Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NP431_RS12080 Genome accession   NZ_CP101905
Coordinates   2654323..2654781 (+) Length   152 a.a.
NCBI ID   WP_020333947.1    Uniprot ID   A0AAN0Y3X9
Organism   Vibrio natriegens strain 14048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2649323..2659781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP431_RS12065 (NP431_12065) pdhR 2651354..2652121 (-) 768 WP_014232947.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NP431_RS12070 (NP431_12070) ampD 2652531..2653076 (-) 546 WP_020333945.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NP431_RS12075 (NP431_12075) nadC 2653169..2654056 (+) 888 WP_020333946.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NP431_RS12080 (NP431_12080) pilA 2654323..2654781 (+) 459 WP_020333947.1 pilin Machinery gene
  NP431_RS12085 (NP431_12085) pilB 2654782..2656467 (+) 1686 WP_020333948.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NP431_RS12090 (NP431_12090) pilC 2656498..2657721 (+) 1224 WP_020333949.1 type II secretion system F family protein Machinery gene
  NP431_RS12095 (NP431_12095) pilD 2657789..2658658 (+) 870 WP_020333950.1 A24 family peptidase Machinery gene
  NP431_RS12100 (NP431_12100) coaE 2658659..2659273 (+) 615 WP_020333951.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15920.27 Da        Isoelectric Point: 7.7804

>NTDB_id=712638 NP431_RS12080 WP_020333947.1 2654323..2654781(+) (pilA) [Vibrio natriegens strain 14048]
MKSNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSALGVGLANITALKTNIEDYIATEGSFPSTTSGTKADFT
RLGTVEDMGDGNVLITPTSDGSLDGTVKFTFDAGVVNTQKIQLVRSGGLWICETTAASEISPKGCAAGSTIK

Nucleotide


Download         Length: 459 bp        

>NTDB_id=712638 NP431_RS12080 WP_020333947.1 2654323..2654781(+) (pilA) [Vibrio natriegens strain 14048]
ATGAAGTCAAATAAACAGAAAAAACAGCAAGGCTTTACGCTGATTGAATTAATGATCGTGGTGGCGGTAATAGGGGTTTT
GGCAGCAATTGCTATCCCTCAGTACCAAAACTACGTAAAGAAATCAGCTTTAGGAGTAGGCCTAGCAAATATTACTGCTC
TGAAAACTAACATCGAGGATTATATTGCTACTGAAGGTAGCTTTCCTTCAACAACATCAGGTACCAAAGCTGATTTTACG
CGGCTCGGTACCGTAGAAGACATGGGAGATGGCAACGTTCTAATCACCCCTACAAGCGACGGCTCTTTAGATGGAACAGT
AAAATTCACCTTTGATGCAGGTGTCGTCAATACACAAAAGATTCAGTTAGTTCGATCTGGTGGACTTTGGATATGTGAAA
CGACAGCAGCAAGTGAAATATCTCCAAAAGGTTGTGCTGCTGGTTCAACGATTAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

77.632

100

0.776
  pilA Vibrio cholerae C6706

77.632

100

0.776
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

77.632

100

0.776
  pilA Pseudomonas aeruginosa PAK

43.226

100

0.441
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

97.368

0.414
  pilA Acinetobacter baumannii strain A118

40.397

99.342

0.401
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.86

94.079

0.375