Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR68_RS05165 Genome accession   NZ_CP103814
Coordinates   1123041..1124426 (-) Length   461 a.a.
NCBI ID   WP_279437884.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 4091     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1118041..1129426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR68_RS05130 (NYR68_05135) lptE 1118647..1119141 (+) 495 WP_278225775.1 LPS assembly lipoprotein LptE -
  NYR68_RS05135 (NYR68_05140) - 1119146..1119292 (-) 147 WP_278225056.1 hypothetical protein -
  NYR68_RS05140 (NYR68_05145) holA 1119249..1120274 (+) 1026 WP_279622773.1 DNA polymerase III subunit delta -
  NYR68_RS05145 (NYR68_05150) yacG 1120351..1120539 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR68_RS05150 (NYR68_05155) coaE 1120521..1121159 (-) 639 WP_279441162.1 dephospho-CoA kinase -
  NYR68_RS05155 (NYR68_05160) - 1121181..1121855 (-) 675 WP_039197142.1 fimbrial protein -
  NYR68_RS05160 (NYR68_05165) pilC 1121855..1123048 (-) 1194 WP_279454529.1 type II secretion system F family protein Machinery gene
  NYR68_RS05165 (NYR68_05170) pilB 1123041..1124426 (-) 1386 WP_279437884.1 GspE/PulE family protein Machinery gene
  NYR68_RS05170 (NYR68_05175) pilA 1124447..1124884 (-) 438 WP_279622774.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR68_RS05175 (NYR68_05180) radA 1125065..1126444 (+) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR68_RS05180 (NYR68_05185) pepB 1126510..1127796 (+) 1287 WP_279622822.1 aminopeptidase PepB -
  NYR68_RS05185 (NYR68_05190) - 1127924..1128859 (+) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR68_RS05190 (NYR68_05195) greB 1128869..1129357 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52440.05 Da        Isoelectric Point: 5.3508

>NTDB_id=725359 NYR68_RS05165 WP_279437884.1 1123041..1124426(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 4091]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLAALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725359 NYR68_RS05165 WP_279437884.1 1123041..1124426(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 4091]
ATGCAGTATTCAGTTTGTGATATGAATAGTCAGCAAGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCAGATAA
TTTAGAACTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCATATCTATAAAAGC
TTATCGTTTAGTTTAGCCAATCGTATTATCACAAGGCTTAAACTCTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAACAATTACTACTGGCAGCACTACATCAGCCACAAGGGCTAATTCTGGTGACAGGACCTACGGGTAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGTGCTATTCAATTGGATTTTAGTCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.926

99.349

0.725
  pilB Haemophilus influenzae Rd KW20

57.206

97.831

0.56
  pilB Haemophilus influenzae 86-028NP

56.541

97.831

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

40.541

100

0.423
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.423
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.423
  pilB Vibrio cholerae strain A1552

38.956

100

0.421
  pilB Acinetobacter baumannii D1279779

38.508

100

0.414
  pilB Acinetobacter baylyi ADP1

37.773

100

0.412