Detailed information NTG0712592

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NPS25_RS12970
• Genome accession: NZ_CP101904
• Coordinates: 2666693..2667307 (-)
• Length: 204 a.a.
• NCBI ID: WP_015417854.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Ba-0321
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2661693..2672307

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NPS25_RS12940 (NPS25_12940)	rpsT	2661996..2662262 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
NPS25_RS12945 (NPS25_12945)	holA	2662279..2663320 (-)	1042	Protein_2502	DNA polymerase III subunit delta	-
NPS25_RS12950 (NPS25_12950)	-	2663360..2663511 (-)	152	Protein_2503	hypothetical protein	-
NPS25_RS12955 (NPS25_12955)	-	2663552..2663686 (+)	135	WP_003152870.1	YqzM family protein	-
NPS25_RS12960 (NPS25_12960)	comEC	2663705..2666056 (-)	2352	WP_256683591.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NPS25_RS12965 (NPS25_12965)	-	2666057..2666626 (-)	570	WP_003152868.1	ComE operon protein 2	-
NPS25_RS12970 (NPS25_12970)	comEA	2666693..2667307 (-)	615	WP_015417854.1	helix-hairpin-helix domain-containing protein	Machinery gene
NPS25_RS12975 (NPS25_12975)	comER	2667366..2668187 (+)	822	WP_015417855.1	late competence protein ComER	-
NPS25_RS12980 (NPS25_12980)	-	2668256..2668993 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
NPS25_RS12985 (NPS25_12985)	rsfS	2668990..2669346 (-)	357	WP_007408260.1	ribosome silencing factor	-
NPS25_RS12990 (NPS25_12990)	yqeK	2669364..2669924 (-)	561	WP_007408259.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NPS25_RS12995 (NPS25_12995)	-	2669914..2670483 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
NPS25_RS13000 (NPS25_13000)	yhbY	2670494..2670784 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
NPS25_RS13005 (NPS25_13005)	aroE	2670778..2671620 (-)	843	WP_033574150.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22207.39 Da
• Isoelectric Point: 6.4888

>NTDB_id=712592 NPS25_RS12970 WP_015417854.1 2666693..2667307(-) (comEA) [Bacillus velezensis strain Ba-0321]
MTKAMMTERLRRNLKILVACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=712592 NPS25_RS12970 WP_015417854.1 2666693..2667307(-) (comEA) [Bacillus velezensis strain Ba-0321]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.653	96.078	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA	Lactococcus lactis subsp. cremoris KW2	34.685	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae R6	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368