Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR70_RS04715 Genome accession   NZ_CP103816
Coordinates   1068349..1068786 (-) Length   145 a.a.
NCBI ID   WP_279477765.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4026     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1063349..1073786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR70_RS04690 (NYR70_04690) yacG 1064253..1064441 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR70_RS04695 (NYR70_04695) coaE 1064423..1065061 (-) 639 WP_279477760.1 dephospho-CoA kinase -
  NYR70_RS04700 (NYR70_04700) - 1065083..1065757 (-) 675 WP_275216987.1 prepilin peptidase -
  NYR70_RS04705 (NYR70_04705) pilC 1065757..1066950 (-) 1194 WP_278225778.1 type II secretion system F family protein Machinery gene
  NYR70_RS04710 (NYR70_04710) pilB 1066943..1068328 (-) 1386 WP_279437884.1 GspE/PulE family protein Machinery gene
  NYR70_RS04715 (NYR70_04715) pilA 1068349..1068786 (-) 438 WP_279477765.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR70_RS04720 (NYR70_04720) radA 1068967..1070346 (+) 1380 WP_279477767.1 DNA repair protein RadA Machinery gene
  NYR70_RS04725 (NYR70_04725) pepB 1070412..1071698 (+) 1287 WP_279477878.1 aminopeptidase PepB -
  NYR70_RS04730 (NYR70_04730) - 1071826..1072761 (+) 936 WP_279477769.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR70_RS04735 (NYR70_04735) greB 1072771..1073259 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15520.88 Da        Isoelectric Point: 7.5775

>NTDB_id=725414 NYR70_RS04715 WP_279477765.1 1068349..1068786(-) (pilA) [Actinobacillus equuli subsp. equuli strain 4026]
MKLVFKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGEVKNCSSGTNGIKN
GNDIKNAKYLDSISVASGVITVKGKGNVDGYGYTLTPDYSNKTLTWETACLGEDTSLFPAAFCSK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725414 NYR70_RS04715 WP_279477765.1 1068349..1068786(-) (pilA) [Actinobacillus equuli subsp. equuli strain 4026]
ATGAAACTCGTATTCAAGCCTATACAGCAGGCATTTACCTTGATTGAATTAATGATTGTAATTGCAATCATTGCTATTTT
GGCAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCGGCATTATCCGAACTGTTGGCGGCATCTTCCGCTT
ATAAAACAGATGTGGAAATTTGTATTTATAACACGGGAGAGGTAAAAAACTGTTCTAGCGGAACGAATGGCATCAAAAAT
GGTAACGATATCAAAAACGCTAAGTATTTAGATTCTATTTCTGTAGCTTCCGGTGTAATAACGGTGAAGGGGAAAGGGAA
TGTAGATGGGTATGGCTATACGCTTACACCGGATTATTCAAATAAAACGCTAACTTGGGAGACGGCTTGTTTAGGCGAGG
ATACTAGTCTATTCCCTGCCGCGTTTTGTTCGAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.897

100

0.669
  pilA Haemophilus influenzae 86-028NP

56.849

100

0.572
  pilA Haemophilus influenzae Rd KW20

56.849

100

0.572
  pilA Vibrio campbellii strain DS40M4

37.589

97.241

0.366