Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NUT31_RS14080 Genome accession   NZ_CP102454
Coordinates   3131147..3132853 (+) Length   568 a.a.
NCBI ID   WP_268013897.1    Uniprot ID   -
Organism   Aeromonas sp. BC14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3126147..3137853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUT31_RS14055 (NUT31_14050) pdhR 3127294..3128058 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NUT31_RS14060 (NUT31_14055) ampD 3128334..3128906 (-) 573 WP_268013894.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NUT31_RS14065 (NUT31_14060) - 3129053..3129523 (+) 471 WP_024944772.1 retropepsin-like aspartic protease -
  NUT31_RS14070 (NUT31_14065) nadC 3129529..3130386 (+) 858 WP_268013895.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NUT31_RS14075 (NUT31_14070) - 3130709..3131143 (+) 435 WP_268013896.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NUT31_RS14080 (NUT31_14075) pilB 3131147..3132853 (+) 1707 WP_268013897.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NUT31_RS14085 (NUT31_14080) pilC 3132969..3134210 (+) 1242 WP_268013898.1 type II secretion system F family protein Machinery gene
  NUT31_RS14090 (NUT31_14085) pilD 3134233..3135105 (+) 873 WP_268013899.1 A24 family peptidase Machinery gene
  NUT31_RS14095 (NUT31_14090) coaE 3135124..3135738 (+) 615 WP_268013901.1 dephospho-CoA kinase -
  NUT31_RS14100 (NUT31_14095) zapD 3135776..3136498 (+) 723 WP_024945431.1 cell division protein ZapD -
  NUT31_RS14105 (NUT31_14100) yacG 3136508..3136702 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  NUT31_RS14110 (NUT31_14105) mutT 3136763..3137176 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62495.90 Da        Isoelectric Point: 5.4342

>NTDB_id=717127 NUT31_RS14080 WP_268013897.1 3131147..3132853(+) (pilB) [Aeromonas sp. BC14]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPIGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=717127 NUT31_RS14080 WP_268013897.1 3131147..3132853(+) (pilB) [Aeromonas sp. BC14]
ATGACCTCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCGCAGCGTAAACCCTTTGTGACCTTTCTCATCGAAAATGAAATCCTCGACAGCAAGGCATTGG
CTGACTTCTGCGAGCTAGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGTTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCTACCAATGTGTCGGCACTGGAGGATTTCGGGTTCAGCTTCGGGTTGCACACCGAGGCTCTGCTGGTCGAGGAGAACA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATT
TCCGAACTGGAGGTGTCGGATGAGGGGTCGCGACTCGACGAGAGCGTCAATACGGCCGACGATGACGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGAGGGGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGATAGCAACCCCACCGGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAACGGCGACTGCCTCAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAGGCGCAATATCTGCGTGCTCTCTCTAAGCCTCAG
GGCATGATCCTAGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACTGCCGAAGATCCGGTGGAAATAAATCTGCCCGGCGTCAATCAGGTGCAAGTCAACCCCAAGG
CAGGACTGACCTTCGCCAGTGCCCTGCGCTCCTTTCTGCGCCAGGATCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACTCTGATCATGGCTCAGC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCGGGCTTGCGGCTGTTCAAACCGATTGGCTGCAAAGAGTGCTCGGGTGGGTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAATATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCTGGCCTTGAGAAGGCCCGCCTTGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.277

99.296

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375