Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRZ32_RS16335 Genome accession   NZ_CP102368
Coordinates   3594910..3596616 (+) Length   568 a.a.
NCBI ID   WP_201892603.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3589910..3601616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ32_RS16310 (NRZ32_16310) pdhR 3591087..3591851 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NRZ32_RS16315 (NRZ32_16315) ampD 3592127..3592699 (-) 573 WP_268037595.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NRZ32_RS16320 (NRZ32_16320) - 3592823..3593293 (+) 471 WP_268037596.1 TIGR02281 family clan AA aspartic protease -
  NRZ32_RS16325 (NRZ32_16325) nadC 3593298..3594155 (+) 858 WP_043171615.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NRZ32_RS16330 (NRZ32_16330) - 3594477..3594905 (+) 429 WP_268037597.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NRZ32_RS16335 (NRZ32_16335) pilB 3594910..3596616 (+) 1707 WP_201892603.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NRZ32_RS16340 (NRZ32_16340) pilC 3596732..3597973 (+) 1242 WP_268037599.1 type II secretion system F family protein Machinery gene
  NRZ32_RS16345 (NRZ32_16345) pilD 3597996..3598868 (+) 873 WP_268037600.1 prepilin peptidase Machinery gene
  NRZ32_RS16350 (NRZ32_16350) coaE 3598888..3599502 (+) 615 WP_268037601.1 dephospho-CoA kinase -
  NRZ32_RS16355 (NRZ32_16355) zapD 3599540..3600262 (+) 723 WP_268037602.1 cell division protein ZapD -
  NRZ32_RS16360 (NRZ32_16360) yacG 3600272..3600466 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  NRZ32_RS16365 (NRZ32_16365) mutT 3600527..3600940 (-) 414 WP_010635956.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62528.91 Da        Isoelectric Point: 5.4321

>NTDB_id=716561 NRZ32_RS16335 WP_201892603.1 3594910..3596616(+) (pilB) [Aeromonas dhakensis strain BC18]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMENDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=716561 NRZ32_RS16335 WP_201892603.1 3594910..3596616(+) (pilB) [Aeromonas dhakensis strain BC18]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCACAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGACATCCTCGACAGCAAGGCGCTGG
CCGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTACTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAGGCACTTCTGGTCGAGGAGAACA
AGCTGACCGCCGCCATCAGCAAGCTGATGGAAAACGATCAGGACGCCCTCGGCATGGAGGACATCGACGAGTCCGAGATC
TCCGAGCTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTACGAGACCAAATACC
GTATCCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCAACGCCGCCGGTCAATCTGGCCAATCGCTTCTCTGCCCGC
CTCAAGGTGATGGCGCGCCTGGACATTGCCGAACGGCGGCTGCCTCAGGATGGCCGCATCAAATTGAAGATGTCGCGCAA
CAAGTCGATGGACATGCGAGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGCTTCGATGACCGTCAGAAGGCGCAATATCTGCGTGCACTCTCGAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACTGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATCCTCAACACTAC
AGAAGTAAATATTTCAACCGCCGAAGACCCGGTGGAGATCAACCTGCCGGGGGTCAACCAGGTGCAGGTCAACCCCAAGG
CCGGGCTGACCTTCGCTAGTGCCCTGCGCTCCTTCCTGCGTCAGGATCCCGACGTGGTGATGGTCGGGGAAATACGGGAC
TTGGAAACCGCTGAGATCGCCATCAAGGCGGCACAAACCGGCCACCTGGTGCTCTCCACATTGCACACCAACTCGGCGGC
CGAGACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCATGGCTCAAC
GGCTGGCCCGCAAGCTCTGTGACCACTGCAAGGCCCCCGAAGTGGTACCGGAGGCTGAACTGCTGGAATTGGGGTTTACC
CAACAACAACTGGCCGCAGGCTTGCGGCTGTTCAAACCGGTTGGTTGCAAGGAGTGCTCAGGCGGCTACAAGGGACGGGT
CGGCATCTACGAAATCCTGTTGATGTCCGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGCAGATTGCGG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGGACCTCCGGTCTTGAGAAGGCTCGACTGGGGGTGACCAGCCTGGCC
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Acinetobacter baumannii D1279779

54.833

100

0.549
  pilB Legionella pneumophila strain ERS1305867

54.417

99.648

0.542
  pilB Vibrio cholerae strain A1552

53.805

99.472

0.535
  pilB Vibrio campbellii strain DS40M4

51.59

99.648

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.944

0.504
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.53

92.958

0.377