Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRZ29_RS02290 Genome accession   NZ_CP102370
Coordinates   498146..499852 (-) Length   568 a.a.
NCBI ID   WP_146061427.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BC19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 493146..504852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ29_RS02260 (NRZ29_02260) mutT 493823..494236 (+) 414 WP_268029972.1 8-oxo-dGTP diphosphatase MutT -
  NRZ29_RS02265 (NRZ29_02265) yacG 494297..494491 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  NRZ29_RS02270 (NRZ29_02270) zapD 494501..495223 (-) 723 WP_024945431.1 cell division protein ZapD -
  NRZ29_RS02275 (NRZ29_02275) coaE 495261..495875 (-) 615 WP_268029973.1 dephospho-CoA kinase -
  NRZ29_RS02280 (NRZ29_02280) pilD 495894..496766 (-) 873 WP_268029974.1 prepilin peptidase Machinery gene
  NRZ29_RS02285 (NRZ29_02285) pilC 496789..498030 (-) 1242 WP_124251316.1 type II secretion system F family protein Machinery gene
  NRZ29_RS02290 (NRZ29_02290) pilB 498146..499852 (-) 1707 WP_146061427.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NRZ29_RS02295 (NRZ29_02295) - 499857..500297 (-) 441 WP_268029975.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NRZ29_RS02300 (NRZ29_02300) nadC 500620..501477 (-) 858 WP_268013895.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NRZ29_RS02305 (NRZ29_02305) - 501483..501953 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  NRZ29_RS02310 (NRZ29_02310) ampD 502100..502672 (+) 573 WP_268029976.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NRZ29_RS02315 (NRZ29_02315) pdhR 502948..503712 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62481.87 Da        Isoelectric Point: 5.4342

>NTDB_id=716575 NRZ29_RS02290 WP_146061427.1 498146..499852(-) (pilB) [Aeromonas hydrophila strain BC19]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=716575 NRZ29_RS02290 WP_146061427.1 498146..499852(-) (pilB) [Aeromonas hydrophila strain BC19]
ATGACCTCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCCCAGCGCAAACCTTTTGTGACCTTTCTCATCGAAAACGAAATCCTCGATAGCAAGGCTCTAG
CTGACTTCTGCGAGCTAGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGTTGATTGAAAAACATCACGTCCTGCCCATCTACACTCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCTACCAATGTGTCGGCACTGGAGGACTTTGGCTTCAGCTTCGGGTTGCACACCGAGGCTCTGCTGGTCGAGGAGAACA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGTGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATT
TCCGAACTGGAGGTGTCGGATGAGGGGTCGCGACTCGACGAGAGCGTCAATACAGCTGACGATGACGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGATAGCAACCCCACCGGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAACGGCGACTGCCTCAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAGGCGCAATATCTGCGTGCTCTCTCGAAGCCTCAG
GGCATGATCCTAGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACTGCCGAAGATCCGGTGGAAATAAATCTGCCCGGCGTCAATCAGGTGCAAGTCAACCCCAAGG
CAGGACTGACCTTCGCCAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGACGTAGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTCCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGGTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAAGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGTACCTTGCGCACCTCCGGCCTCGAGAAGGCACGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375