Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NQE15_RS16420 Genome accession   NZ_CP102486
Coordinates   3283309..3284505 (-) Length   398 a.a.
NCBI ID   WP_265942760.1    Uniprot ID   -
Organism   Dechloromonas sp. A34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3278309..3289505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQE15_RS16390 - 3279291..3279839 (-) 549 WP_265942749.1 peroxiredoxin-like family protein -
  NQE15_RS16395 - 3279982..3280806 (+) 825 WP_265942750.1 AraC family transcriptional regulator -
  NQE15_RS16400 - 3280865..3281716 (-) 852 WP_265942752.1 SDR family oxidoreductase -
  NQE15_RS16405 - 3281807..3282202 (+) 396 WP_265942754.1 winged helix-turn-helix transcriptional regulator -
  NQE15_RS16410 - 3282218..3282496 (+) 279 WP_265942756.1 hypothetical protein -
  NQE15_RS16415 - 3282405..3283259 (+) 855 WP_265942758.1 alpha/beta hydrolase -
  NQE15_RS16420 pilU 3283309..3284505 (-) 1197 WP_265942760.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NQE15_RS16425 - 3284652..3284780 (+) 129 WP_265942761.1 hypothetical protein -
  NQE15_RS16430 - 3284808..3284984 (-) 177 WP_265942763.1 hypothetical protein -
  NQE15_RS16435 - 3284984..3285820 (-) 837 WP_265942765.1 dihydroorotate dehydrogenase-like protein -
  NQE15_RS16440 nifJ 3285813..3289433 (-) 3621 WP_265942767.1 pyruvate:ferredoxin (flavodoxin) oxidoreductase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 43630.05 Da        Isoelectric Point: 6.4443

>NTDB_id=717255 NQE15_RS16420 WP_265942760.1 3283309..3284505(-) (pilU) [Dechloromonas sp. A34]
MNNEDVIGDELKNYIHALLKAMVQHGGSDLFVARDFPPTMKVHGTMKALSAQKLTADISRKLALILMNHQQREDFDREME
CNFAISLPGIARFRVNVFQQQAAVSMVIRTITSEIPNFDKLGLPPILREVISAKRGLVLVVGGTGSGKSTTMAAMIDHRN
STAPGHIITVEDPVEYVHVSKQCLISHREVGVDTLSWHNALKNTLRQAPDVILIGEIRDMETMEHALAFAETGHLCMGTL
HANNANQTLDRVVNFFPDERKAQVMMDLASNLKAVVSQRLVRSIDGKRKAAIEILLNTPTVAELLSKGQSAAIKPVMDKS
RELGMQTFDQALFDLYDAGHISYDEAIRNADSTNELRLAIKLKSKKGEPSTASGLSLSMEEKEEPPAEAAAEEAASAG

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=717255 NQE15_RS16420 WP_265942760.1 3283309..3284505(-) (pilU) [Dechloromonas sp. A34]
ATGAACAACGAAGACGTGATCGGCGACGAGCTAAAGAATTACATCCATGCCTTGCTCAAGGCGATGGTTCAGCATGGCGG
CTCCGATCTGTTTGTCGCGCGCGACTTTCCGCCGACCATGAAGGTGCACGGCACGATGAAGGCGTTGTCGGCGCAGAAGC
TGACCGCCGACATTTCGCGCAAGCTGGCGCTGATCCTGATGAATCACCAGCAACGCGAAGATTTCGACCGCGAGATGGAG
TGCAATTTCGCCATTTCATTGCCCGGGATCGCCCGTTTCCGCGTCAATGTCTTCCAGCAGCAGGCGGCGGTCAGCATGGT
CATCCGCACCATCACCAGCGAAATTCCCAACTTCGACAAGCTCGGCCTGCCGCCGATCCTGCGTGAGGTAATCAGCGCCA
AGCGCGGCCTGGTGCTGGTCGTGGGCGGCACCGGCTCCGGCAAGTCGACGACCATGGCGGCGATGATCGATCACCGCAAC
TCAACGGCGCCCGGCCACATCATCACCGTCGAAGACCCGGTCGAGTACGTCCATGTCTCGAAGCAATGCCTGATATCGCA
CCGGGAAGTCGGGGTCGATACGCTGTCCTGGCACAACGCCCTGAAGAACACGCTGCGCCAGGCGCCGGACGTCATCCTGA
TCGGTGAAATCCGCGACATGGAAACCATGGAACACGCGCTGGCCTTTGCTGAAACCGGCCACCTGTGCATGGGCACGCTG
CACGCCAACAACGCCAACCAGACGCTCGACCGGGTGGTCAATTTCTTTCCCGACGAACGCAAGGCGCAGGTCATGATGGA
CCTGGCTTCCAACCTCAAGGCCGTCGTTTCCCAGCGCTTGGTCCGCTCGATAGACGGCAAGCGCAAGGCGGCCATCGAAA
TCCTGCTCAACACGCCGACCGTGGCCGAACTGCTTTCCAAGGGGCAGTCGGCCGCGATCAAGCCGGTGATGGACAAGAGC
CGCGAGCTGGGCATGCAGACCTTCGACCAAGCCCTGTTCGACCTCTACGACGCCGGCCACATCAGCTACGACGAGGCGAT
CCGCAACGCCGACTCGACCAATGAACTGCGGCTGGCCATCAAGCTGAAGAGCAAGAAAGGCGAGCCGTCGACCGCAAGCG
GCCTGTCACTGTCGATGGAAGAAAAGGAAGAGCCGCCCGCCGAAGCCGCCGCCGAAGAGGCGGCCAGCGCCGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(91-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.941

93.97

0.497
  pilU Acinetobacter baylyi ADP1

52.825

88.945

0.47
  pilU Vibrio cholerae strain A1552

48.229

92.211

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

87.186

0.387
  pilT Pseudomonas aeruginosa PAK

43.976

83.417

0.367
  pilT Pseudomonas stutzeri DSM 10701

43.452

84.422

0.367
  pilT Legionella pneumophila strain Lp02

43.902

82.412

0.362
  pilT Legionella pneumophila strain ERS1305867

43.902

82.412

0.362