Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PUL49_RS25730 Genome accession   NZ_CP118641
Coordinates   5533151..5533615 (-) Length   154 a.a.
NCBI ID   WP_023129290.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain P23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5528151..5538615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUL49_RS25705 (PUL49_25690) nadC 5529454..5530302 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUL49_RS25715 (PUL49_25700) - 5530483..5531394 (-) 912 Protein_5085 O-antigen ligase family protein -
  PUL49_RS25720 (PUL49_25705) - 5531540..5532520 (-) 981 WP_024086516.1 IS5-like element ISPa26 family transposase -
  PUL49_RS25725 (PUL49_25710) - 5532577..5533062 (-) 486 WP_273658694.1 hypothetical protein -
  PUL49_RS25730 (PUL49_25715) pilA/pilAI 5533151..5533615 (-) 465 WP_023129290.1 pilin Machinery gene
  PUL49_RS25735 (PUL49_25720) pilB 5533846..5535546 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUL49_RS25740 (PUL49_25725) pilC 5535550..5536767 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  PUL49_RS25745 (PUL49_25730) pilD 5536768..5537640 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PUL49_RS25750 (PUL49_25735) coaE 5537637..5538248 (+) 612 WP_033982615.1 dephospho-CoA kinase -
  PUL49_RS25755 (PUL49_25740) yacG 5538245..5538445 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16148.47 Da        Isoelectric Point: 8.9986

>NTDB_id=723317 PUL49_RS25730 WP_023129290.1 5533151..5533615(-) (pilA/pilAI) [Pseudomonas aeruginosa strain P23]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGRSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=723317 PUL49_RS25730 WP_023129290.1 5533151..5533615(-) (pilA/pilAI) [Pseudomonas aeruginosa strain P23]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364