Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRL00_RS19015 Genome accession   NZ_CP102327
Coordinates   4151375..4153081 (+) Length   568 a.a.
NCBI ID   WP_042049838.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC05     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4146375..4158081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRL00_RS18990 (NRL00_18990) pdhR 4147550..4148314 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NRL00_RS18995 (NRL00_18995) ampD 4148590..4149162 (-) 573 WP_017766423.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NRL00_RS19000 (NRL00_19000) - 4149286..4149756 (+) 471 WP_095591545.1 TIGR02281 family clan AA aspartic protease -
  NRL00_RS19005 (NRL00_19005) nadC 4149761..4150618 (+) 858 WP_017779054.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NRL00_RS19010 (NRL00_19010) - 4150940..4151371 (+) 432 WP_042049886.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NRL00_RS19015 (NRL00_19015) pilB 4151375..4153081 (+) 1707 WP_042049838.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NRL00_RS19020 (NRL00_19020) pilC 4153197..4154438 (+) 1242 WP_005305649.1 type II secretion system F family protein Machinery gene
  NRL00_RS19025 (NRL00_19025) pilD 4154461..4155333 (+) 873 WP_268009746.1 prepilin peptidase Machinery gene
  NRL00_RS19030 (NRL00_19030) coaE 4155353..4155967 (+) 615 WP_268009747.1 dephospho-CoA kinase -
  NRL00_RS19035 (NRL00_19035) zapD 4156005..4156727 (+) 723 WP_025202564.1 cell division protein ZapD -
  NRL00_RS19040 (NRL00_19040) yacG 4156737..4156931 (+) 195 WP_017782442.1 DNA gyrase inhibitor YacG -
  NRL00_RS19045 (NRL00_19045) mutT 4156992..4157405 (-) 414 WP_005305665.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62515.91 Da        Isoelectric Point: 5.4342

>NTDB_id=716175 NRL00_RS19015 WP_042049838.1 4151375..4153081(+) (pilB) [Aeromonas dhakensis strain BC05]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=716175 NRL00_RS19015 WP_042049838.1 4151375..4153081(+) (pilB) [Aeromonas dhakensis strain BC05]
ATGACCTCCAGTCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCTCTGCTCAGTGAGTCCGACTCACAGCGTTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAACCCTTTGTGACCTTTCTGATCGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CTGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCCGA
CCCGACCAATGTCTCAGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCGAGGAGAACA
AGCTGACCGCCGCCATCAGCAAGCTGATGGAGAGCGATCAGGACGCCCTCGGCATGGAGGACATCGACGAGTCCGAGATT
TCCGAGCTGGAGGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGACCAAGTACC
GTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTCGCGACGCCGCCGGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTAATGGCGCGTCTGGACATCGCCGAACGACGGCTACCCCAGGATGGCCGCATCAAATTGAAAATGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGCTTCGACGATCGACAGAAGGCGCAATATCTGCGTGCGCTCTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCTCAACACCAC
AGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCAGGGGTCAATCAGGTACAGGTCAACCCCAAGG
CTGGGCTGACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACGTAGTGATGGTTGGGGAAATACGGGAT
CTAGAAACCGCCGAGATCGCCATCAAGGCGGCACAAACCGGTCACCTGGTGCTTTCCACATTGCACACCAACTCGGCAGC
CGAGACGCTGACCCGGATGATGAACATGGGGGTACCCGCCTTCAACATCGCCTCCTCGGTTACTCTGATCATGGCTCAGC
GACTGGCGCGCAAGCTGTGCGACCACTGCAAGGCTCCCGAAGTGGTGCCGGAGGCTGAACTGCTGGAGTTGGGGTTTACC
CAACAGCAGCTGGCAGCAGGCTTGCGGCTGTTCAAGCCGGTTGGCTGCAAGGAGTGTTCTGGCGGCTACAAGGGACGGGT
CGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGCAGATTGCGG
CCATTGCCCAGAAGGAGGGCATGCGGACCTTGCGTACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACCAGCCTGGCC
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Acinetobacter baumannii D1279779

54.833

100

0.549
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.045

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.944

0.504
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.53

92.958

0.377