Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NQU59_RS01685 Genome accession   NZ_CP102099
Coordinates   353758..355470 (+) Length   570 a.a.
NCBI ID   WP_005240082.1    Uniprot ID   -
Organism   Acinetobacter colistiniresistens strain C-214     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 348758..360470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQU59_RS01650 (NQU59_01650) nusA 349581..351065 (-) 1485 WP_004656210.1 transcription termination factor NusA -
  NQU59_RS01655 (NQU59_01655) rimP 351103..351627 (-) 525 WP_005240078.1 ribosome maturation factor RimP -
  NQU59_RS01675 (NQU59_01675) secG 352254..352583 (-) 330 WP_004656213.1 preprotein translocase subunit SecG -
  NQU59_RS01680 (NQU59_01680) tpiA 352596..353387 (-) 792 WP_096911157.1 triose-phosphate isomerase -
  NQU59_RS01685 (NQU59_01685) pilB 353758..355470 (+) 1713 WP_005240082.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NQU59_RS01690 (NQU59_01690) pilC 355495..356721 (+) 1227 WP_096911156.1 type II secretion system F family protein Machinery gene
  NQU59_RS01695 (NQU59_01695) pilD 356727..357587 (+) 861 WP_005240087.1 A24 family peptidase Machinery gene
  NQU59_RS01700 (NQU59_01700) coaE 357589..358194 (+) 606 WP_257064720.1 dephospho-CoA kinase -
  NQU59_RS01705 (NQU59_01705) - 358191..359102 (-) 912 WP_257064722.1 DMT family transporter -
  NQU59_RS01710 (NQU59_01710) rlmB 359133..359882 (-) 750 WP_005278530.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  NQU59_RS01715 (NQU59_01715) - 360081..360407 (-) 327 WP_004637677.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63095.40 Da        Isoelectric Point: 4.9551

>NTDB_id=714377 NQU59_RS01685 WP_005240082.1 353758..355470(+) (pilB) [Acinetobacter colistiniresistens strain C-214]
MSGLHTSPKFSGFIRRLVEDGQLSAATMQSAIDAAKKAQQDIVAHLIEQHRLSPLTIAETIAAEFGEPLFDLAVYDTALL
PRELADNKLIQKHKVAPLLQRGQILYVATSNPTNIEALDAIRFNTKLNIEPIIVEHTKLEKLIEQQFGDSGSFDFGESED
FDLDIDVSQAPTQEEEDAPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQMATRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMDALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCGQCKEVIEIPKQSLLELGF
TEQDLNDPEFKIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEQAGFNNLRRSGLKKVMQGITS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=714377 NQU59_RS01685 WP_005240082.1 353758..355470(+) (pilB) [Acinetobacter colistiniresistens strain C-214]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTTATTCGGCGCTTAGTTGAAGATGGGCAACTGTCTGCAGCGAC
CATGCAAAGCGCAATTGATGCTGCTAAAAAAGCACAACAAGACATCGTCGCACATTTGATTGAGCAACATCGCTTATCAC
CACTCACAATTGCTGAAACCATTGCAGCCGAGTTTGGTGAGCCTCTTTTCGATTTGGCAGTCTACGATACAGCGCTATTA
CCTCGCGAGCTGGCAGATAATAAGCTCATTCAAAAGCATAAAGTTGCACCGCTATTGCAACGCGGACAAATTCTGTATGT
TGCAACCAGTAATCCCACCAATATTGAAGCCTTGGATGCCATTCGTTTCAATACCAAGCTCAATATTGAACCGATTATTG
TTGAACACACCAAACTCGAAAAACTGATCGAACAGCAGTTTGGTGATTCTGGCAGCTTTGATTTTGGCGAAAGCGAAGAC
TTTGACCTTGATATCGATGTTAGCCAAGCTCCCACCCAAGAGGAGGAGGATGCTCCTCAAGGTGACGAATCCCCTATTGT
AAAATACATCAACAAACTGCTAATTGATGCGATTCGTATGGGTGCTTCTGATTTACATTTTGAACCCTATGAAAAAATGT
ATCGTGTCCGTTATCGCGTTGATGGGGTATTACGTCAGATTGCCAATCCACCACTACAAATGGCAACCCGATTGGCATCA
CGTTTAAAAGTGATGTCGCAAATGGACATTTCAGAAAAACGAATGCCTCAAGATGGCCGTATCAAACTCAAACTCTCCAA
GACCAAAGCCATCGATTTCCGTGTTAACTCACTCCCGACACTATTTGGTGAGAAACTGGTGCTGCGTATTCTCGATCCGT
CGAGCGCGATGCTCGGTATTGAAGCTTTGGGCTATGAAGATGAACAAAAAGCCTTGTTCATGGATGCTCTGGATAAACCT
CAAGGCATGCTCCTCATCACAGGTCCAACCGGTTCAGGTAAAACCGTTTCCTTATATACAGGTTTAAATATCCTCAATAC
TGAGGATACCAACATTTCGACCGCCGAAGATCCGGTCGAAATCAACTTGGAAGGGATCAATCAGGTCAATGTGAATCCCA
AAACTGGACTCACCTTCTCGGCTGCACTTAAAGCCTTTTTACGCCAAGATCCAGATGTGATTATGGTCGGGGAGATTCGT
GATCTGGAAACAGCAGAAATTGCAATTAAGGCTGCTCAAACAGGCCATATGGTCATGTCGACGCTGCATACCAACAGCGC
ACCAGAAACCTTGACGCGTTTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACGTCAGTCAATTTGGTAATTGCAC
AACGTTTGGCACGTCGCCTCTGTGGGCAATGCAAAGAAGTAATTGAAATTCCTAAACAAAGCTTACTAGAACTCGGCTTT
ACTGAACAAGATTTAAATGATCCTGAGTTTAAAATTTTTCAACCTGTGGGATGTTCTGAATGCCGTGAAGGCTACAAAGG
CCGAGTCGGTATTTATGAAGTCATGAAAGTCACGCCAGAGATTTCTAAAATTATTATGGAAGATGGCAATGCCTTACAAA
TTGCGGCAGCATCAGAACAAGCAGGCTTTAATAATCTACGTCGTTCAGGTTTGAAAAAAGTCATGCAGGGAATTACCTCT
TTGCAGGAAGTCAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

83.187

100

0.833
  pilB Acinetobacter baylyi ADP1

76.182

100

0.763
  pilB Legionella pneumophila strain ERS1305867

53.506

95.088

0.509
  pilF Neisseria gonorrhoeae MS11

48.592

99.649

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

97.544

0.477
  pilB Vibrio cholerae strain A1552

49.094

96.842

0.475
  pilB Vibrio campbellii strain DS40M4

51.158

90.877

0.465
  pilF Thermus thermophilus HB27

37.456

99.298

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

33.55

100

0.361