Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYS59_RS24400 Genome accession   NZ_CP103789
Coordinates   5236296..5237996 (+) Length   566 a.a.
NCBI ID   WP_033956582.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2718     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5231296..5242996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYS59_RS24375 nadC 5233102..5233950 (+) 849 WP_023435051.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NYS59_RS24385 - 5234131..5235060 (-) 930 WP_240462250.1 O-antigen ligase family protein -
  NYS59_RS24390 - 5235106..5235513 (-) 408 WP_316927926.1 hypothetical protein -
  NYS59_RS24395 - 5235601..5236065 (-) 465 WP_058156624.1 pilin -
  NYS59_RS24400 pilB 5236296..5237996 (+) 1701 WP_033956582.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NYS59_RS24405 pilC 5238000..5239217 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  NYS59_RS24410 pilD 5239218..5240090 (+) 873 WP_128652216.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  NYS59_RS24415 coaE 5240087..5240698 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  NYS59_RS24420 yacG 5240695..5240895 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  NYS59_RS24425 - 5240932..5241141 (-) 210 WP_003094660.1 hypothetical protein -
  NYS59_RS24430 - 5241247..5241936 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  NYS59_RS24435 - 5241933..5242403 (-) 471 WP_003094664.1 hypothetical protein -
  NYS59_RS24440 - 5242400..5242825 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62313.20 Da        Isoelectric Point: 5.5798

>NTDB_id=725080 NYS59_RS24400 WP_033956582.1 5236296..5237996(+) (pilB) [Pseudomonas aeruginosa strain 2718]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=725080 NYS59_RS24400 WP_033956582.1 5236296..5237996(+) (pilB) [Pseudomonas aeruginosa strain 2718]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCACCAGGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCTATGGACGCTCTCGGCGACCTCGGTGACATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGGCGGATCAAGATGCGTGTGTCGAAGAACAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCTGCGCTCAAACAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTACGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCATTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.79

100

0.569
  pilB Legionella pneumophila strain ERS1305867

54.497

100

0.546
  pilB Vibrio cholerae strain A1552

51.264

97.88

0.502
  pilF Neisseria gonorrhoeae MS11

50.177

99.647

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

52.434

94.346

0.495
  pilB Vibrio campbellii strain DS40M4

51.22

94.17

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.727

97.173

0.376