Detailed information NTG0710839

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NNG64_RS05260
• Genome accession: NZ_CP101610
• Coordinates: 1001388..1002002 (+)
• Length: 204 a.a.
• NCBI ID: WP_060964714.1
• Uniprot ID: A0AAI8HMV7
• Organism: Bacillus siamensis strain WYJ-E14
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 996388..1007002

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NNG64_RS05225 (NNG64_05215)	aroE	997076..997918 (+)	843	WP_060964710.1	shikimate dehydrogenase	-
NNG64_RS05230 (NNG64_05220)	yhbY	997912..998202 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
NNG64_RS05235 (NNG64_05225)	-	998213..998782 (+)	570	WP_016938889.1	nicotinate-nucleotide adenylyltransferase	-
NNG64_RS05240 (NNG64_05230)	yqeK	998772..999332 (+)	561	WP_060964711.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NNG64_RS05245 (NNG64_05235)	rsfS	999349..999705 (+)	357	WP_016938891.1	ribosome silencing factor	-
NNG64_RS05250 (NNG64_05240)	-	999702..1000439 (+)	738	WP_060964712.1	class I SAM-dependent methyltransferase	-
NNG64_RS05255 (NNG64_05245)	comER	1000508..1001329 (-)	822	WP_060964713.1	late competence protein ComER	-
NNG64_RS05260 (NNG64_05250)	comEA	1001388..1002002 (+)	615	WP_060964714.1	helix-hairpin-helix domain-containing protein	Machinery gene
NNG64_RS05265 (NNG64_05255)	-	1002068..1002637 (+)	570	WP_016938895.1	ComE operon protein 2	-
NNG64_RS05270 (NNG64_05260)	comEC	1002638..1004989 (+)	2352	WP_060964715.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NNG64_RS05275 (NNG64_05265)	-	1005008..1005142 (-)	135	WP_003152870.1	YqzM family protein	-
NNG64_RS05280 (NNG64_05270)	-	1005183..1005337 (+)	155	Protein_998	hypothetical protein	-
NNG64_RS05285 (NNG64_05275)	holA	1005377..1006418 (+)	1042	Protein_999	DNA polymerase III subunit delta	-
NNG64_RS05290 (NNG64_05280)	rpsT	1006436..1006702 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22272.36 Da
• Isoelectric Point: 4.7944

>NTDB_id=710839 NNG64_RS05260 WP_060964714.1 1001388..1002002(+) (comEA) [Bacillus siamensis strain WYJ-E14]
MTKAMMTEQMRRNLKILGVCLAAGAVFICIWLFRPEKEEPVKQQSVLGQTASASGTDKTDTADDKIMIDMKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKEKADELNVNLAEQLQDGTVVYIPSEGEEENRPKTAAGEKEDAPVNINTATLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=710839 NNG64_RS05260 WP_060964714.1 1001388..1002002(+) (comEA) [Bacillus siamensis strain WYJ-E14]
ATGACGAAAGCGATGATGACGGAGCAGATGCGGCGGAACCTGAAAATTCTGGGTGTTTGTCTTGCGGCAGGAGCGGTTTT
TATCTGCATTTGGCTGTTTCGTCCTGAAAAAGAAGAACCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGGCACGGACAAAACAGACACCGCAGACGATAAGATTATGATTGATATGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAGGCGGGAGACAGAGTGACACAAGCCATTGAAAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAAGACGGGACGGTCGTTTATATTCCGAGCGAAGGCGAAGAGGAGAATCGCCCGAAAACCG
CAGCAGGTGAGAAGGAGGATGCGCCTGTCAATATCAATACGGCAACTCTGGACGAACTTCAGGCGATTTCCGGTGTGGGG
CAGAAAAAAGCGGAAGCAATTATCGCCTACCGGGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (142-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.5	98.039	0.574
comEA	Lactococcus lactis subsp. cremoris KW2	36.715	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.232	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis SK321	35.749	100	0.363