Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NON14_RS04805 Genome accession   NZ_CP101642
Coordinates   972965..973477 (+) Length   170 a.a.
NCBI ID   WP_025016386.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain HRB10     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 967965..978477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NON14_RS04765 (NON14_04765) - 968179..968553 (-) 375 WP_099947400.1 hypothetical protein -
  NON14_RS04770 (NON14_04770) - 968650..969150 (+) 501 WP_076632058.1 VanZ family protein -
  NON14_RS04775 (NON14_04775) - 969241..969972 (+) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  NON14_RS04780 (NON14_04780) comGA 970088..970978 (+) 891 WP_409043703.1 competence type IV pilus ATPase ComGA Machinery gene
  NON14_RS04785 (NON14_04785) comGB 970971..971978 (+) 1008 WP_056947133.1 type II secretion system F family protein Machinery gene
  NON14_RS04790 (NON14_04790) comGC 971975..972274 (+) 300 WP_409043704.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NON14_RS04795 (NON14_04795) comGD 972252..972698 (+) 447 WP_409043713.1 competence type IV pilus minor pilin ComGD Machinery gene
  NON14_RS04800 (NON14_04800) comGE 972685..972990 (+) 306 WP_025016385.1 hypothetical protein Machinery gene
  NON14_RS04805 (NON14_04805) comGF 972965..973477 (+) 513 WP_025016386.1 competence type IV pilus minor pilin ComGF Machinery gene
  NON14_RS04810 (NON14_04810) - 973440..973778 (+) 339 WP_056947123.1 hypothetical protein -
  NON14_RS04815 (NON14_04815) - 973893..974912 (+) 1020 WP_241652828.1 class I SAM-dependent methyltransferase -
  NON14_RS04820 (NON14_04820) - 974934..976130 (+) 1197 WP_011374993.1 acetate/propionate family kinase -
  NON14_RS04825 (NON14_04825) - 976185..976643 (+) 459 WP_112206089.1 laaL -
  NON14_RS04835 (NON14_04835) - 977185..977550 (-) 366 WP_011374991.1 DUF805 domain-containing protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19690.57 Da        Isoelectric Point: 6.6472

>NTDB_id=711137 NON14_RS04805 WP_025016386.1 972965..973477(+) (comGF) [Latilactobacillus sakei strain HRB10]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLDGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=711137 NON14_RS04805 WP_025016386.1 972965..973477(+) (comGF) [Latilactobacillus sakei strain HRB10]
TTGCGATTAATTGGACGACCGATTAAACACTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTGACACAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCAGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTCGTTGTCAGCCAATTAGATGGGTATATGCCATTATTAGGTAACCTCAAGCAACTTCAACTTGACTACCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATTAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.824

100

0.988