Detailed information NTG0000106

Experimentally validated

Overview

• Name: comEB
• Type: Regulator
• Locus tag: BSU_25580
• Genome accession: NC_000964
• Coordinates: 2639877..2640446 (-)
• Length: 189 a.a.
• NCBI ID: NP_390436.1
• Uniprot ID: P32393
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: synthesis of competence genes Competence regulation

Function

ComEB is not required for the actual process of transformation but is required for the optimal synthesis of ComEC, a membrane permease involved in DNA uptake. This translational coupling ensures the correct stoichiometry of proteins within the competence machinery.

Genomic Context

Location: 2634877..2645446

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_25550 (BSU25550)	rpsT	2635815..2636081 (+)	267	NP_390433.2	ribosomal protein S20 (BS20)	-
BSU_25560 (BSU25560)	holA	2636096..2637139 (-)	1044	NP_390434.1	DNA polymerase clamp loader delta subunit	-
BSU_25565 (BSU25565)	-	2637179..2637328 (-)	150	YP_009513978.1	hypothetical protein	-
BSU_25569 (BSU25569)	yqzM	2637369..2637503 (+)	135	YP_003097762.1	hypothetical protein	-
BSU_25570 (BSU25570)	comEC	2637543..2639873 (-)	2331	NP_390435.1	DNA channel for uptake in competent cells	Machinery gene
BSU_25580 (BSU25580)	comEB	2639877..2640446 (-)	570	NP_390436.1	dCMP deaminase associated to DNA transport (competence)	Regulator
BSU_25590 (BSU25590)	comEA	2640513..2641130 (-)	618	NP_390437.1	membrane bound high-affinity DNA-binding receptor	Machinery gene
BSU_25600 (BSU25600)	comER	2641214..2642035 (+)	822	NP_390438.1	putative pyrroline-5'-carboxylate reductase	-
BSU_25610 (BSU25610)	yqeM	2642101..2642844 (-)	744	NP_390439.1	putative methyltransferase	-
BSU_25620 (BSU25620)	rsfS	2642841..2643197 (-)	357	NP_390440.1	ribosomal silencing factor	-
BSU_25630 (BSU25630)	yqeK	2643215..2643775 (-)	561	NP_390441.1	putative hydrolase	-
BSU_25640 (BSU25640)	nadD	2643765..2644334 (-)	570	NP_390442.1	nicotinate-nucleotide adenylyltransferase	-
BSU_25650 (BSU25650)	yqeI	2644346..2644636 (-)	291	NP_390443.1	50S RNA-binding protein	-

Sequence

Protein

• Length: 189 a.a.
• Molecular weight: 20969.05 Da
• Isoelectric Point: 5.5485

>NTDB_id=106 BSU_25580 NP_390436.1 2639877..2640446(-) (comEB) [Bacillus subtilis subsp. subtilis str. 168]
MERISWNQYFMAQSHLLALRSTCPRLSVGATIVRDKRMIAGGYNGSIAGGVHCADEGCLMIDDHCARTIHAEMNAILQCS
KFGVPTDGAEIYVTHYPCIQCCKSIIQAGIKTVYYAEDYKTHPYAQELFEQAGVTVEQVELDEMIVDLKNREKLSFVAGL
IGKLADAGLAEEELKKIHEQANTLFTSYV

Nucleotide

• Length: 570 bp

>NTDB_id=106 BSU_25580 NP_390436.1 2639877..2640446(-) (comEB) [Bacillus subtilis subsp. subtilis str. 168]
GTGGAACGAATTTCATGGAATCAATACTTTATGGCTCAAAGCCATTTATTGGCGCTGCGCAGCACATGTCCGAGATTATC
TGTCGGCGCTACTATAGTCAGAGACAAACGCATGATAGCAGGAGGCTACAATGGGTCAATCGCGGGCGGCGTACATTGTG
CAGATGAAGGATGCTTAATGATAGATGATCATTGCGCAAGAACCATTCACGCTGAAATGAATGCGATTCTTCAATGCTCT
AAGTTTGGGGTGCCGACTGATGGAGCCGAGATTTATGTGACGCATTATCCGTGCATTCAATGCTGCAAATCTATTATTCA
AGCTGGTATCAAAACGGTTTATTATGCTGAGGATTACAAAACACATCCGTATGCGCAGGAATTATTTGAACAGGCGGGTG
TAACTGTTGAACAGGTTGAACTCGATGAAATGATCGTTGATCTGAAAAACAGAGAAAAGCTTTCATTTGTAGCGGGTCTA
ATAGGGAAATTGGCTGATGCCGGTTTAGCGGAGGAAGAATTGAAAAAGATACATGAACAGGCGAATACACTTTTCACGAG
CTACGTGTGA

Domains

Predicted by InterproScan.

• Cytidine and deoxycytidylate deaminase zinc-binding region (dCMP_cyt_deam_1) (5-116)

3D structure

• AlphaFold DB: P32393

References

• [1]: Micaela De Santis et al. (2021) ComEB protein is dispensable for the transformation but must be translated for the optimal synthesis of comEC. Molecular Microbiology 116(1):71-79. [PMID: 33527432]
• [2]: Marie Burghard-Schrod et al. (2020) The Bacillus subtilis dCMP deaminase ComEB acts as a dynamic polar localization factor for ComGA within the competence machinery. Molecular Microbiology 113(5):906-922. [PMID: 31954084]
• [3]: G S Inamine et al. (1995) ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport. Journal of Bacteriology 177(11):3045-51. [PMID: 7768800]