Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR61_RS04910 Genome accession   NZ_CP103808
Coordinates   1093676..1095070 (-) Length   464 a.a.
NCBI ID   WP_279469456.1    Uniprot ID   -
Organism   Actinobacillus genomosp. 1 strain CCUG22231     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1088676..1100070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR61_RS04880 (NYR61_04880) lptE 1089298..1089792 (+) 495 WP_279469451.1 LPS assembly lipoprotein LptE -
  NYR61_RS04885 (NYR61_04885) holA 1089894..1090919 (+) 1026 WP_279469452.1 DNA polymerase III subunit delta -
  NYR61_RS04890 (NYR61_04890) yacG 1090996..1091184 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR61_RS04895 (NYR61_04895) coaE 1091120..1091803 (-) 684 WP_279469453.1 dephospho-CoA kinase -
  NYR61_RS04900 (NYR61_04900) - 1091825..1092469 (-) 645 WP_347710238.1 prepilin peptidase -
  NYR61_RS04905 (NYR61_04905) - 1092490..1093692 (-) 1203 WP_279469455.1 type II secretion system F family protein -
  NYR61_RS04910 (NYR61_04910) pilB 1093676..1095070 (-) 1395 WP_279469456.1 GspE/PulE family protein Machinery gene
  NYR61_RS04915 (NYR61_04915) pilA 1095088..1095534 (-) 447 WP_279458428.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR61_RS04920 (NYR61_04920) radA 1095714..1097093 (+) 1380 WP_279469457.1 DNA repair protein RadA Machinery gene
  NYR61_RS04925 (NYR61_04925) - 1097136..1097369 (-) 234 WP_237593621.1 YgjV family protein -
  NYR61_RS04930 (NYR61_04930) folA 1097499..1097987 (-) 489 WP_279469458.1 type 3 dihydrofolate reductase -
  NYR61_RS04935 (NYR61_04935) proB 1098074..1099165 (+) 1092 WP_279458431.1 glutamate 5-kinase -
  NYR61_RS04940 (NYR61_04940) - 1099181..1099957 (+) 777 WP_279469459.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52817.46 Da        Isoelectric Point: 5.4643

>NTDB_id=725197 NYR61_RS04910 WP_279469456.1 1093676..1095070(-) (pilB) [Actinobacillus genomosp. 1 strain CCUG22231]
MQYSVCDINSRQVLRISDERWQKNVEEGHILLRYLAVPIQEDEQILWLAIDDMNNLSACEIFAFIAHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYEETELHYQSLEQTEYLDFNDPIIQVLDSLFKYSLKHNVSDIHLEPQKDNMLIRLRIDGVLHHYKS
LSLQLANRILSRIKLLAKLDISESRLPQDGQFSFKTALSETLDFRVSSLPTNWGEKVVLRLQKNKPTHFDFISLGFLAEQ
QEKLLQALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELDGIIQTQVNPVIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVIAQRLIRKLCTQCGGTGC
KHCYHGYNGRIGVYQLFSKNGKIFEKSTACLDFDNLYQSAQQRLNAGVTSLEEINRVLGNVESI

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=725197 NYR61_RS04910 WP_279469456.1 1093676..1095070(-) (pilB) [Actinobacillus genomosp. 1 strain CCUG22231]
ATGCAGTATTCGGTATGTGACATAAACAGTAGGCAAGTATTGCGGATCTCCGATGAACGATGGCAAAAAAATGTTGAGGA
AGGTCATATTCTATTACGTTATCTTGCCGTACCGATTCAGGAAGATGAGCAAATCTTGTGGCTTGCAATCGACGATATGA
ATAATCTCAGTGCTTGCGAAATTTTTGCTTTTATTGCTCATAAGCGAATTGAACCGGTATTGGTTTCCTCCGAAGAATTG
AAATATCTACTTGGACAATTAATGCCGGAACAACAGTCCGTATATGAAGAAACCGAACTACATTATCAGTCTTTAGAACA
AACGGAATATTTGGATTTTAATGATCCTATTATTCAAGTGTTAGATAGCCTGTTTAAATACAGTTTAAAGCATAATGTAT
CGGATATTCATTTAGAACCGCAGAAAGACAATATGCTGATTCGATTACGTATTGACGGTGTGTTACATCACTATAAATCA
CTTTCTCTTCAGCTTGCCAATCGTATTCTTTCTCGAATTAAATTATTAGCGAAACTCGATATTAGCGAATCTCGTCTTCC
GCAAGACGGGCAATTTAGCTTTAAAACCGCATTATCCGAAACATTGGATTTTCGCGTTTCAAGTTTACCGACTAATTGGG
GAGAGAAAGTAGTTTTACGTTTACAAAAAAATAAGCCTACGCATTTCGATTTTATTTCCTTAGGCTTTTTAGCGGAACAG
CAAGAAAAACTATTACAGGCACTGCGACAACCCCAAGGGCTAATACTGGTAACAGGGCCGACGGGCAGCGGTAAGAGCAT
TACGTTATACAGCGCATTAGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCGGAAGATCCTATTGAAATCGAAC
TTGACGGAATTATTCAAACGCAAGTAAATCCGGTGATAGATTTAGGTTTTAGTCGGTTATTGAGGACGTTTTTACGTCAA
GATCCGGATGTGATTATGTTAGGGGAGATTCGTGATCAAGAAAGTGCCGAAATAGCGTTGAGTGCGGCGCAAACGGGGCA
TTTGGTTCTCTCTACTTTGCACACTAATGATGCGCCTTCGGCTATTGAACGTTTATTACAACTCGGTATAAAAGAATATG
AAATCAATAATTCGTTATTACTTGTTATAGCGCAACGTTTAATACGTAAATTATGTACTCAATGCGGCGGGACAGGATGT
AAACATTGTTATCACGGTTACAACGGGAGAATCGGCGTTTATCAGCTGTTTTCAAAAAATGGCAAAATTTTTGAAAAATC
GACCGCTTGTCTGGATTTTGACAATTTGTATCAAAGTGCGCAACAGAGACTAAATGCCGGCGTGACTTCATTGGAAGAAA
TTAACAGAGTATTAGGTAATGTCGAAAGTATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

69.651

98.707

0.688
  pilB Haemophilus influenzae Rd KW20

57.65

97.198

0.56
  pilB Haemophilus influenzae 86-028NP

56.984

97.198

0.554
  pilB Acinetobacter baumannii D1279779

39.712

100

0.416
  pilB Acinetobacter baylyi ADP1

38.683

100

0.405
  pilB Vibrio campbellii strain DS40M4

39.04

100

0.403
  pilB Vibrio parahaemolyticus RIMD 2210633

38.831

100

0.401
  pilB Vibrio cholerae strain A1552

38.669

100

0.401
  pilB Legionella pneumophila strain ERS1305867

37.628

100

0.397
  pilF Neisseria gonorrhoeae MS11

35.443

100

0.362