Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRK99_RS19970 Genome accession   NZ_CP102326
Coordinates   4348021..4349727 (+) Length   568 a.a.
NCBI ID   WP_017766420.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC04     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4343021..4354727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRK99_RS19945 (NRK99_19945) pdhR 4344199..4344963 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NRK99_RS19950 (NRK99_19950) ampD 4345239..4345811 (-) 573 WP_126880607.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NRK99_RS19955 (NRK99_19955) - 4345935..4346405 (+) 471 WP_010635963.1 TIGR02281 family clan AA aspartic protease -
  NRK99_RS19960 (NRK99_19960) nadC 4346410..4347267 (+) 858 WP_010635962.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NRK99_RS19965 (NRK99_19965) tapA 4347589..4348017 (+) 429 WP_081304776.1 type IVa pilus major pilin TapA -
  NRK99_RS19970 (NRK99_19970) pilB 4348021..4349727 (+) 1707 WP_017766420.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NRK99_RS19975 (NRK99_19975) pilC 4349843..4351084 (+) 1242 WP_005305649.1 type II secretion system F family protein Machinery gene
  NRK99_RS19980 (NRK99_19980) pilD 4351107..4351979 (+) 873 WP_268000069.1 prepilin peptidase Machinery gene
  NRK99_RS19985 (NRK99_19985) coaE 4351999..4352613 (+) 615 WP_126880609.1 dephospho-CoA kinase -
  NRK99_RS19990 (NRK99_19990) zapD 4352651..4353373 (+) 723 WP_026141277.1 cell division protein ZapD -
  NRK99_RS19995 (NRK99_19995) yacG 4353383..4353577 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  NRK99_RS20000 (NRK99_20000) mutT 4353638..4354051 (-) 414 WP_010635956.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62542.93 Da        Isoelectric Point: 5.4342

>NTDB_id=716147 NRK99_RS19970 WP_017766420.1 4348021..4349727(+) (pilB) [Aeromonas dhakensis strain BC04]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMENDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=716147 NRK99_RS19970 WP_017766420.1 4348021..4349727(+) (pilB) [Aeromonas dhakensis strain BC04]
ATGACCTCTAGTCCCAATAGCGGCCTGGCGTTAAGTCTGGCCGCTTCTTCCCTGCTCAGTGAGTCAGACTCACAGCGTTA
TCTGAGCCAGGCTAAGGCGCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTACTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCCGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCGAAGAGAACA
AGCTGACCGCCGCCATCAGCAAGCTGATGGAGAACGATCAGGACGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTGGAGGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCCCATCGTCAA
ATACATAAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGACCAAGTACC
GTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGACGGCTGCCCCAGGATGGCCGCATCAAATTGAAGATGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGCTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCGCTCTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCTCAACACCAC
AGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAAATCAACCTGCCTGGGGTCAATCAGGTGCAGGTCAACCCCAAGG
CTGGGCTGACCTTCGCCAGTGCCCTGCGCTCCTTTCTGCGCCAGGATCCCGATGTGGTGATGGTCGGGGAAATACGGGAT
CTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCCACATTGCACACCAACTCGGCGGC
CGAGACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCATGGCCCAGC
GACTGGCACGCAAGCTGTGTGACCACTGCAAGGCTCCCGAAGTGGTGCCGGAAGCTGAACTGCTGGAGTTGGGGTTCACC
CAACAGCAGCTGGCAGCAGGCTTGCGGCTGTTCAAGCCGGTCGGTTGCAAGGAGTGTTCGGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAATTCCATGCAGATTGCGG
CCATTGCCCAGAAGGAGGGCATGCGGACCTTGCGCACCTCCGGTCTTGAAAAGGCCCGGCTGGGGGTGACCAGCCTGGCC
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Acinetobacter baumannii D1279779

54.833

100

0.549
  pilB Legionella pneumophila strain ERS1305867

54.417

99.648

0.542
  pilB Vibrio cholerae strain A1552

53.805

99.472

0.535
  pilB Vibrio campbellii strain DS40M4

51.59

99.648

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.944

0.504
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.53

92.958

0.377