Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRK98_RS05015 Genome accession   NZ_CP102325
Coordinates   1081492..1082631 (-) Length   379 a.a.
NCBI ID   WP_026141098.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1076492..1087631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRK98_RS04995 (NRK98_04995) feoB 1076687..1078960 (+) 2274 WP_268028431.1 Fe(2+) transporter permease subunit FeoB -
  NRK98_RS05000 (NRK98_05000) - 1078957..1079199 (+) 243 WP_005302637.1 FeoC-like transcriptional regulator -
  NRK98_RS05005 (NRK98_05005) - 1079323..1079802 (-) 480 WP_268028432.1 WbuC family cupin fold metalloprotein -
  NRK98_RS05010 (NRK98_05010) glgC 1079938..1081209 (-) 1272 WP_005302634.1 glucose-1-phosphate adenylyltransferase -
  NRK98_RS05015 (NRK98_05015) pilU 1081492..1082631 (-) 1140 WP_026141098.1 type IVa pilus ATPase TapW Machinery gene
  NRK98_RS05020 (NRK98_05020) - 1082691..1083047 (-) 357 WP_005302630.1 DMT family protein -
  NRK98_RS05025 (NRK98_05025) - 1083161..1083892 (+) 732 WP_005302626.1 substrate-binding periplasmic protein -
  NRK98_RS05030 (NRK98_05030) btuC 1083992..1084999 (+) 1008 WP_219325485.1 vitamin B12 ABC transporter permease BtuC -
  NRK98_RS05035 (NRK98_05035) - 1084986..1085771 (+) 786 WP_054544543.1 ATP-binding cassette domain-containing protein -
  NRK98_RS05040 (NRK98_05040) - 1085851..1086912 (-) 1062 WP_017766699.1 3-deoxy-7-phosphoheptulonate synthase -
  NRK98_RS05045 (NRK98_05045) - 1087069..1087449 (+) 381 WP_005302618.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42185.21 Da        Isoelectric Point: 6.2566

>NTDB_id=716106 NRK98_RS05015 WP_026141098.1 1081492..1082631(-) (pilU) [Aeromonas dhakensis strain BC03]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=716106 NRK98_RS05015 WP_026141098.1 1081492..1082631(-) (pilU) [Aeromonas dhakensis strain BC03]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAACAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGACTTGAAACTGCCCACAGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGTT
CGGGCAAATCCACCTCGCTCGCAGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATCGGCGAAATTCGCGATCGCGAAACCATGGAGCACG
CGCTGGCCTTCTCGGAAACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGAGTGATCGAGGAGGAAGAGGCAGTGAAGAACGCCGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

89.446

0.369
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361