Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   NPS25_RS12960 Genome accession   NZ_CP101904
Coordinates   2663705..2666056 (-) Length   783 a.a.
NCBI ID   WP_256683591.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ba-0321     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2658705..2671056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPS25_RS12925 (NPS25_12925) - 2659071..2659409 (-) 339 WP_007408270.1 YqxA family protein -
  NPS25_RS12930 (NPS25_12930) spoIIP 2659426..2660619 (-) 1194 WP_007612673.1 stage II sporulation protein P -
  NPS25_RS12935 (NPS25_12935) gpr 2660687..2661793 (-) 1107 WP_007408268.1 GPR endopeptidase -
  NPS25_RS12940 (NPS25_12940) rpsT 2661996..2662262 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  NPS25_RS12945 (NPS25_12945) holA 2662279..2663320 (-) 1042 Protein_2502 DNA polymerase III subunit delta -
  NPS25_RS12950 (NPS25_12950) - 2663360..2663511 (-) 152 Protein_2503 hypothetical protein -
  NPS25_RS12955 (NPS25_12955) - 2663552..2663686 (+) 135 WP_003152870.1 YqzM family protein -
  NPS25_RS12960 (NPS25_12960) comEC 2663705..2666056 (-) 2352 WP_256683591.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NPS25_RS12965 (NPS25_12965) - 2666057..2666626 (-) 570 WP_003152868.1 ComE operon protein 2 -
  NPS25_RS12970 (NPS25_12970) comEA 2666693..2667307 (-) 615 WP_015417854.1 helix-hairpin-helix domain-containing protein Machinery gene
  NPS25_RS12975 (NPS25_12975) comER 2667366..2668187 (+) 822 WP_015417855.1 late competence protein ComER -
  NPS25_RS12980 (NPS25_12980) - 2668256..2668993 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  NPS25_RS12985 (NPS25_12985) rsfS 2668990..2669346 (-) 357 WP_007408260.1 ribosome silencing factor -
  NPS25_RS12990 (NPS25_12990) yqeK 2669364..2669924 (-) 561 WP_007408259.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NPS25_RS12995 (NPS25_12995) - 2669914..2670483 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  NPS25_RS13000 (NPS25_13000) yhbY 2670494..2670784 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86778.50 Da        Isoelectric Point: 8.6684

>NTDB_id=712590 NPS25_RS12960 WP_256683591.1 2663705..2666056(-) (comEC) [Bacillus velezensis strain Ba-0321]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKQYAPVIVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIEQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGESRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFGMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTFFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSISEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVFDGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=712590 NPS25_RS12960 WP_256683591.1 2663705..2666056(-) (comEC) [Bacillus velezensis strain Ba-0321]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCAGTATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGCTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATTACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAGTG
GGCGGCTTCGTACAAAATCCGTTCTCTTGAGGAAAAAAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTGAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCTTGTCTTATCTGCTGCTTCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGGAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCTTTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTCTCAAGGCAGATGGGAGAATGTTTGTTTGGTATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGTTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCAGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCATATTCTATCAGCGAGAAGGTTTTGATTCCATTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTAGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGGGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTTGACGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCACTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(25-171)

Competence protein (Competence)

(208-468)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

56.606

98.595

0.558