Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NQ200_RS18670 Genome accession   NZ_CP102172
Coordinates   4097808..4099514 (+) Length   568 a.a.
NCBI ID   WP_096119753.1    Uniprot ID   -
Organism   Aeromonas salmonicida subsp. masoucida strain wx3-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4092808..4104514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ200_RS18645 pdhR 4093945..4094709 (-) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NQ200_RS18650 ampD 4094986..4095558 (-) 573 WP_113720816.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NQ200_RS18655 - 4095708..4096178 (+) 471 WP_309265796.1 retropepsin-like aspartic protease -
  NQ200_RS18660 nadC 4096183..4097040 (+) 858 WP_282683318.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NQ200_RS18665 - 4097376..4097801 (+) 426 WP_309265797.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NQ200_RS18670 pilB 4097808..4099514 (+) 1707 WP_096119753.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NQ200_RS18675 pilC 4099643..4100884 (+) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  NQ200_RS18680 pilD 4100948..4101823 (+) 876 WP_125729778.1 A24 family peptidase Machinery gene
  NQ200_RS18685 coaE 4101843..4102457 (+) 615 WP_309265798.1 dephospho-CoA kinase -
  NQ200_RS18690 zapD 4102495..4103217 (+) 723 WP_005314166.1 cell division protein ZapD -
  NQ200_RS18695 yacG 4103227..4103421 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  NQ200_RS18700 mutT 4103499..4103906 (-) 408 WP_005314168.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62575.03 Da        Isoelectric Point: 5.4384

>NTDB_id=715154 NQ200_RS18670 WP_096119753.1 4097808..4099514(+) (pilB) [Aeromonas salmonicida subsp. masoucida strain wx3-1]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTSAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTIEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=715154 NQ200_RS18670 WP_096119753.1 4097808..4099514(+) (pilB) [Aeromonas salmonicida subsp. masoucida strain wx3-1]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCTCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTACTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCTATGTCGGA
TCCGACCAACGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCTCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAACTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCAGATGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAAATAGCGACGCCGCCAGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGACTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGTGCCCTCTCCAAGCCCCAG
GGCATGATATTGGTGACAGGCCCGACCGGCTCTGGCAAAACAGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCAT
AGAGGTCAACATCTCTACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTTCTTCGCCAGGATCCCGATGTGGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGCATGATGAACATGGGGGTCCCGGCCTTCAATATTGCCTCTTCGGTGACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCACCGGAAGTGGTGCCCGAAGCTGAATTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAACCGGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGGGCCAACTCCCTGCAAATTGCAG
CGATTGCTCAAAAAGAGGGAATGCGAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.556

99.824

0.555
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373