Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRZ32_RS10875 Genome accession   NZ_CP102368
Coordinates   2425377..2426516 (+) Length   379 a.a.
NCBI ID   WP_026141098.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2420377..2431516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ32_RS10845 (NRZ32_10845) - 2420559..2420939 (-) 381 WP_005302618.1 diacylglycerol kinase -
  NRZ32_RS10850 (NRZ32_10850) - 2421096..2422157 (+) 1062 WP_005302620.1 3-deoxy-7-phosphoheptulonate synthase -
  NRZ32_RS10855 (NRZ32_10855) - 2422237..2423022 (-) 786 WP_268037128.1 ATP-binding cassette domain-containing protein -
  NRZ32_RS10860 (NRZ32_10860) btuC 2423009..2424016 (-) 1008 WP_017785953.1 vitamin B12 ABC transporter permease BtuC -
  NRZ32_RS10865 (NRZ32_10865) - 2424116..2424847 (-) 732 WP_024942579.1 substrate-binding periplasmic protein -
  NRZ32_RS10870 (NRZ32_10870) - 2424961..2425317 (+) 357 WP_005302630.1 DMT family protein -
  NRZ32_RS10875 (NRZ32_10875) pilU 2425377..2426516 (+) 1140 WP_026141098.1 type IVa pilus ATPase TapW Machinery gene
  NRZ32_RS10880 (NRZ32_10880) glgC 2426799..2428070 (+) 1272 WP_005302634.1 glucose-1-phosphate adenylyltransferase -
  NRZ32_RS10885 (NRZ32_10885) - 2428206..2428685 (+) 480 WP_268037129.1 WbuC family cupin fold metalloprotein -
  NRZ32_RS10890 (NRZ32_10890) - 2428807..2429049 (-) 243 WP_005302637.1 FeoC-like transcriptional regulator -
  NRZ32_RS10895 (NRZ32_10895) feoB 2429046..2431319 (-) 2274 WP_268037130.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42185.21 Da        Isoelectric Point: 6.2566

>NTDB_id=716553 NRZ32_RS10875 WP_026141098.1 2425377..2426516(+) (pilU) [Aeromonas dhakensis strain BC18]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=716553 NRZ32_RS10875 WP_026141098.1 2425377..2426516(+) (pilU) [Aeromonas dhakensis strain BC18]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAAAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATTTGAAACTGCCCACCGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGCT
CGGGCAAATCCACCTCGCTCGCGGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATCGGCGAAATTCGCGATCGCGAGACTATGGAGCACG
CGCTGGCCTTCTCGGAAACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACTACTGATGGCGGCCGCCGGGCGGCGGTAGAGATCATGCTGGGCACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGAGGAGGAGGAGGCAGTGAAGAACGCCGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAGCCAACCAAGGACG
AGAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

89.446

0.369
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361