Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRZ30_RS14985 Genome accession   NZ_CP102367
Coordinates   3241501..3243207 (-) Length   568 a.a.
NCBI ID   WP_033115377.1    Uniprot ID   -
Organism   Aeromonas jandaei strain BC17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3236501..3248207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ30_RS14955 (NRZ30_14955) mutT 3237148..3237561 (+) 414 WP_268016470.1 8-oxo-dGTP diphosphatase MutT -
  NRZ30_RS14960 (NRZ30_14960) yacG 3237622..3237816 (-) 195 WP_411913948.1 DNA gyrase inhibitor YacG -
  NRZ30_RS14965 (NRZ30_14965) zapD 3237826..3238548 (-) 723 WP_041210678.1 cell division protein ZapD -
  NRZ30_RS14970 (NRZ30_14970) coaE 3238593..3239201 (-) 609 WP_268016472.1 dephospho-CoA kinase -
  NRZ30_RS14975 (NRZ30_14975) pilD 3239219..3240091 (-) 873 WP_268016473.1 prepilin peptidase Machinery gene
  NRZ30_RS14980 (NRZ30_14980) pilC 3240114..3241355 (-) 1242 WP_215805988.1 type II secretion system F family protein Machinery gene
  NRZ30_RS14985 (NRZ30_14985) pilB 3241501..3243207 (-) 1707 WP_033115377.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NRZ30_RS14990 (NRZ30_14990) tapA 3243215..3243649 (-) 435 WP_268016474.1 type IVa pilus major pilin TapA -
  NRZ30_RS14995 (NRZ30_14995) nadC 3243972..3244829 (-) 858 WP_268016475.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NRZ30_RS15000 (NRZ30_15000) - 3244833..3245303 (-) 471 WP_033115375.1 TIGR02281 family clan AA aspartic protease -
  NRZ30_RS15005 (NRZ30_15005) ampD 3245426..3245995 (+) 570 WP_268016476.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NRZ30_RS15010 (NRZ30_15010) pdhR 3246274..3247038 (+) 765 WP_033115373.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62564.96 Da        Isoelectric Point: 5.3215

>NTDB_id=716535 NRZ30_RS14985 WP_033115377.1 3241501..3243207(-) (pilB) [Aeromonas jandaei strain BC17]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILESKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDESSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYEQKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRMSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=716535 NRZ30_RS14985 WP_033115377.1 3241501..3243207(-) (pilB) [Aeromonas jandaei strain BC17]
ATGACTTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATAAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAAGCCAAGGCTCAGCGTAAACCCTTTGTGACCTTCCTGATCGAGAACGAGATCCTTGAGAGCAAGGCTCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTTGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAGCATCATGTGCTGCCTATCTACACGCAAGGGCACACCCTCTATATCGCCATGTCCGA
CCCGACCAATGTGTCGGCACTGGAAGATTTCGGCTTCAGCTTTGGCCTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AGCTCACAGCGGCAATAGGAAAATTGCTGGAGAGTGAGAACGATGCTCTCGGAATGGAGGATATCGATGAATCTGAAATA
TCCGACCTCGAAGTATCCGATGAGAGCAGCAGGCTCGATGAGAGCGTCAATACCGCTGATGATGATGCTCCCATTGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGTGGCGCCTCCGACCTGCACTTTGAACCCTACGAGCAGAAATACC
GCATTCGATTTCGTATCGACGGCATCCTGCATGAAGTGGCAACCCCGCCAGTCAATCTGGCCAACCGTTTCTCCGCACGC
CTCAAGGTAATGGCGCGCCTCGATATCGCCGAGCGTCGCCTGCCGCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGTAA
CAAGTCGATGGATATGCGGGTCAATACTCTGCCCACCATGTGGGGGGAGAAAATTGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAATATCTGCGTGCACTGTCGAAACCTCAA
GGCATGATCTTGGTCACCGGCCCGACCGGTTCGGGTAAAACGGTCTCGCTCTATACCGGTCTCAATATTCTCAACACTAC
CGAGGTGAATATCTCCACCGCTGAAGATCCGGTGGAGATCAACTTGCCCGGCGTCAATCAGGTGCAGGTCAATCCCAAGG
CTGGGCTGACCTTTGCCAGTGCCCTGCGCTCCTTTCTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAGATCCGCGAT
CTGGAGACGGCAGAAATAGCTATCAAGGCCGCCCAGACTGGTCACCTGGTGCTCTCTACCTTGCACACCAACTCCGCCGC
CGAAACCCTCACCCGGATGATGAACATGGGGGTGCCAGCCTTTAATATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCACGCAAACTGTGTGACCACTGCAAGGCTCCCGAAGTAGTGCCGGAAGCAGAATTACTGGAGCTTGGTTTTACT
CAGCAGCAACTGGCGGCGGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGCTCTGGTGGATACAAGGGGCGGGT
CGGTATCTACGAGATCATGCTGATGTCGGAAAACATCGCAAAACTGATCATGCAGGGGGCCAACTCGCTACAAATAGCGG
CTATAGCCCAGAAAGAGGGGATGCGTACCTTGCGTATGTCCGGATTGGAAAAGGCTCGCCTTGGCGTCACCAGTCTGGCA
GAGGTCAACCGGGTGACAACGAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

50.871

100

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.241

99.296

0.509
  pilF Neisseria gonorrhoeae MS11

46.986

99.296

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

38.596

100

0.387
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361