Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NQD60_RS00990 Genome accession   NZ_CP102085
Coordinates   235415..236686 (-) Length   423 a.a.
NCBI ID   WP_257101220.1    Uniprot ID   -
Organism   Mycoavidus sp. SF9855     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 230415..241686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQD60_RS00960 (NQD60_00960) - 230682..231119 (+) 438 WP_257101219.1 NUDIX domain-containing protein -
  NQD60_RS00965 (NQD60_00965) - 231157..231345 (-) 189 WP_257100713.1 DNA gyrase inhibitor YacG -
  NQD60_RS00970 (NQD60_00970) zapD 231444..232202 (-) 759 WP_257100714.1 cell division protein ZapD -
  NQD60_RS00975 (NQD60_00975) coaE 232381..233004 (-) 624 WP_223693238.1 dephospho-CoA kinase -
  NQD60_RS00980 (NQD60_00980) - 233131..234126 (-) 996 WP_257100715.1 A24 family peptidase -
  NQD60_RS00985 (NQD60_00985) - 234180..235406 (-) 1227 WP_257100716.1 type II secretion system F family protein -
  NQD60_RS00990 (NQD60_00990) pilF 235415..236686 (-) 1272 WP_257101220.1 GspE/PulE family protein Machinery gene
  NQD60_RS00995 (NQD60_00995) - 236914..238209 (-) 1296 WP_257100717.1 HlyC/CorC family transporter -
  NQD60_RS01000 (NQD60_01000) - 238263..239192 (-) 930 WP_257101221.1 polyprenyl synthetase family protein -
  NQD60_RS01005 (NQD60_01005) - 239343..240152 (-) 810 WP_257100718.1 cytochrome c oxidase assembly protein -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45754.77 Da        Isoelectric Point: 6.9076

>NTDB_id=714175 NQD60_RS00990 WP_257101220.1 235415..236686(-) (pilF) [Mycoavidus sp. SF9855]
MNTLADSATAVPPPNALSSTGPKEDDNAPAVRLLRELLLDAATHGASDVHIEPSEHDWRIRLRIDGVLHETMRPPPYLRD
PFICCVKVLARLDIAERRIPQDGRLKLSVAADKFEEFRVNTLPTLWGEKLVLRRLDTLPATLTLTDLGFDLKQQATLAAA
IHAPHGMVLVTGPTGSGKTLSLYCFLQHLNRESINICSVEDPAEIQLAGVNQVSVRDKAGLTFAVALRAFLRQDPDVIMV
GEIRDTETASVALQAAQTGHLVLSTLHTNDAPATLARLLDMGIAPFNLACAMRLIIAQRLVRKLCLACRAPAFTHQTTAE
AMLRSAGFDETTLAEDWQPYRAVGCAACHGIGYRGRIGIHQVMPVTAAIRGLIMARSSTDAIAQQALREGVKSLREAGLA
QVRAGTTSLEEILAVTDASPATF

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=714175 NQD60_RS00990 WP_257101220.1 235415..236686(-) (pilF) [Mycoavidus sp. SF9855]
ATGAATACGCTTGCGGATTCAGCAACTGCGGTACCACCTCCGAACGCCTTATCCTCTACTGGACCCAAAGAGGACGATAA
TGCTCCAGCTGTGCGACTATTGCGTGAACTTTTACTTGATGCTGCCACCCATGGCGCATCTGACGTGCATATTGAGCCTA
GCGAGCATGATTGGCGCATTCGGCTACGCATCGATGGGGTGCTGCATGAAACCATGCGCCCGCCGCCCTATCTAAGAGAT
CCGTTCATCTGTTGCGTGAAAGTGCTAGCTCGCCTCGATATTGCCGAGCGGCGCATACCTCAAGACGGACGCCTCAAACT
ATCCGTTGCAGCAGACAAATTTGAAGAATTCCGAGTTAATACACTACCCACCTTGTGGGGCGAAAAACTCGTTTTGCGGC
GACTTGACACCCTCCCAGCCACGCTTACTCTGACTGACCTCGGTTTTGACCTCAAACAACAAGCCACTCTGGCCGCAGCT
ATTCACGCCCCGCACGGCATGGTATTGGTTACAGGCCCAACGGGGAGCGGCAAAACTTTATCGTTATATTGCTTTTTACA
GCACTTAAATCGAGAATCAATCAATATCTGCTCGGTCGAAGACCCAGCTGAAATACAATTAGCAGGCGTTAATCAGGTCA
GTGTACGCGACAAAGCGGGCCTTACCTTTGCCGTCGCACTGCGCGCCTTTTTACGACAAGATCCTGATGTGATTATGGTT
GGAGAAATTCGTGACACTGAAACCGCCAGTGTTGCACTACAAGCCGCACAAACGGGTCACCTTGTCTTATCCACCCTGCA
TACCAATGATGCTCCCGCCACACTCGCACGCTTACTTGATATGGGCATAGCGCCTTTTAACTTGGCTTGCGCCATGCGCT
TAATTATCGCGCAAAGACTGGTCAGAAAACTCTGCTTAGCCTGTCGCGCCCCAGCTTTCACCCATCAGACAACTGCTGAG
GCGATGCTGCGTAGCGCGGGCTTTGACGAAACAACCCTAGCAGAAGATTGGCAACCCTATCGAGCCGTCGGCTGCGCTGC
GTGTCATGGCATTGGTTATCGCGGCCGCATTGGCATCCATCAAGTGATGCCGGTTACCGCTGCGATACGCGGGCTCATCA
TGGCACGAAGCAGCACCGATGCAATCGCTCAACAAGCGCTGCGTGAAGGCGTAAAAAGCTTACGAGAGGCAGGCTTAGCG
CAAGTACGCGCCGGCACAACCAGCCTCGAAGAAATTCTAGCCGTCACAGATGCCAGCCCGGCTACTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

52.525

93.617

0.492
  pilB Acinetobacter baumannii D1279779

51.02

92.671

0.473
  pilB Acinetobacter baylyi ADP1

51.026

92.199

0.47
  pilB Legionella pneumophila strain ERS1305867

50.382

92.908

0.468
  pilB Vibrio cholerae strain A1552

48.762

95.508

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

47.704

92.671

0.442
  pilB Vibrio campbellii strain DS40M4

47.449

92.671

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

42.548

98.345

0.418
  pilF Thermus thermophilus HB27

43.038

93.381

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.748

92.908

0.397
  pilB Haemophilus influenzae Rd KW20

40.602

94.326

0.383
  pilB Haemophilus influenzae 86-028NP

40.1

94.326

0.378
  pilB Glaesserella parasuis strain SC1401

45.455

80.615

0.366