Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   G314FT_RS02475 Genome accession   NZ_CP102451
Coordinates   484118..484621 (-) Length   167 a.a.
NCBI ID   WP_257701938.1    Uniprot ID   -
Organism   Vagococcus luciliae strain G314F     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 471499..516188 484118..484621 within 0


Gene organization within MGE regions


Location: 471499..516188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G314FT_RS02415 (G314FT_04860) - 471499..472152 (-) 654 WP_257701928.1 hypothetical protein -
  G314FT_RS02420 (G314FT_04870) - 472165..473247 (-) 1083 WP_257701929.1 DUF916 and DUF3324 domain-containing protein -
  G314FT_RS02425 (G314FT_04880) - 473345..474244 (-) 900 WP_257701930.1 WxL domain-containing protein -
  G314FT_RS02430 (G314FT_04890) - 474273..475073 (-) 801 WP_257701931.1 WxL domain-containing protein -
  G314FT_RS02435 (G314FT_04900) - 475070..475420 (-) 351 WP_257701932.1 hypothetical protein -
  G314FT_RS02440 (G314FT_04910) - 475444..477915 (-) 2472 WP_257701933.1 hypothetical protein -
  G314FT_RS02445 (G314FT_04920) - 478237..479178 (+) 942 WP_257701934.1 IS30 family transposase -
  G314FT_RS02450 (G314FT_04930) - 479503..480375 (-) 873 WP_257701935.1 DegV family protein -
  G314FT_RS02455 (G314FT_04940) - 480470..481762 (-) 1293 WP_257701936.1 adenylosuccinate synthase -
  G314FT_RS02460 (G314FT_04950) dnaB 481885..483252 (-) 1368 WP_257701937.1 replicative DNA helicase -
  G314FT_RS02465 (G314FT_04960) rplI 483276..483728 (-) 453 WP_125958308.1 50S ribosomal protein L9 -
  G314FT_RS02470 (G314FT_04970) rpsR 483860..484099 (-) 240 WP_071455937.1 30S ribosomal protein S18 -
  G314FT_RS02475 (G314FT_04980) ssb 484118..484621 (-) 504 WP_257701938.1 single-stranded DNA-binding protein Machinery gene
  G314FT_RS02480 (G314FT_04990) rpsF 484665..484964 (-) 300 WP_125958310.1 30S ribosomal protein S6 -
  G314FT_RS02485 (G314FT_05000) gyrA 485278..487794 (-) 2517 WP_257701939.1 DNA gyrase subunit A -
  G314FT_RS02490 (G314FT_05010) gyrB 487812..489758 (-) 1947 WP_257702484.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  G314FT_RS02495 (G314FT_05020) recF 489748..490881 (-) 1134 WP_257701940.1 DNA replication/repair protein RecF -
  G314FT_RS02500 (G314FT_05030) yaaA 490884..491117 (-) 234 WP_117973751.1 S4 domain-containing protein YaaA -
  G314FT_RS02505 (G314FT_05040) - 491354..491827 (-) 474 WP_257701941.1 glutathione peroxidase -
  G314FT_RS02510 (G314FT_05050) dnaN 491960..493090 (-) 1131 WP_257701942.1 DNA polymerase III subunit beta -
  G314FT_RS02515 (G314FT_05060) dnaA 493246..494589 (-) 1344 WP_257701943.1 chromosomal replication initiator protein DnaA -
  G314FT_RS02520 (G314FT_05070) rpmH 495064..495198 (+) 135 WP_071457793.1 50S ribosomal protein L34 -
  G314FT_RS02525 (G314FT_05080) rnpA 495284..495622 (+) 339 WP_257701944.1 ribonuclease P protein component -
  G314FT_RS02530 (G314FT_05090) - 495673..496455 (+) 783 WP_257702486.1 YidC/Oxa1 family membrane protein insertase -
  G314FT_RS02535 (G314FT_05100) jag 496474..497235 (+) 762 WP_257701945.1 RNA-binding cell elongation regulator Jag/EloR -
  G314FT_RS02540 (G314FT_05110) mnmE 497444..498838 (+) 1395 WP_257701946.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  G314FT_RS02545 (G314FT_05120) mnmG 498853..500751 (+) 1899 WP_257701947.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  G314FT_RS02550 (G314FT_05130) - 500825..502003 (-) 1179 WP_257701948.1 site-specific integrase -
  G314FT_RS02555 (G314FT_05140) - 502060..502608 (-) 549 WP_257701949.1 helix-turn-helix transcriptional regulator -
  G314FT_RS02560 (G314FT_05150) - 502758..502955 (+) 198 WP_257701950.1 helix-turn-helix transcriptional regulator -
  G314FT_RS02565 (G314FT_05160) - 502993..503271 (+) 279 WP_257701951.1 helix-turn-helix domain-containing protein -
  G314FT_RS02570 (G314FT_05170) - 503320..503577 (+) 258 WP_257701952.1 hypothetical protein -
  G314FT_RS02575 (G314FT_05180) - 503588..504436 (+) 849 WP_257701953.1 bifunctional DNA primase/polymerase -
  G314FT_RS02580 (G314FT_05190) - 504433..505836 (+) 1404 WP_257701954.1 phage/plasmid primase, P4 family -
  G314FT_RS02585 (G314FT_05200) - 506195..508087 (+) 1893 WP_257701955.1 head maturation protease, ClpP-related -
  G314FT_RS02590 (G314FT_05210) - 508285..508926 (+) 642 WP_257701956.1 hypothetical protein -
  G314FT_RS02595 (G314FT_05220) - 509242..509850 (+) 609 WP_257701957.1 hypothetical protein -
  G314FT_RS02600 (G314FT_05230) rsmG 510027..510746 (+) 720 WP_257701958.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  G314FT_RS02605 (G314FT_05240) - 510798..511568 (+) 771 WP_257701959.1 AAA family ATPase -
  G314FT_RS02610 (G314FT_05250) - 511552..512436 (+) 885 WP_257701960.1 ParB/RepB/Spo0J family partition protein -
  G314FT_RS02615 (G314FT_05260) - 512531..512716 (+) 186 WP_257701961.1 DUF951 domain-containing protein -
  G314FT_RS02620 (G314FT_05270) ychF 512751..513851 (+) 1101 WP_257701962.1 redox-regulated ATPase YchF -
  G314FT_RS02625 (G314FT_05280) - 513873..514574 (+) 702 WP_257702488.1 DUF1129 domain-containing protein -
  G314FT_RS02630 (G314FT_05290) guaB 514707..516188 (+) 1482 WP_079346431.1 IMP dehydrogenase -

Sequence

Protein


Download         Length: 167 a.a.        Molecular weight: 18691.32 Da        Isoelectric Point: 4.6968

>NTDB_id=717058 G314FT_RS02475 WP_257701938.1 484118..484621(-) (ssb) [Vagococcus luciliae strain G314F]
MINNVVLVGRLTRDPDLRYTSNGSAVATFNLAVNRNFTNQSGEREADFVNCVIWRKPAETLANYAKKGTLLGVVGRIQTR
NYENQQGQRVYVTEVVCENFQLLESKNASSQRQQQTSGFNNFSQDNQNTQSSFGQSSSNDMPNFDRDNSNPFGNSSSIDI
SDDDLPF

Nucleotide


Download         Length: 504 bp        

>NTDB_id=717058 G314FT_RS02475 WP_257701938.1 484118..484621(-) (ssb) [Vagococcus luciliae strain G314F]
ATGATTAATAATGTTGTATTGGTAGGTAGACTTACTCGAGACCCAGATTTGAGATATACGTCAAATGGTTCTGCCGTAGC
CACTTTTAACTTAGCTGTTAATCGTAATTTTACTAATCAAAGTGGAGAACGAGAAGCAGATTTTGTTAACTGTGTTATTT
GGAGAAAACCAGCTGAAACTCTAGCTAATTATGCTAAAAAAGGGACTCTATTAGGAGTAGTTGGACGTATTCAAACTAGA
AACTATGAGAATCAACAGGGACAAAGAGTGTATGTAACTGAAGTGGTTTGTGAGAACTTCCAATTATTAGAATCTAAGAA
TGCATCAAGTCAAAGACAACAGCAAACTAGTGGATTTAATAATTTTTCTCAAGATAACCAAAATACACAATCATCATTTG
GCCAATCTTCAAGTAATGACATGCCGAACTTCGATCGTGATAATAGTAATCCTTTTGGAAACTCATCATCGATAGATATT
TCGGATGACGATTTACCATTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

61.765

100

0.629
  ssbA Bacillus subtilis subsp. subtilis str. 168

57.558

100

0.593
  ssbB Bacillus subtilis subsp. subtilis str. 168

62.264

63.473

0.395
  ssbB/cilA Streptococcus pneumoniae TIGR4

50.4

74.85

0.377
  ssbB/cilA Streptococcus pneumoniae Rx1

49.6

74.85

0.371
  ssbB/cilA Streptococcus pneumoniae D39

49.6

74.85

0.371
  ssbB/cilA Streptococcus pneumoniae R6

49.6

74.85

0.371
  ssbB/cilA Streptococcus mitis NCTC 12261

49.6

74.85

0.371
  ssbB/cilA Streptococcus mitis SK321

49.6

74.85

0.371