Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NVB75_RS22160 Genome accession   NZ_CP102929
Coordinates   4838448..4839785 (+) Length   445 a.a.
NCBI ID   WP_258348712.1    Uniprot ID   -
Organism   Pseudomonas sp. CBS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4833448..4844785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NVB75_RS22140 (NVB75_22140) - 4833478..4835943 (-) 2466 WP_375378949.1 penicillin-binding protein 1A -
  NVB75_RS22145 (NVB75_22145) pilM 4836164..4836817 (+) 654 WP_258343321.1 type IV pilus biogenesis protein PilM -
  NVB75_RS22150 (NVB75_22150) - 4836817..4837368 (+) 552 WP_258343322.1 PilN domain-containing protein -
  NVB75_RS22155 (NVB75_22155) - 4837365..4838378 (+) 1014 WP_258343323.1 pilus assembly protein PilP -
  NVB75_RS22160 (NVB75_22160) pilQ 4838448..4839785 (+) 1338 WP_258348712.1 type IV pilus secretin PilQ Machinery gene
  NVB75_RS22165 (NVB75_22165) aroK 4839790..4840308 (+) 519 WP_003209345.1 shikimate kinase AroK -
  NVB75_RS22170 (NVB75_22170) aroB 4840456..4841556 (+) 1101 WP_258343324.1 3-dehydroquinate synthase -
  NVB75_RS22175 (NVB75_22175) - 4841567..4843132 (+) 1566 WP_258343325.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 48591.62 Da        Isoelectric Point: 4.7671

>NTDB_id=719803 NVB75_RS22160 WP_258348712.1 4838448..4839785(+) (pilQ) [Pseudomonas sp. CBS]
MVMAVPNRIDLIHLPPPGNTPLSTDRVAFTGDKLSLNFQNIEVRAVLQQIADVAGLNLVASDDVQGAITLRLKDVPWDQA
LDLVLQTKGLDKRVTANVLLVAPAEELAARELLVLESRKQMTELAPLRRELLQVNYAKASDLAKLFQSAVGLEGATDERG
SVAVDDRTNNIIAYQTQERLEELRKIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGQMNRGNWSAGGIRKPLAEGA
EPPENPPSSPFVDMGTVNGSAGLGIAFITDNLLLDLELSAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSTG
ATSVSFKEASLSLEVTPQITPDGWVNMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVRDGETIVIGGVFSNTQSKVVD
KVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=719803 NVB75_RS22160 WP_258348712.1 4838448..4839785(+) (pilQ) [Pseudomonas sp. CBS]
ATGGTCATGGCTGTGCCCAATCGAATCGACCTGATTCACCTGCCGCCACCGGGCAATACGCCTCTGAGTACCGATCGCGT
GGCATTTACCGGCGACAAGCTGTCCCTCAACTTCCAGAACATCGAAGTGCGCGCGGTGTTGCAGCAGATTGCCGACGTTG
CCGGCCTCAACCTGGTGGCCAGCGACGACGTGCAGGGGGCGATCACCTTGCGCCTCAAGGACGTGCCTTGGGACCAGGCG
CTGGACCTGGTGCTGCAAACCAAGGGGTTGGATAAACGTGTGACGGCCAATGTGCTGCTGGTCGCGCCGGCCGAGGAGTT
GGCCGCCCGGGAGCTGCTGGTGCTGGAGTCGCGCAAGCAAATGACCGAGCTGGCGCCGCTGCGCCGTGAGCTGCTGCAAG
TGAATTACGCCAAGGCTTCGGACCTGGCCAAGCTCTTCCAGTCGGCCGTGGGCCTGGAAGGTGCCACCGATGAGCGCGGC
TCGGTGGCGGTGGATGATCGCACCAACAACATCATCGCCTACCAGACCCAGGAGCGGCTGGAGGAGCTGCGCAAGATTGT
GGCGCAACTGGATATTCCGGTAAGACAGGTGATGATCGAGGCGCGGATCGTTGAGGCCAATGTGGATTACGACAAAAGCC
TGGGGGTGCGCTGGGGCGGGCAGATGAATCGGGGTAACTGGAGCGCTGGCGGGATCAGGAAACCGCTGGCCGAGGGCGCC
GAGCCGCCTGAAAACCCGCCCAGCTCGCCCTTTGTTGATATGGGCACGGTCAACGGCAGTGCCGGCCTGGGGATCGCTTT
TATCACCGACAACCTGTTGCTGGACCTGGAGCTTAGCGCCATGGAAAAAACCGGTAACGGTGAAATCGTCTCACAGCCCA
AAGTCGTCACTTCCGACAAGGAAACCGCACGAATCCTCAAGGGCACCGAGATTCCCTATCAGGAATCCAGCTCCACCGGC
GCCACCTCGGTGTCGTTCAAGGAAGCCTCGCTGTCATTGGAGGTAACGCCGCAAATCACCCCGGACGGCTGGGTGAACAT
GGAGGTCAAGGTCACCAAGGACGAGCCCGACTACCTGAACAAGCTCAATGACGTGCCGCCGATCAAGAAAAACGAGGTCA
ACGCCAAGGTGCTGGTGAGGGACGGCGAGACCATCGTGATTGGCGGGGTTTTCTCCAATACTCAAAGCAAAGTGGTAGAT
AAAGTGCCATTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGGGATGTGGTTTCGGAGAAAAAATCCGAGCT
GCTGGTATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(57-103)

Bacterial type II and III secretion system protein (Secretin)

(280-436)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.364

98.876

0.706
  comQ Acinetobacter baylyi ADP1

42.38

100

0.456
  pilQ Acinetobacter baumannii strain A118

42.672

100

0.445
  pilQ Acinetobacter baumannii D1279779

42.672

100

0.445
  pilQ Vibrio campbellii strain DS40M4

46.336

95.056

0.44
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

45.933

93.933

0.431
  pilQ Vibrio cholerae strain A1552

45.933

93.933

0.431
  pilQ Legionella pneumophila strain Lp02

44.762

94.382

0.422
  pilQ Legionella pneumophila strain ERS1305867

44.762

94.382

0.422