Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NR756_RS17810 Genome accession   NZ_CP102389
Coordinates   3856055..3857767 (+) Length   570 a.a.
NCBI ID   WP_233917136.1    Uniprot ID   -
Organism   Alloalcanivorax xenomutans strain AX     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3851055..3862767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NR756_RS17790 (NR756_17765) - 3851509..3851985 (+) 477 WP_284456056.1 DUF1850 domain-containing protein -
  NR756_RS17795 (NR756_17770) - 3851982..3854192 (+) 2211 WP_416391152.1 TRAP transporter permease -
  NR756_RS17800 (NR756_17775) - 3854574..3855041 (+) 468 WP_416391153.1 hypothetical protein -
  NR756_RS17805 (NR756_17780) - 3855223..3855693 (-) 471 WP_233917135.1 pilin -
  NR756_RS17810 (NR756_17785) pilB 3856055..3857767 (+) 1713 WP_233917136.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NR756_RS17815 (NR756_17790) pilC 3857838..3859061 (+) 1224 WP_233917137.1 type II secretion system F family protein Machinery gene
  NR756_RS17820 (NR756_17795) pilD 3859089..3859952 (+) 864 WP_022995250.1 prepilin peptidase Machinery gene
  NR756_RS17825 (NR756_17800) coaE 3860166..3860771 (+) 606 WP_022995249.1 dephospho-CoA kinase -
  NR756_RS17830 (NR756_17805) - 3860761..3860955 (+) 195 WP_022995248.1 DNA gyrase inhibitor YacG -
  NR756_RS17835 (NR756_17810) - 3860956..3861906 (-) 951 WP_416391154.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62339.18 Da        Isoelectric Point: 5.6276

>NTDB_id=716785 NR756_RS17810 WP_233917136.1 3856055..3857767(+) (pilB) [Alloalcanivorax xenomutans strain AX]
MSSPQSSLSGLARRLVNEGHLSTEQVMEAAGAARREQMPLVRRLVGSGLLTAATVARASAEEFGDPVIDLDAFSPDVLVK
DLVDTKLIARHTVLPLYKRGSRLFVAVSDPTNLPALEEIRFNTGLNVETVIVEDDKLTRWIDRLESEAEGKAFADLDEGL
DNLDVANGEPAADAKDEANGADDAPIVRFVNKLLLDAIKGGASDLHFEPYEKTYRVRFRQDGVLQTMSKPPINSASKIAA
RLKVMARLDISERRVPQDGRIKLKISKNRAIDFRVNTCPTLFGEKIVLRILDPDSAKMGIDALGYEAEQKDMYLRALNQP
QGMILVTGPTGSGKTVSLYTGVNILNTPERNISTAEDPVEINLEGINQVNVNPRQGLDFSSALRAFLRQDPDVILVGEIR
DLETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVSLIIAQRLARRLCSHCKEAVEVPKPSLLAMGF
TEQDIESGFTVFGPKGCQKCNNGYKGRVGIYEVVRVTDGIARIIMQDGNSIEIADQCRKEGFNDLRRSGLLKVMQGVTSL
EEMDRVTSGH

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=716785 NR756_RS17810 WP_233917136.1 3856055..3857767(+) (pilB) [Alloalcanivorax xenomutans strain AX]
ATGAGCAGTCCTCAATCATCCCTGAGCGGCCTCGCCCGCCGCCTGGTCAACGAAGGCCATCTGTCCACCGAGCAGGTGAT
GGAAGCGGCCGGCGCCGCCCGCCGGGAGCAGATGCCGCTGGTCCGGCGTCTGGTGGGCTCGGGTCTGCTGACCGCGGCCA
CCGTGGCACGGGCTTCCGCCGAGGAGTTCGGGGATCCGGTGATAGATCTGGATGCGTTTTCCCCGGATGTCCTGGTCAAG
GACCTGGTGGATACCAAGCTGATCGCCCGCCACACCGTCCTGCCCCTGTACAAAAGGGGCAGCCGTTTGTTCGTCGCCGT
CTCCGACCCCACCAATTTGCCGGCGCTGGAGGAAATCCGCTTCAATACCGGCCTCAACGTGGAAACAGTGATTGTCGAGG
ACGACAAGCTGACCCGTTGGATCGACCGGCTGGAGTCGGAAGCGGAGGGCAAGGCGTTCGCCGATCTCGACGAGGGGCTG
GACAATCTGGACGTGGCCAACGGTGAACCGGCCGCGGACGCCAAGGACGAGGCCAACGGCGCCGACGATGCCCCCATCGT
TCGCTTCGTCAACAAGCTGCTGCTGGACGCCATCAAGGGCGGCGCCTCCGACCTGCACTTCGAACCCTACGAGAAGACCT
ACCGGGTACGGTTCCGTCAGGACGGTGTGTTGCAGACCATGTCCAAACCGCCGATCAACAGCGCCAGCAAGATCGCCGCC
CGGCTCAAGGTGATGGCGCGCCTGGATATTTCCGAGCGGCGGGTTCCCCAGGACGGCCGCATCAAGCTGAAGATTTCCAA
GAATCGCGCCATCGATTTCCGGGTCAACACCTGCCCCACCCTGTTTGGCGAGAAGATCGTGCTGCGGATTCTGGATCCGG
ACAGCGCCAAGATGGGTATTGATGCTCTGGGCTATGAAGCCGAGCAAAAAGACATGTACCTGAGAGCCCTGAACCAGCCC
CAGGGCATGATCCTGGTCACCGGTCCCACCGGTTCCGGTAAAACCGTGTCTCTCTATACCGGCGTGAATATTCTCAACAC
GCCGGAGCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCAACCTGGAAGGCATCAATCAGGTCAACGTGAATCCCA
GGCAGGGGCTGGATTTCTCGTCCGCGCTGCGGGCGTTCCTGCGTCAGGATCCCGACGTCATTCTGGTGGGGGAGATCCGC
GATCTGGAGACCGCGGAAATCGCCGTGAAAGCCGCCCAAACCGGCCACCTGGTGTTATCAACCCTGCACACCAACAGCGC
TCCGGAAACCCTGACCCGTCTGCGTAACATGGGGGTGCCGTCCTTCAACATCGCCACGTCGGTGAGTCTGATCATCGCCC
AGCGCCTGGCCCGCCGCCTTTGCAGCCACTGCAAGGAAGCGGTGGAGGTGCCCAAACCCTCGCTGCTTGCGATGGGTTTC
ACGGAACAGGACATCGAGAGTGGCTTTACTGTGTTCGGCCCGAAAGGCTGCCAAAAATGCAATAACGGCTACAAGGGCCG
GGTGGGGATCTATGAGGTAGTCCGGGTCACCGACGGCATTGCCCGCATCATCATGCAGGATGGCAATTCCATCGAGATCG
CCGACCAGTGCCGCAAGGAAGGCTTCAACGATCTGCGCCGCTCCGGGCTGCTCAAGGTGATGCAGGGGGTCACCAGTCTG
GAGGAAATGGACCGGGTCACCAGCGGCCACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.39

99.825

0.623
  pilB Acinetobacter baylyi ADP1

60.924

98.772

0.602
  pilB Legionella pneumophila strain ERS1305867

52.439

100

0.528
  pilF Neisseria gonorrhoeae MS11

49.291

98.947

0.488
  pilB Vibrio cholerae strain A1552

54.403

89.649

0.488
  pilB Vibrio campbellii strain DS40M4

50.947

92.632

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

50.659

93.158

0.472
  pilB/pilB1 Synechocystis sp. PCC 6803

41.473

90.526

0.375
  pilF Thermus thermophilus HB27

38.378

97.368

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.228

92.456

0.372