Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR61_RS04915 Genome accession   NZ_CP103808
Coordinates   1095088..1095534 (-) Length   148 a.a.
NCBI ID   WP_279458428.1    Uniprot ID   -
Organism   Actinobacillus genomosp. 1 strain CCUG22231     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1090088..1100534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR61_RS04890 (NYR61_04890) yacG 1090996..1091184 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR61_RS04895 (NYR61_04895) coaE 1091120..1091803 (-) 684 WP_279469453.1 dephospho-CoA kinase -
  NYR61_RS04900 (NYR61_04900) - 1091825..1092469 (-) 645 WP_347710238.1 prepilin peptidase -
  NYR61_RS04905 (NYR61_04905) - 1092490..1093692 (-) 1203 WP_279469455.1 type II secretion system F family protein -
  NYR61_RS04910 (NYR61_04910) pilB 1093676..1095070 (-) 1395 WP_279469456.1 GspE/PulE family protein Machinery gene
  NYR61_RS04915 (NYR61_04915) pilA 1095088..1095534 (-) 447 WP_279458428.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR61_RS04920 (NYR61_04920) radA 1095714..1097093 (+) 1380 WP_279469457.1 DNA repair protein RadA Machinery gene
  NYR61_RS04925 (NYR61_04925) - 1097136..1097369 (-) 234 WP_237593621.1 YgjV family protein -
  NYR61_RS04930 (NYR61_04930) folA 1097499..1097987 (-) 489 WP_279469458.1 type 3 dihydrofolate reductase -
  NYR61_RS04935 (NYR61_04935) proB 1098074..1099165 (+) 1092 WP_279458431.1 glutamate 5-kinase -
  NYR61_RS04940 (NYR61_04940) - 1099181..1099957 (+) 777 WP_279469459.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16022.42 Da        Isoelectric Point: 8.0852

>NTDB_id=725198 NYR61_RS04915 WP_279458428.1 1095088..1095534(-) (pilA) [Actinobacillus genomosp. 1 strain CCUG22231]
MQKLNLIRPLTKAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLSASASYKTDVEICMYNTGEAKNCSSGQNGV
SKITELKRAKYLNTITVESGTITVTGKGNLQEYGYTMTPNYNGNTISWETKCNGADLSLFPANFCAAN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=725198 NYR61_RS04915 WP_279458428.1 1095088..1095534(-) (pilA) [Actinobacillus genomosp. 1 strain CCUG22231]
ATGCAAAAACTAAATCTTATTCGACCGCTAACTAAAGCATTTACTTTAATTGAATTGATGATCGTGATTGCGATTATCGC
CATTCTTGCTACGGTTGCGATTCCGTCATACAACAGTTATACCCAAAAAGCGGCACTTTCCGAATTGTTATCGGCGTCGG
CTTCCTATAAAACGGATGTTGAAATTTGTATGTATAACACGGGAGAGGCGAAAAATTGTAGTAGCGGTCAGAACGGCGTT
AGCAAAATTACGGAGCTTAAAAGAGCTAAATATCTAAATACGATTACGGTTGAAAGCGGCACGATTACAGTGACGGGTAA
GGGGAATCTACAGGAATACGGCTATACGATGACACCGAACTATAACGGGAATACGATTTCTTGGGAAACGAAATGTAACG
GGGCGGATTTGAGTTTATTTCCGGCAAATTTCTGTGCCGCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)

Pilin (bacterial filament) (Pilin)

(43-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

70.47

100

0.709
  pilA Haemophilus influenzae 86-028NP

54.422

99.324

0.541
  pilA Haemophilus influenzae Rd KW20

53.741

99.324

0.534