Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NQU90_RS01915 Genome accession   NZ_CP102108
Coordinates   392859..394565 (-) Length   568 a.a.
NCBI ID   WP_257124213.1    Uniprot ID   -
Organism   Aeromonas veronii strain GD21SC2322TT     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 387859..399565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQU90_RS01885 (NQU90_01885) mutT 388371..388775 (+) 405 WP_257124211.1 8-oxo-dGTP diphosphatase MutT -
  NQU90_RS01890 (NQU90_01890) yacG 388851..389045 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  NQU90_RS01895 (NQU90_01895) zapD 389055..389777 (-) 723 WP_216952203.1 cell division protein ZapD -
  NQU90_RS01900 (NQU90_01900) coaE 389815..390429 (-) 615 WP_257124212.1 dephospho-CoA kinase -
  NQU90_RS01905 (NQU90_01905) pilD 390443..391315 (-) 873 WP_236356080.1 A24 family peptidase Machinery gene
  NQU90_RS01910 (NQU90_01910) pilC 391377..392618 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  NQU90_RS01915 (NQU90_01915) pilB 392859..394565 (-) 1707 WP_257124213.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NQU90_RS01920 (NQU90_01920) - 394565..394942 (-) 378 WP_151286536.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NQU90_RS01925 (NQU90_01925) nadC 395258..396133 (-) 876 WP_257124214.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NQU90_RS01930 (NQU90_01930) - 396137..396607 (-) 471 WP_005336129.1 TIGR02281 family clan AA aspartic protease -
  NQU90_RS01935 (NQU90_01935) ampD 396738..397313 (+) 576 WP_019444780.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NQU90_RS01940 (NQU90_01940) pdhR 397592..398356 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62547.90 Da        Isoelectric Point: 5.3192

>NTDB_id=714449 NQU90_RS01915 WP_257124213.1 392859..394565(-) (pilB) [Aeromonas veronii strain GD21SC2322TT]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGITSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=714449 NQU90_RS01915 WP_257124213.1 392859..394565(-) (pilB) [Aeromonas veronii strain GD21SC2322TT]
ATGACCTCCAGTCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGCGAAGAGGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCTCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAGCACCACGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCAGCGTTGGAGGACTTTGGCTTCAGCTTCGGTCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAAAGTGTCAATACCGCCGATGACGATGCCCCCATTGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTCCGCTTTCGCATCGACGGCATTCTGCATGAAATCGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCGCGGCTCGATATTGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTTAATACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGACAGAAGGCGCAGTACCTGCGCGCCCTTTCGAAACCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACAGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTGAATATCTCCACCGCAGAAGACCCGGTAGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGATCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTCGAAACCGCCGAAATCGCTATCAAGGCGGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
AGAGACCCTGACCCGGATGATGAACATGGGGGTACCGGCCTTCAACATCGCCTCCTCGGTCACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCACCGGAAGTGGTACCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCGGCCGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGAGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCAGAGAACATCGCCAAATTGATCATGCAGGGGGCAAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAAGAGGGGATGCGTACCCTGCGCACCTCGGGGCTGGAGAAGGCCCGGCTCGGGATCACCAGTCTGGCC
GAGGTCAATCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

100

0.558
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

54.937

98.063

0.539
  pilB Vibrio campbellii strain DS40M4

51.394

100

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.773

99.296

0.514
  pilF Neisseria gonorrhoeae MS11

46.986

99.296

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.72

92.958

0.379