Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRZ31_RS12225 Genome accession   NZ_CP102365
Coordinates   2651220..2652926 (+) Length   568 a.a.
NCBI ID   WP_005305646.1    Uniprot ID   K1JG90
Organism   Aeromonas dhakensis strain BC15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2646220..2657926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ31_RS12200 (NRZ31_12200) pdhR 2647371..2648135 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NRZ31_RS12205 (NRZ31_12205) ampD 2648411..2648983 (-) 573 WP_010635964.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NRZ31_RS12210 (NRZ31_12210) - 2649107..2649577 (+) 471 WP_010635963.1 TIGR02281 family clan AA aspartic protease -
  NRZ31_RS12215 (NRZ31_12215) nadC 2649582..2650439 (+) 858 WP_010635962.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NRZ31_RS12220 (NRZ31_12220) tapA 2650761..2651213 (+) 453 WP_005305643.1 type IVa pilus major pilin TapA -
  NRZ31_RS12225 (NRZ31_12225) pilB 2651220..2652926 (+) 1707 WP_005305646.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NRZ31_RS12230 (NRZ31_12230) pilC 2653042..2654283 (+) 1242 WP_010635960.1 type II secretion system F family protein Machinery gene
  NRZ31_RS12235 (NRZ31_12235) pilD 2654306..2655178 (+) 873 WP_017766419.1 prepilin peptidase Machinery gene
  NRZ31_RS12240 (NRZ31_12240) coaE 2655198..2655812 (+) 615 WP_017766418.1 dephospho-CoA kinase -
  NRZ31_RS12245 (NRZ31_12245) zapD 2655850..2656572 (+) 723 WP_026141277.1 cell division protein ZapD -
  NRZ31_RS12250 (NRZ31_12250) yacG 2656582..2656776 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  NRZ31_RS12255 (NRZ31_12255) mutT 2656837..2657250 (-) 414 WP_010635956.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62529.93 Da        Isoelectric Point: 5.4363

>NTDB_id=716473 NRZ31_RS12225 WP_005305646.1 2651220..2652926(+) (pilB) [Aeromonas dhakensis strain BC15]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMESDQDALGMEEIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=716473 NRZ31_RS12225 WP_005305646.1 2651220..2652926(+) (pilB) [Aeromonas dhakensis strain BC15]
ATGACCTCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCCTCTTCTCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCACAGCGCAAGCCCTTTGTGACCTTTCTGATTGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCCGA
CCCGACCAATGTGTCGGCACTAGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCGAAGAGAACA
AGCTGACCGCCGCCATCAGCAAGCTGATGGAGAGCGATCAGGACGCCCTTGGCATGGAGGAGATCGACGAGTCCGAGATT
TCCGAGCTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGACCAAGTACC
GTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCAACGCCGCCGGTCAATCTGGCCAACCGCTTCTCGGCCCGA
CTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAAATGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAATACCCTGCCTACCATGTGGGGCGAGAAGATCGTGATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGCTTTGACGATCGGCAGAAGGCGCAATATCTGCGCGCGCTCTCGAAGCCTCAG
GGCATGATCCTGGTGACAGGCCCGACCGGTTCGGGCAAGACAGTGTCGCTCTACACCGGCCTGAACATTCTCAACACCAC
AGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCTGGGGTCAATCAGGTACAGGTCAACCCCAAGG
CTGGACTGACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACGTGGTGATGGTTGGTGAAATACGGGAT
CTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCCACATTGCACACCAACTCGGCGGC
CGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTAACCCTGATCATGGCTCAGC
GACTGGCACGCAAGCTGTGCGACCACTGCAAGGCCCCCGAAGTGGTGCCGGAGGCTGAACTGCTGGAGTTGGGGTTCACC
CAACAGCAACTGGCCGCAGGCTTGCGGCTGTTCAAGCCGGTTGGTTGCAAGGAGTGTTCGGGCGGCTACAAGGGACGGGT
CGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGCAGATTGCGG
CCATTGCCCAGAAGGAGGGCATGCGCACCTTGCGTACCTCTGGTCTTGAGAAGGCCCGACTGGGGGTGACCAGCCTGGCC
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K1JG90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Acinetobacter baumannii D1279779

55.088

100

0.553
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.767

99.648

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.944

0.505
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.53

92.958

0.377