Detailed information NTG0000107

Experimentally validated

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: BSU_25570
• Genome accession: NC_000964
• Coordinates: 2637543..2639873 (-)
• Length: 776 a.a.
• NCBI ID: NP_390435.1
• Uniprot ID: P39695
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: ssDNA transport into the cell DNA binding and uptake

Function

ComEC is required for the internalization of single-stranded DNA into the cytoplasm. It forms a transmembrane channel that facilitates the transport of one strand of the incoming double-stranded DNA, while the other strand is degraded. Recent studies have shown that the C-terminal domain (CTD) of ComEC has metal-dependent nuclease activity, which is important for degrading the non-transforming DNA strand during natural transformation. This nuclease activity is crucial for efficient DNA import and ensures that only one strand of the DNA is transported into the cell.

Genomic Context

Location: 2632543..2644873

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_25520 (BSU25520)	yqxA	2632882..2633220 (-)	339	NP_390430.1	hypothetical protein	-
BSU_25530 (BSU25530)	spoIIP	2633237..2634442 (-)	1206	NP_390431.1	spore autolysin (stage II sporulation)	-
BSU_25540 (BSU25540)	gpr	2634505..2635611 (-)	1107	NP_390432.1	spore germination protease	-
BSU_25550 (BSU25550)	rpsT	2635815..2636081 (+)	267	NP_390433.2	ribosomal protein S20 (BS20)	-
BSU_25560 (BSU25560)	holA	2636096..2637139 (-)	1044	NP_390434.1	DNA polymerase clamp loader delta subunit	-
BSU_25565 (BSU25565)	-	2637179..2637328 (-)	150	YP_009513978.1	hypothetical protein	-
BSU_25569 (BSU25569)	yqzM	2637369..2637503 (+)	135	YP_003097762.1	hypothetical protein	-
BSU_25570 (BSU25570)	comEC	2637543..2639873 (-)	2331	NP_390435.1	DNA channel for uptake in competent cells	Machinery gene
BSU_25580 (BSU25580)	comEB	2639877..2640446 (-)	570	NP_390436.1	dCMP deaminase associated to DNA transport (competence)	Regulator
BSU_25590 (BSU25590)	comEA	2640513..2641130 (-)	618	NP_390437.1	membrane bound high-affinity DNA-binding receptor	Machinery gene
BSU_25600 (BSU25600)	comER	2641214..2642035 (+)	822	NP_390438.1	putative pyrroline-5'-carboxylate reductase	-
BSU_25610 (BSU25610)	yqeM	2642101..2642844 (-)	744	NP_390439.1	putative methyltransferase	-
BSU_25620 (BSU25620)	rsfS	2642841..2643197 (-)	357	NP_390440.1	ribosomal silencing factor	-
BSU_25630 (BSU25630)	yqeK	2643215..2643775 (-)	561	NP_390441.1	putative hydrolase	-
BSU_25640 (BSU25640)	nadD	2643765..2644334 (-)	570	NP_390442.1	nicotinate-nucleotide adenylyltransferase	-
BSU_25650 (BSU25650)	yqeI	2644346..2644636 (-)	291	NP_390443.1	50S RNA-binding protein	-

Sequence

Protein

• Length: 776 a.a.
• Molecular weight: 86701.36 Da
• Isoelectric Point: 7.3497

>NTDB_id=107 BSU_25570 NP_390435.1 2637543..2639873(-) (comEC) [Bacillus subtilis subsp. subtilis str. 168]
MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIILIKTRHAFLIIVCFFSFILFFVLYAVTDSQNVSSYRQGTYQF
KAVIDTIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKEQLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHI
HWNYSVTSIQNCSEPENFKYKVLSLRKHIISFTNSLLPPDSTGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHV
GILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKWRVRSATAICLSYIVLLLFNP
YHLFEAGFQLSFAVSFSLILSSSIFQQVKTSLGQLTIVSLIAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGA
VAGVLLLSLSASFGRLFFSWFDLLISWINRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLMVTGG
ICCTVMFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWREKQHPFSLGEKVLIPFLTAKG
IKQLDALILTHADQDHIGEAEILLKHHKVKRLVIPKGFVSEPKDEKVLQAAREEGVAIEEVKRGDVLQIKDLQFHVLSPE
APDPASKNNSSLVLWMETGGMSWILTGDLEKEGEQEVMNVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKN
NRYHHPHQKVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN

Nucleotide

• Length: 2331 bp

>NTDB_id=107 BSU_25570 NP_390435.1 2637543..2639873(-) (comEC) [Bacillus subtilis subsp. subtilis str. 168]
ATGCGTAATTCGCGCTTATTATTGCCTATGGCGGCAGCTTCGGCAACGGCTGGAATTACTGCCGCCGCTTATTTCCCCGC
TATTTTTCTTTTCATCCTCTTTCTCCTCATCATTTTAATCAAAACGAGGCACGCTTTTCTTATTATTGTTTGTTTCTTCT
CTTTTATATTGTTTTTTGTACTGTATGCAGTCACAGATTCTCAGAATGTCTCTTCCTATCGGCAGGGAACCTATCAATTC
AAGGCAGTGATTGACACTATTCCCAAAATTGACGGCGACCGTATGTCTATGATGGTTGAGACACCTGATAAGGAAAAATG
GGCTGCTGCGTATCGCATTCAGTCTGCTGGTGAAAAAGAACAGCTGTTATACATAGAACCAGGAATGTCATGTGAGTTGA
CTGGTACATTGGAAGAACCGAATCACGCAACTGTGCCGGGTGCATTTGATTATAACGAGTATCTTTATCGGCAGCATATT
CATTGGAACTACTCTGTCACGTCTATCCAAAACTGCAGCGAACCTGAAAATTTCAAGTACAAGGTGCTCAGCTTGAGAAA
ACATATCATATCATTCACAAACAGCCTTCTGCCTCCTGATTCGACAGGGATTGTACAGGCACTTACAGTTGGTGACAGAT
TTTACGTGGAGGATGAAGTGCTTACCGCGTATCAAAAGCTTGGTGTTGTCCATCTCTTGGCAATATCAGGACTCCACGTG
GGGATTTTGACAGCAGGTTTGTTTTATATCATGATCCGCCTTGGTATAACTAGAGAAAAGGCGTCAATTCTGTTGCTGTT
ATTTCTGCCGCTCTATGTGATGTTGACCGGCGCTGCTCCTTCAGTGCTACGCGCCGCTCTCATGTCGGGTGTTTACTTAG
CTGGAAGTCTTGTCAAATGGCGTGTCCGCTCTGCAACTGCAATTTGCCTTTCGTACATCGTCCTTCTGCTCTTCAATCCT
TATCATCTCTTTGAAGCCGGTTTTCAGCTATCGTTCGCCGTCAGTTTTTCTTTAATTCTATCCTCTTCTATTTTTCAGCA
GGTTAAAACCTCCTTGGGGCAGCTGACAATTGTATCACTCATCGCTCAGCTGGGCTCGCTTCCGATTCTCCTATATCATT
TTCATCAGTTTTCTATCATCAGCGTACCGATGAATATGTTGATGGTACCATTTTATACCTTCTGTATTTTGCCGGGAGCT
GTAGCAGGTGTTCTTCTATTAAGTCTTTCCGCTTCGTTTGGGAGATTGTTTTTCAGCTGGTTTGATTTATTGATAAGCTG
GATCAATAGGTTAATCACAAACATTGCAGATGTTGATGTGTTCACGATTATGATCGCACATCCTGCACCTGTTTTGCTTT
TTTTATTCACAGTCACGATCATCCTATTGCTTATGGCGATTGAAAAACGCTCCTTGTCGCAGTTGATGGTAACCGGAGGC
ATTTGCTGCACGGTGATGTTTCTGCTCTTTATATATCCGTGTCTTAGTTCCGAAGGAGAAGTGGATATGATAGATATTGG
ACAGGGTGACAGCATGTTTGTAGGTGCTCCGCATCAGCGGGGTCGTGTCTTAATTGATACCGGCGGCACTTTGTCTTACT
CGTCAGAGCCTTGGCGCGAAAAACAGCATCCGTTTTCACTGGGGGAAAAGGTGCTGATTCCGTTTTTAACTGCTAAGGGA
ATCAAGCAGCTTGACGCTTTAATTCTGACGCACGCTGACCAAGATCATATCGGAGAGGCGGAGATTCTGCTGAAGCATCA
TAAAGTAAAGCGCCTCGTGATTCCGAAAGGGTTCGTTTCTGAACCTAAAGATGAGAAAGTGCTGCAGGCAGCCAGGGAAG
AGGGAGTGGCAATTGAAGAGGTGAAGCGAGGCGATGTGTTGCAAATAAAGGATTTGCAGTTCCATGTACTGTCACCGGAA
GCACCTGATCCGGCAAGCAAAAATAATTCATCTCTCGTTCTGTGGATGGAGACGGGCGGTATGAGCTGGATCTTGACGGG
TGATCTGGAGAAAGAAGGGGAACAAGAGGTGATGAACGTGTTTCCGAATATAAAAGCAGATGTGTTAAAGGTGGGGCACC
ATGGGAGCAAAGGCTCTACCGGTGAAGAATTCATCCAACAGCTTCAGCCGAAAACGGCCATTATCTCAGCCGGGAAAAAC
AATCGGTACCATCATCCTCATCAAAAAGTTCTGCAACTATTACAGAGACATTCTATCCGCGTGCTGCGAACAGATCAAAA
CGGAACGATCCAATATAGATACAAAAACAGAGTTGGAACCTTTTCTGTCTATCCTCCATATGATACATCAGATATAACAG
AGACGAACTAA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (20-170)

• Metallo-beta-lactamase superfamily (Lactamase_B) (507-718)

3D structure

• AlphaFold DB: P39695

References

• [1]: Marie Burghard-Schrod et al. (2022) Single-Molecule Dynamics of DNA Receptor ComEA, Membrane Permease ComEC, and Taken-Up DNA in Competent Bacillus subtilis Cells. Journal of Bacteriology 204(3):e0057221. [PMID: 34928178]
• [2]: Micaela De Santis et al. (2021) ComEB protein is dispensable for the transformation but must be translated for the optimal synthesis of comEC. Molecular Microbiology 116(1):71-79. [PMID: 33527432]
• [3]: Augustinas Silale et al. (2021) The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation. Molecular Microbiology 116(2):416-426. [PMID: 33772889]
• [4]: James A Baker et al. (2016) Potential DNA binding and nuclease functions of ComEC domains characterized in silico. Proteins 84(10):1431-42. [PMID: 27318187]
• [5]: Masaomi Takeno et al. (2011) Role of ComFA in controlling the DNA uptake rate during transformation of competent Bacillus subtilis. Journal of Bioscience And Bioengineering 111(6):618-23. [PMID: 21397556]
• [6]: Naomi Kramer et al. (2007) Multiple interactions among the competence proteins of Bacillus subtilis. Molecular Microbiology 65(2):454-64. [PMID: 17630974]
• [7]: Inês Chen et al. (2006) A macromolecular complex formed by a pilin-like protein in competent Bacillus subtilis. The Journal of Biological Chemistry 281(31):21720-21727. [PMID: 16751195]
• [8]: Irena Draskovic et al. (2005) Biogenesis of a putative channel protein, ComEC, required for DNA uptake: membrane topology, oligomerization and formation of disulphide bonds. Molecular Microbiology 55(3):881-96. [PMID: 15661011]
• [9]: G S Inamine et al. (1995) ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport. Journal of Bacteriology 177(11):3045-51. [PMID: 7768800]