Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NRG23_RS23940 Genome accession   NZ_CP102374
Coordinates   5283520..5284737 (-) Length   405 a.a.
NCBI ID   WP_257372457.1    Uniprot ID   -
Organism   Pseudomonas sp. T8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5278520..5289737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRG23_RS23910 (NRG23_23910) - 5280386..5280853 (+) 468 WP_016703672.1 hypothetical protein -
  NRG23_RS23915 (NRG23_23915) - 5280850..5281539 (+) 690 WP_110177023.1 energy-coupling factor ABC transporter permease -
  NRG23_RS23920 (NRG23_23920) - 5281605..5281820 (+) 216 WP_009050911.1 hypothetical protein -
  NRG23_RS23925 (NRG23_23925) yacG 5281825..5282028 (-) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  NRG23_RS23930 (NRG23_23930) coaE 5282025..5282648 (-) 624 WP_009045669.1 dephospho-CoA kinase -
  NRG23_RS23935 (NRG23_23935) pilD 5282645..5283517 (-) 873 WP_009050909.1 A24 family peptidase Machinery gene
  NRG23_RS23940 (NRG23_23940) pilC 5283520..5284737 (-) 1218 WP_257372457.1 type II secretion system F family protein Machinery gene
  NRG23_RS23945 (NRG23_23945) - 5285102..5285518 (+) 417 WP_009050907.1 pilin -
  NRG23_RS23950 (NRG23_23950) - 5285806..5286162 (+) 357 WP_009050906.1 BON domain-containing protein -
  NRG23_RS23955 (NRG23_23955) - 5286365..5289283 (-) 2919 WP_257372460.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44444.23 Da        Isoelectric Point: 10.4090

>NTDB_id=716618 NRG23_RS23940 WP_257372457.1 5283520..5284737(-) (pilC) [Pseudomonas sp. T8]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVRKKSSSLFQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLAELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HAHRRSPRFRHWLDAALLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=716618 NRG23_RS23940 WP_257372457.1 5283520..5284737(-) (pilC) [Pseudomonas sp. T8]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCAGCCAGGGTCAGGAAGAAATCCA
GCTCGCTGTTCCAGCGCACGCCCCCGATCAAGGCTCGCGACATCAGCCTGTTCACCCGGCAGTTGGCGACCCTGATCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGCTGGCCGA
ACTGAAACAGGACATCGCCGCCGGCAGCAGTTTCACCAGTGCGCTGCGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCTCTGTTGCTCAGCGTGTTCGGCCTGCGT
CATGCCCACCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGCGGCCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCACTTTTCCATGCGCGCGTCCGGTATCTTTCCCGGCATGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCACTACGAGGAAGAAGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAGCCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.926

100

0.659
  pilC Acinetobacter baylyi ADP1

55.882

100

0.563
  pilC Acinetobacter baumannii D1279779

55.147

100

0.556
  pilC Legionella pneumophila strain ERS1305867

52.778

97.778

0.516
  pilG Neisseria gonorrhoeae MS11

42.928

99.506

0.427
  pilG Neisseria meningitidis 44/76-A

42.184

99.506

0.42
  pilC Vibrio cholerae strain A1552

42.602

96.79

0.412
  pilC Vibrio campbellii strain DS40M4

39.552

99.259

0.393