Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR60_RS02260 Genome accession   NZ_CP103807
Coordinates   479387..479827 (+) Length   146 a.a.
NCBI ID   WP_279467548.1    Uniprot ID   -
Organism   Actinobacillus genomosp. 2 strain CCUG15571     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474387..484827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR60_RS02235 (NYR60_02235) - 474958..475734 (-) 777 WP_279467544.1 TSUP family transporter -
  NYR60_RS02240 (NYR60_02240) proB 475750..476841 (-) 1092 WP_279467545.1 glutamate 5-kinase -
  NYR60_RS02245 (NYR60_02245) - 476926..477414 (+) 489 WP_279467546.1 dihydrofolate reductase -
  NYR60_RS02250 (NYR60_02250) - 477539..477772 (+) 234 WP_279467547.1 YgjV family protein -
  NYR60_RS02255 (NYR60_02255) radA 477817..479195 (-) 1379 Protein_440 DNA repair protein RadA -
  NYR60_RS02260 (NYR60_02260) pilA 479387..479827 (+) 441 WP_279467548.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR60_RS02265 (NYR60_02265) pilB 479853..481244 (+) 1392 WP_279467549.1 GspE/PulE family protein Machinery gene
  NYR60_RS02270 (NYR60_02270) pilC 481231..482433 (+) 1203 WP_279467550.1 type II secretion system F family protein Machinery gene
  NYR60_RS02275 (NYR60_02275) - 482460..483104 (+) 645 WP_279467551.1 prepilin peptidase -
  NYR60_RS02280 (NYR60_02280) coaE 483126..483766 (+) 641 Protein_445 dephospho-CoA kinase -
  NYR60_RS02285 (NYR60_02285) yacG 483751..483942 (+) 192 WP_279467552.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15884.17 Da        Isoelectric Point: 8.0875

>NTDB_id=725173 NYR60_RS02260 WP_279467548.1 479387..479827(+) (pilA) [Actinobacillus genomosp. 2 strain CCUG15571]
MQKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLSASSSYKTDVEICVYNTGDINACNSGQNGI
KKDSDLKNSKYLKSVSVTNGEIKVVGQGSLENYSYTLKPNKNGQTITWETQCSGDDTSLFPANFCS

Nucleotide


Download         Length: 441 bp        

>NTDB_id=725173 NYR60_RS02260 WP_279467548.1 479387..479827(+) (pilA) [Actinobacillus genomosp. 2 strain CCUG15571]
ATGCAAAAAATCAGATTAATTAGACCGCTTGCTAAGGCTTTTACATTAATTGAATTAATGATCGTTATCGCAATTATTGC
CATTCTTGCCACTATTGCGATTCCTTCCTATAACAGCTATACCCAAAAAGCTGCACTTTCGGAATTACTTTCCGCTTCAT
CTTCTTATAAAACCGATGTTGAAATTTGTGTGTATAACACCGGAGATATTAATGCCTGTAACAGCGGACAAAATGGGATT
AAAAAAGATAGTGATCTTAAAAATTCAAAATATCTGAAATCGGTATCGGTAACAAATGGAGAAATAAAAGTAGTTGGGCA
AGGGAGCTTAGAGAATTACAGCTATACGCTAAAACCGAATAAAAATGGACAAACTATCACGTGGGAAACTCAATGTAGTG
GCGATGATACAAGTTTATTTCCGGCAAACTTCTGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)

Pilin (bacterial filament) (Pilin)

(43-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

69.595

100

0.705
  pilA Haemophilus influenzae 86-028NP

57.931

99.315

0.575
  pilA Haemophilus influenzae Rd KW20

56.552

99.315

0.562