Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NM620_RS01030 Genome accession   NZ_CP101694
Coordinates   220462..222183 (+) Length   573 a.a.
NCBI ID   WP_080174369.1    Uniprot ID   A0A1T4SAF2
Organism   Photobacterium toruni strain WD2103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 215462..227183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM620_RS01010 pilM 217756..218736 (+) 981 WP_256383538.1 type IV pilus biogenesis protein PilM -
  NM620_RS01015 - 218733..219344 (+) 612 WP_080174366.1 PilN domain-containing protein -
  NM620_RS01020 - 219337..219921 (+) 585 WP_080174367.1 type 4a pilus biogenesis protein PilO -
  NM620_RS01025 - 219933..220448 (+) 516 WP_256383539.1 pilus assembly protein PilP -
  NM620_RS01030 pilQ 220462..222183 (+) 1722 WP_080174369.1 type IV pilus secretin PilQ Machinery gene
  NM620_RS01035 aroK 222571..223089 (+) 519 WP_045035958.1 shikimate kinase AroK -
  NM620_RS01040 aroB 223113..224192 (+) 1080 WP_256383540.1 3-dehydroquinate synthase -
  NM620_RS01045 - 224218..225483 (+) 1266 WP_256383541.1 AAA family ATPase -
  NM620_RS01050 dam 225592..226425 (+) 834 WP_080174372.1 adenine-specific DNA-methyltransferase -
  NM620_RS01055 - 226437..226637 (-) 201 WP_256383542.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62489.54 Da        Isoelectric Point: 5.8393

>NTDB_id=711564 NM620_RS01030 WP_080174369.1 220462..222183(+) (pilQ) [Photobacterium toruni strain WD2103]
MKGQTWGLSRQIFVSVWLICWGLLTVTSAAATPASGITNQVQDIKFHRDGKDRGIFTVSLKQPQSLVDIRRQGKKLQLDI
FDTQLTNEQIHTLDVADFATVVNSIDTSNVPRGARFVFNMTGRYDYDYRIRGNNLEVTLIKRTPPKATSTTHKSKVSYKG
KPISINFQDIPVRNVLQLIADYNDFNLVVADTVSGNVTLRLDNVPWPQVLDIILQAKGLDKRVRGSVLLVAPKAELAANE
QQVMETARKQQELASLRSELIQINYAKATDISELLSGSSDGVGMLSERGSLHVDDRTNALIIKDTPENIASIKDIVLALD
IPIKQVQIEARIVTIDEGNIDELGVRWGVSSIDGNTTIGGSIEGNLGSSGLLDDTTVDDFLNVNLGATQAGAASIAFQVA
KLGNVLLDLELSALQSERKAEIISSPRLMTTNKKTAYIEQGTEIPYLEASSSGAVSVDFKKAVLSLMVTPQITPDNKLVL
DLEVTQDSKGEEVPTGVGTAVAIKTQRIGTQVLVNNGETVVLGGIYQNQVNNTVRKVPILGDIPVLGALFRHKMEELGKR
ELLIFVTPKIVMQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=711564 NM620_RS01030 WP_080174369.1 220462..222183(+) (pilQ) [Photobacterium toruni strain WD2103]
ATGAAAGGGCAGACATGGGGGCTTAGCCGCCAGATTTTCGTCAGTGTATGGTTAATATGCTGGGGATTACTGACGGTAAC
GAGCGCAGCAGCTACGCCAGCAAGTGGTATTACTAATCAAGTTCAAGATATTAAATTCCATCGTGATGGTAAAGATCGAG
GCATATTTACTGTTAGTTTGAAACAGCCGCAATCATTAGTTGATATTCGCCGCCAAGGTAAAAAATTACAGTTGGATATT
TTTGATACTCAATTAACCAACGAACAAATACATACTTTAGATGTGGCTGATTTTGCGACAGTTGTAAATAGCATTGATAC
CAGCAACGTGCCTCGTGGAGCACGGTTTGTATTTAATATGACGGGTCGTTATGACTACGATTACCGTATTCGAGGTAATA
ATCTGGAAGTGACTTTAATTAAACGAACGCCCCCTAAAGCGACATCAACGACACACAAAAGTAAGGTCTCTTACAAGGGC
AAGCCAATTTCAATTAACTTTCAAGATATTCCCGTGCGTAATGTTTTACAGCTTATTGCCGATTACAATGATTTTAACTT
GGTCGTTGCAGACACTGTCAGTGGTAACGTCACGCTTCGTTTAGATAATGTGCCATGGCCGCAAGTGCTGGATATTATTC
TGCAAGCAAAAGGGTTAGATAAGCGAGTGCGTGGTTCAGTACTCTTGGTTGCCCCTAAGGCTGAACTCGCGGCTAATGAA
CAACAAGTGATGGAAACCGCGCGGAAACAGCAAGAATTAGCGAGTCTACGCTCTGAACTGATTCAAATAAATTACGCAAA
AGCGACAGATATTTCAGAGTTGTTAAGTGGCTCAAGTGATGGTGTTGGAATGTTATCTGAACGGGGGTCGTTACATGTTG
ATGATCGAACTAATGCATTGATAATTAAAGATACACCAGAAAATATTGCTAGTATTAAGGATATCGTGTTAGCGCTTGAT
ATTCCGATTAAGCAAGTACAAATAGAAGCTCGAATTGTTACTATTGATGAAGGTAATATCGATGAATTAGGGGTTCGTTG
GGGGGTATCTAGCATTGATGGTAACACAACGATCGGTGGCTCGATTGAAGGTAACCTTGGTAGTTCTGGATTATTAGATG
ACACCACTGTTGATGATTTTCTTAACGTTAACTTAGGGGCAACTCAAGCGGGAGCTGCAAGTATTGCTTTTCAAGTAGCA
AAGTTAGGTAATGTGTTACTTGATTTAGAATTATCAGCGTTACAGTCAGAGCGTAAAGCTGAAATTATCTCAAGCCCACG
CTTAATGACGACCAATAAAAAAACCGCTTATATTGAGCAAGGCACAGAAATACCGTATCTAGAGGCATCATCAAGTGGTG
CAGTATCGGTTGATTTTAAGAAAGCAGTATTAAGCCTTATGGTAACACCTCAAATTACCCCCGATAATAAGCTGGTACTT
GATCTAGAAGTCACTCAAGACAGTAAAGGGGAAGAGGTGCCAACAGGGGTTGGTACAGCGGTAGCGATTAAAACGCAACG
TATCGGTACGCAAGTGTTAGTGAATAATGGAGAAACAGTCGTATTAGGTGGTATTTATCAAAACCAAGTTAATAATACCG
TTCGTAAAGTGCCTATTCTTGGTGATATTCCAGTGCTTGGCGCATTATTTAGACATAAAATGGAAGAACTTGGAAAACGT
GAATTATTGATTTTTGTGACGCCAAAAATTGTGATGCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(413-571)

Bacterial type II/III secretion system short domain (Secretin_N)

(259-325)

Secretin and TonB N terminus short domain (STN)

(185-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T4SAF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

59.115

98.604

0.583
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

59.115

98.604

0.583
  pilQ Vibrio campbellii strain DS40M4

60.036

95.637

0.574
  pilQ Pseudomonas aeruginosa PAK

43.739

96.161

0.421
  comQ Acinetobacter baylyi ADP1

40.728

100

0.41
  pilQ Acinetobacter baumannii D1279779

39.861

100

0.401
  pilQ Acinetobacter baumannii strain A118

39.861

100

0.401
  pilQ Legionella pneumophila strain Lp02

43.359

89.354

0.387
  pilQ Legionella pneumophila strain ERS1305867

43.359

89.354

0.387