Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J59_RS03755 Genome accession   NZ_AP018782
Coordinates   749491..750885 (+) Length   464 a.a.
NCBI ID   WP_005666685.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-V-2 isolate 13H37     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 744491..755885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J59_RS03730 (CHBNV2_07060) rsmE 744509..745246 (-) 738 WP_005666674.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J59_RS03735 (CHBNV2_07070) lnt 745299..746825 (-) 1527 WP_020909963.1 apolipoprotein N-acyltransferase -
  K6J59_RS03740 (CHBNV2_07080) corC 746848..747747 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J59_RS03745 (CHBNV2_07090) ampD 748379..748930 (-) 552 WP_005666680.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J59_RS03750 (CHBNV2_07100) pilA 749045..749494 (+) 450 WP_005666683.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J59_RS03755 (CHBNV2_07110) pilB 749491..750885 (+) 1395 WP_005666685.1 GspE/PulE family protein Machinery gene
  K6J59_RS03760 (CHBNV2_07120) pilC 750882..752102 (+) 1221 WP_005666687.1 type II secretion system F family protein Machinery gene
  K6J59_RS03765 (CHBNV2_07130) pilD 752099..752791 (+) 693 WP_005666688.1 A24 family peptidase Machinery gene
  K6J59_RS03770 (CHBNV2_07140) rho 752846..754108 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  K6J59_RS03775 (CHBNV2_07150) metJ 754355..754672 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J59_RS03780 (CHBNV2_07160) cueR 754686..755072 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J59_RS03785 (CHBNV2_07170) - 755149..755355 (+) 207 WP_005666692.1 heavy-metal-associated domain-containing protein -
  K6J59_RS03790 (CHBNV2_07180) - 755441..755647 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52836.39 Da        Isoelectric Point: 6.1942

>NTDB_id=71359 K6J59_RS03755 WP_005666685.1 749491..750885(+) (pilB) [Haemophilus influenzae strain CHBN-V-2 isolate 13H37]
MTSYALLHTQHVTAKNGEVFTISPELWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQKLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIVQSQINPQIGLDFNRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIESSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDEKEIERVLGKKS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=71359 K6J59_RS03755 WP_005666685.1 749491..750885(+) (pilB) [Haemophilus influenzae strain CHBN-V-2 isolate 13H37]
ATGACGAGCTATGCTTTACTTCATACTCAGCATGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGAGTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTTTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTAAAAGAACTGTTACAGAAACTTACTCCGTGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCCGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCTTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTTACAGGTCCCACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACAGCGGAAGATC
CCATTGAAATTGAGCTGGATGGCATTGTTCAAAGCCAAATTAATCCACAGATTGGACTAGATTTTAACCGCCTATTGCGT
ACTTTCTTACGCCAAGATCCCGATATTATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTGCGTGC
TGCACAAACTGGGCATCTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATATCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAGCAGTTTACTGCTCGTCATTGCGCAGCGTCTTGTACGAAAAATCTGCCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGCTAGGTAAAAAGTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(23-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

96.97

99.569

0.966
  pilB Haemophilus influenzae 86-028NP

96.336

100

0.963
  pilB Glaesserella parasuis strain SC1401

57.237

98.276

0.563
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Legionella pneumophila strain ERS1305867

39.148

100

0.416
  pilB Acinetobacter baylyi ADP1

38.105

100

0.407
  pilB Acinetobacter baumannii D1279779

38.351

100

0.401
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.179

100

0.375
  pilF Thermus thermophilus HB27

35.714

100

0.366

Multiple sequence alignment