Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRK98_RS20945 Genome accession   NZ_CP102325
Coordinates   4600649..4602364 (-) Length   571 a.a.
NCBI ID   WP_219326273.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC03     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4595649..4607364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRK98_RS20915 (NRK98_20915) mutT 4596325..4596738 (+) 414 WP_010635956.1 8-oxo-dGTP diphosphatase MutT -
  NRK98_RS20920 (NRK98_20920) yacG 4596799..4596993 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  NRK98_RS20925 (NRK98_20925) zapD 4597003..4597725 (-) 723 WP_268027961.1 cell division protein ZapD -
  NRK98_RS20930 (NRK98_20930) coaE 4597763..4598377 (-) 615 WP_017766418.1 dephospho-CoA kinase -
  NRK98_RS20935 (NRK98_20935) pilD 4598397..4599269 (-) 873 WP_268027962.1 prepilin peptidase Machinery gene
  NRK98_RS20940 (NRK98_20940) pilC 4599292..4600533 (-) 1242 WP_005305649.1 type II secretion system F family protein Machinery gene
  NRK98_RS20945 (NRK98_20945) pilB 4600649..4602364 (-) 1716 WP_219326273.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NRK98_RS20950 (NRK98_20950) - 4602355..4602795 (-) 441 WP_082969962.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NRK98_RS20955 (NRK98_20955) nadC 4603117..4603974 (-) 858 WP_268027963.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NRK98_RS20960 (NRK98_20960) - 4603979..4604449 (-) 471 WP_043171614.1 TIGR02281 family clan AA aspartic protease -
  NRK98_RS20965 (NRK98_20965) ampD 4604573..4605145 (+) 573 WP_208200766.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NRK98_RS20970 (NRK98_20970) pdhR 4605421..4606185 (+) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62884.40 Da        Isoelectric Point: 5.4342

>NTDB_id=716125 NRK98_RS20945 WP_219326273.1 4600649..4602364(-) (pilB) [Aeromonas dhakensis strain BC03]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMENDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=716125 NRK98_RS20945 WP_219326273.1 4600649..4602364(-) (pilB) [Aeromonas dhakensis strain BC03]
CTGCCCATAATGACATCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAGGCCAAGGCGCAGCGCAAACCCTTTGTGACCTTTCTGATCGAGAACGAGATCCTCGACAGCA
AGGCGCTGGCCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGACACACCCTCTATATCGC
CATGTCCGACCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCG
AAGAGAACAAGCTGACCGCCGCCATCAGCAAGCTGATGGAGAACGATCAGGACGCCCTCGGCATGGAGGACATCGACGAG
TCCGAGATCTCCGAGCTGGAGGTGTCTGACGAAGGGTCACGACTCGACGAGAGCGTCAACACTGCGGATGATGATGCCCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGA
CCAAGTACCGTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTC
TCGGCCCGACTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAAAT
GTCGCGCAACAAGTCGATGGACATGCGGGTGAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCG
ACTCTTCCGCTGCGCGCCTCAATATCGAACAGCTCGGCTTTGACGATCGGCAGAAGGCACAATATCTGCGCGCGCTCTCG
AAGCCTCAGGGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGTAAGACGGTGTCGCTCTACACCGGCCTGAACATTCT
CAACACCACAGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCTGGGGTCAATCAGGTACAGGTCA
ACCCCAAGGCCGGCCTGACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCAGATGTGGTGATGGTCGGGGAA
ATACGGGATCTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCTACATTGCACACCAA
CTCGGCGGCCGAGACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCA
TGGCTCAGCGACTGGCACGCAAGCTCTGTGACCACTGCAAGGCTCCCGAAGTGGTGCCGGAAGCTGAACTGCTGGAGTTG
GGGTTCACCCAACAGCAGCTGGCCGCCGGCTTGCGGCTGTTCAAGCCGGTCGGCTGCAAGGAGTGTTCTGGCGGCTACAA
GGGACGGGTCGGCATCTACGAAATCCTGTTGATGTCCGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGC
AGATTGCGGCTATCGCCCAGAAGGAGGGCATGCGCACCTTGCGGACCTCCGGTCTTGAGAAAGCCCGACTGGGGGTGACC
AGCCTGGCCGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.148

100

0.553
  pilB Acinetobacter baumannii D1279779

54.53

100

0.548
  pilB Legionella pneumophila strain ERS1305867

54.417

99.124

0.539
  pilB Vibrio cholerae strain A1552

53.805

98.949

0.532
  pilB Vibrio campbellii strain DS40M4

51.59

99.124

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.424

0.501
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

96.848

0.375
  pilF Thermus thermophilus HB27

40.53

92.469

0.375