_RS04680 (NYR70_04680) lptE 1062549..1063043 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  NYR70_RS04685 (NYR70_04685) holA 1063151..1064176 (+) 1026 WP_279477758.1 DNA polymerase III subunit delta -
  NYR70_RS04690 (NYR70_04690) yacG 1064253..1064441 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR70_RS04695 (NYR70_04695) coaE 1064423..1065061 (-) 639 WP_279477760.1 dephospho-CoA kinase -
  NYR70_RS04700 (NYR70_04700) - 1065083..1065757 (-) 675 WP_275216987.1 prepilin peptidase -
  NYR70_RS04705 (NYR70_04705) pilC 1065757..1066950 (-) 1194 WP_278225778.1 type II secretion system F family protein Machinery gene
  NYR70_RS04710 (NYR70_04710) pilB 1066943..1068328 (-) 1386 WP_279437884.1 GspE/PulE family protein Machinery gene
  NYR70_RS04715 (NYR70_04715) pilA 1068349..1068786 (-) 438 WP_279477765.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR70_RS04720 (NYR70_04720) radA 1068967..1070346 (+) 1380 WP_279477767.1 DNA repair protein RadA Machinery gene
  NYR70_RS04725 (NYR70_04725) pepB 1070412..1071698 (+) 1287 WP_279477878.1 aminopeptidase PepB -
  NYR70_RS04730 (NYR70_04730) - 1071826..1072761 (+) 936 WP_279477769.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR70_RS04735 (NYR70_04735) greB 1072771..1073259 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52440.05 Da        Isoelectric Point: 5.3508

>NTDB_id=725413 NYR70_RS04710 WP_279437884.1 1066943..1068328(-) (pilB) [Actinobacillus equuli subsp. equuli strain 4026]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLAALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725413 NYR70_RS04710 WP_279437884.1 1066943..1068328(-) (pilB) [Actinobacillus equuli subsp. equuli strain 4026]
ATGCAGTATTCAGTTTGTGATATGAATAGTCAGCAAGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAACTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCATATCTATAAAAGC
TTATCGTTTAGTTTAGCCAATCGTATTATCACAAGGCTTAAACTCTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAACAATTACTACTGGCAGCACTACATCAGCCACAAGGGCTGATTCTGGTGACAGGACCTACGGGTAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGTGCTATTCAATTGGATTTTAGTCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.926

99.349

0.725
  pilB Haemophilus influenzae Rd KW20

57.206

97.831

0.56
  pilB Haemophilus influenzae 86-028NP

56.541

97.831

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

40.541

100

0.423
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.423
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.423
  pilB Vibrio cholerae strain A1552

38.956

100

0.421
  pilB Acinetobacter baumannii D1279779

38.508

100

0.414
  pilB Acinetobacter baylyi ADP1

37.773

100

0.412