Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NON14_RS04780 Genome accession   NZ_CP101642
Coordinates   970088..970978 (+) Length   296 a.a.
NCBI ID   WP_409043703.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain HRB10     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 965088..975978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NON14_RS04745 (NON14_04745) - 965312..966325 (+) 1014 WP_025016377.1 YeiH family protein -
  NON14_RS04750 (NON14_04750) - 966391..966630 (-) 240 WP_011375006.1 cytochrome b5 domain-containing protein -
  NON14_RS04755 (NON14_04755) - 966870..967949 (+) 1080 WP_112217756.1 zinc ribbon domain-containing protein -
  NON14_RS04765 (NON14_04765) - 968179..968553 (-) 375 WP_099947400.1 hypothetical protein -
  NON14_RS04770 (NON14_04770) - 968650..969150 (+) 501 WP_076632058.1 VanZ family protein -
  NON14_RS04775 (NON14_04775) - 969241..969972 (+) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  NON14_RS04780 (NON14_04780) comGA 970088..970978 (+) 891 WP_409043703.1 competence type IV pilus ATPase ComGA Machinery gene
  NON14_RS04785 (NON14_04785) comGB 970971..971978 (+) 1008 WP_056947133.1 type II secretion system F family protein Machinery gene
  NON14_RS04790 (NON14_04790) comGC 971975..972274 (+) 300 WP_409043704.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NON14_RS04795 (NON14_04795) comGD 972252..972698 (+) 447 WP_409043713.1 competence type IV pilus minor pilin ComGD Machinery gene
  NON14_RS04800 (NON14_04800) comGE 972685..972990 (+) 306 WP_025016385.1 hypothetical protein Machinery gene
  NON14_RS04805 (NON14_04805) comGF 972965..973477 (+) 513 WP_025016386.1 competence type IV pilus minor pilin ComGF Machinery gene
  NON14_RS04810 (NON14_04810) - 973440..973778 (+) 339 WP_056947123.1 hypothetical protein -
  NON14_RS04815 (NON14_04815) - 973893..974912 (+) 1020 WP_241652828.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32930.79 Da        Isoelectric Point: 6.6039

>NTDB_id=711132 NON14_RS04780 WP_409043703.1 970088..970978(+) (comGA) [Latilactobacillus sakei strain HRB10]
MSTEEKLTTLLKHCAKQRVQDLYFTPTIGGWHLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSKQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGPDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=711132 NON14_RS04780 WP_409043703.1 970088..970978(+) (comGA) [Latilactobacillus sakei strain HRB10]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAACAACGTGTTCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCACTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCACAAACCGGCCGCTCGGAATTA
CAGTTAGCCGACCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAATCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGCAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCCATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTTAATGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAAATTCGCGATTTAG
AGACAGCTCAATATGCAGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTAAACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCAGAGCTGCAATTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTCCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

98.311

100

0.983
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.642

89.527

0.382
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

40.876

92.568

0.378
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae R6

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae D39

42.264

89.527

0.378
  comGA Lactococcus lactis subsp. cremoris KW2

37.458

100

0.378
  comYA Streptococcus gordonii str. Challis substr. CH1

40.59

91.554

0.372