Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR60_RS02265 Genome accession   NZ_CP103807
Coordinates   479853..481244 (+) Length   463 a.a.
NCBI ID   WP_279467549.1    Uniprot ID   -
Organism   Actinobacillus genomosp. 2 strain CCUG15571     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474853..486244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR60_RS02235 (NYR60_02235) - 474958..475734 (-) 777 WP_279467544.1 TSUP family transporter -
  NYR60_RS02240 (NYR60_02240) proB 475750..476841 (-) 1092 WP_279467545.1 glutamate 5-kinase -
  NYR60_RS02245 (NYR60_02245) - 476926..477414 (+) 489 WP_279467546.1 dihydrofolate reductase -
  NYR60_RS02250 (NYR60_02250) - 477539..477772 (+) 234 WP_279467547.1 YgjV family protein -
  NYR60_RS02255 (NYR60_02255) radA 477817..479195 (-) 1379 Protein_440 DNA repair protein RadA -
  NYR60_RS02260 (NYR60_02260) pilA 479387..479827 (+) 441 WP_279467548.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR60_RS02265 (NYR60_02265) pilB 479853..481244 (+) 1392 WP_279467549.1 GspE/PulE family protein Machinery gene
  NYR60_RS02270 (NYR60_02270) pilC 481231..482433 (+) 1203 WP_279467550.1 type II secretion system F family protein Machinery gene
  NYR60_RS02275 (NYR60_02275) - 482460..483104 (+) 645 WP_279467551.1 prepilin peptidase -
  NYR60_RS02280 (NYR60_02280) coaE 483126..483766 (+) 641 Protein_445 dephospho-CoA kinase -
  NYR60_RS02285 (NYR60_02285) yacG 483751..483942 (+) 192 WP_279467552.1 DNA gyrase inhibitor YacG -
  NYR60_RS02290 (NYR60_02290) - 484010..484840 (-) 831 WP_279467553.1 serine hydrolase -
  NYR60_RS02295 (NYR60_02295) - 484921..485958 (-) 1038 WP_279467554.1 glycosyltransferase family 9 protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52718.52 Da        Isoelectric Point: 6.2429

>NTDB_id=725174 NYR60_RS02265 WP_279467549.1 479853..481244(+) (pilB) [Actinobacillus genomosp. 2 strain CCUG15571]
MPYSVCDVNSQQVFEISDELWQKNFDERHILLRYLAVPVQENQQMLWLAIDDMNNLSACEIFAFIAHKQIEPVLVSSDEL
KYLLSQLMPEQRSVYEETELPYQSLESSEHFNVNDPIIRSLDNLFKYSLKHNVSDIHIEPQKEKIIIRLRIDGVLYHYKS
LSRQLSERVISRIKLLAKLDISESRLPQDGQFSFKTALSETLDFRVSSLPTHWGEKIVLRLQKNKPTHFDFLTLGFLAEQ
KAKLLKALQQPQGLILVTGPTGSGKSITLYSALNYLNDSSRHIMTAEDPVEIEIEGIIQTQVNSAIGLDFSRLLRTFLRQ
DPDVIMLGEIRDRESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIRNSLLLVIAQRLVRKLCCECAGNGC
KTCYQGYKGREGVYQILAKNGEIFDKNTAYLDFKTLHQSAEQKVQAGITSLVEIKRVLGDAEA

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=725174 NYR60_RS02265 WP_279467549.1 479853..481244(+) (pilB) [Actinobacillus genomosp. 2 strain CCUG15571]
ATGCCATATTCAGTTTGTGATGTTAACAGCCAACAGGTGTTTGAAATTTCTGATGAACTTTGGCAAAAGAATTTTGATGA
ACGTCATATTTTATTACGTTATCTCGCTGTGCCGGTTCAGGAGAATCAACAAATGTTATGGCTAGCAATAGATGATATGA
ATAATTTGAGTGCTTGTGAAATTTTTGCATTTATTGCACATAAACAAATTGAGCCGGTATTGGTTTCCTCCGATGAATTG
AAATATCTTTTAAGTCAACTAATGCCGGAACAACGATCAGTGTATGAAGAAACCGAGCTACCTTATCAATCTTTAGAATC
ATCGGAACATTTCAACGTGAATGATCCGATTATCCGGAGTTTAGATAATCTTTTTAAATATAGTTTAAAACATAATGTAT
CTGATATTCATATTGAACCGCAAAAAGAAAAGATCATCATTAGATTACGTATTGATGGTGTTCTTTATCATTATAAATCG
CTCTCAAGACAACTTTCTGAGCGAGTTATTTCCAGAATTAAGTTATTGGCGAAATTAGATATTAGTGAATCTCGCCTACC
ACAAGACGGGCAATTTAGCTTTAAAACAGCATTATCTGAAACACTCGATTTTCGGGTATCAAGTTTACCGACACATTGGG
GAGAGAAGATTGTTTTACGTTTACAAAAAAATAAACCGACGCATTTTGATTTTCTTACGTTAGGTTTTTTAGCGGAGCAG
AAAGCAAAGTTATTGAAAGCATTACAGCAACCACAAGGATTAATTTTAGTTACTGGACCTACTGGTAGCGGTAAAAGTAT
TACTTTATATAGTGCATTAAATTATTTAAATGACAGTTCCAGACATATTATGACTGCAGAAGATCCAGTAGAAATTGAAA
TCGAAGGGATCATTCAAACTCAAGTCAATTCGGCGATAGGTTTGGATTTTAGTCGTTTATTAAGAACATTTTTACGTCAA
GATCCGGATGTGATTATGTTGGGTGAAATTAGGGATAGAGAAAGTGCGGAAATTGCACTACGTGCGGCACAAACTGGTCA
TTTAGTGCTTTCCACGTTACATACGAATGATGCACCTTCCGCAATAGAACGTTTATTACAGTTAGGCATAAAAGAATATG
AGATACGTAATTCATTGCTATTAGTGATTGCTCAGCGTTTAGTCCGAAAATTATGTTGTGAATGTGCCGGCAATGGTTGT
AAGACGTGTTATCAAGGCTATAAAGGGCGTGAAGGCGTTTATCAAATCTTAGCCAAAAATGGTGAGATATTTGATAAAAA
CACAGCTTATTTAGACTTTAAAACGTTACATCAAAGTGCGGAGCAAAAAGTCCAGGCGGGTATTACCTCGTTAGTTGAAA
TAAAAAGAGTATTGGGCGATGCAGAAGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(127-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

71.116

98.704

0.702
  pilB Haemophilus influenzae Rd KW20

59.292

97.624

0.579
  pilB Haemophilus influenzae 86-028NP

58.407

97.624

0.57
  pilB Vibrio parahaemolyticus RIMD 2210633

40.58

100

0.423
  pilB Legionella pneumophila strain ERS1305867

38.114

100

0.419
  pilB Acinetobacter baylyi ADP1

39.468

100

0.417
  pilB Vibrio cholerae strain A1552

40.041

100

0.417
  pilB Acinetobacter baumannii D1279779

39.3

100

0.413
  pilB Vibrio campbellii strain DS40M4

39.501

100

0.41
  pilF Neisseria gonorrhoeae MS11

37.421

100

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.897

100

0.363