Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   NNG64_RS05270 Genome accession   NZ_CP101610
Coordinates   1002638..1004989 (+) Length   783 a.a.
NCBI ID   WP_060964715.1    Uniprot ID   A0AAI8HMV4
Organism   Bacillus siamensis strain WYJ-E14     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 997638..1009989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNG64_RS05230 (NNG64_05220) yhbY 997912..998202 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  NNG64_RS05235 (NNG64_05225) - 998213..998782 (+) 570 WP_016938889.1 nicotinate-nucleotide adenylyltransferase -
  NNG64_RS05240 (NNG64_05230) yqeK 998772..999332 (+) 561 WP_060964711.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NNG64_RS05245 (NNG64_05235) rsfS 999349..999705 (+) 357 WP_016938891.1 ribosome silencing factor -
  NNG64_RS05250 (NNG64_05240) - 999702..1000439 (+) 738 WP_060964712.1 class I SAM-dependent methyltransferase -
  NNG64_RS05255 (NNG64_05245) comER 1000508..1001329 (-) 822 WP_060964713.1 late competence protein ComER -
  NNG64_RS05260 (NNG64_05250) comEA 1001388..1002002 (+) 615 WP_060964714.1 helix-hairpin-helix domain-containing protein Machinery gene
  NNG64_RS05265 (NNG64_05255) - 1002068..1002637 (+) 570 WP_016938895.1 ComE operon protein 2 -
  NNG64_RS05270 (NNG64_05260) comEC 1002638..1004989 (+) 2352 WP_060964715.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NNG64_RS05275 (NNG64_05265) - 1005008..1005142 (-) 135 WP_003152870.1 YqzM family protein -
  NNG64_RS05280 (NNG64_05270) - 1005183..1005337 (+) 155 Protein_998 hypothetical protein -
  NNG64_RS05285 (NNG64_05275) holA 1005377..1006418 (+) 1042 Protein_999 DNA polymerase III subunit delta -
  NNG64_RS05290 (NNG64_05280) rpsT 1006436..1006702 (-) 267 WP_003152876.1 30S ribosomal protein S20 -
  NNG64_RS05295 (NNG64_05285) gpr 1006905..1008011 (+) 1107 WP_060964717.1 GPR endopeptidase -
  NNG64_RS05300 (NNG64_05290) spoIIP 1008079..1009272 (+) 1194 WP_060964718.1 stage II sporulation protein P -
  NNG64_RS05305 (NNG64_05295) - 1009289..1009627 (+) 339 WP_016938900.1 YqxA family protein -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 87150.01 Da        Isoelectric Point: 9.1532

>NTDB_id=710841 NNG64_RS05270 WP_060964715.1 1002638..1004989(+) (comEC) [Bacillus siamensis strain WYJ-E14]
MKYKYLLLPLAAVSATAGIAAANFFWVLFFFLLYLLFLFVKTKQYTPFIVSLVSFCLYFFLYTVCDAANVSRYQGGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKSLIEQLEPGMRCVFTGSLEQPGHATIPGGFDYKEHLNSQHI
HWLFSVTSIQQCEKSGMPLYKLLNIRKNLISIIQNHVPESSAGIVEALTLGERFSIEDDILNAYQNLGVVHLMAISGMHV
GLITAGIFYVLIRIGLTREKAGVLLLFFLPVYTLLSGAAPSVLRASLMLGFYIAGTLFKRRIHSSAALSVSYLLLLMFNP
YLLWQAGFQLSFAVSAALILSSSILKKAGKRRFAALAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPF
SVIGFLLLLLSRQVGECLFYMFDLVMKPVHDFMTYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSP
LFFCAVLVYLMFRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVVAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKKHRVKRLIVPVGFVKEPKDQDILKMAKENNIPVAEAKRGDTITAGHLQFQVLSP
ESSDGRSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKAYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGE
DNRYHHPHEEVLDRLKAYSVNVLRTDLSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=710841 NNG64_RS05270 WP_060964715.1 1002638..1004989(+) (comEC) [Bacillus siamensis strain WYJ-E14]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACCGCGGGAATTGCCGCCGCAAATTTCTTCTGGGT
TCTATTCTTTTTTCTTCTGTATCTTCTCTTTCTTTTTGTAAAAACAAAGCAGTACACACCGTTTATCGTCAGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTGTATACGGTTTGTGACGCTGCGAATGTATCCCGGTATCAGGGCGGGAGCTATACTGAA
CAGGCCGTCATCACAAATATTCCGAAGGTTGACGGGGCGAAAATGTCTGCCGTTATCCGAACGCATGACAAAGAAAAATG
GGCCGCTTCATACAAAATCCGGTCTCTTGAAGAAAAAAGTCTCATTGAACAGCTGGAACCGGGGATGCGCTGCGTGTTTA
CCGGCTCTTTGGAACAGCCCGGACATGCGACGATTCCCGGAGGTTTTGATTATAAGGAACATCTTAATTCTCAGCATATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCGGCATGCCGCTGTATAAATTGCTCAACATCAGAAA
GAATTTGATTTCGATCATTCAAAATCACGTGCCTGAATCCTCTGCCGGAATTGTAGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAACGCTTATCAAAATCTGGGAGTCGTTCATTTAATGGCCATTTCCGGAATGCATGTC
GGTCTGATTACCGCGGGAATATTTTATGTTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGGTTTTGCTGCTGTT
TTTTTTACCGGTGTATACGCTGTTAAGCGGTGCGGCCCCGTCCGTATTGCGCGCATCTCTCATGCTGGGATTTTATATCG
CCGGAACTCTTTTTAAACGCAGAATTCATTCCTCGGCTGCATTGTCTGTGTCTTATCTGCTGCTCCTCATGTTTAATCCT
TACCTCCTTTGGCAGGCGGGCTTCCAGCTTTCTTTTGCGGTAAGCGCCGCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGAAGATTTGCCGCGCTAGCGATGGCCTCATTCATTGCGGAGCTCAGTTCGCTTCCGTTTCTGCTCTATC
ATTTTCAGCAGATTTCGCTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCTTTTTATACGTTATTTGTCATTCCGTTT
TCGGTCATCGGTTTCCTTCTTCTCTTACTTTCAAGGCAGGTCGGGGAATGTTTGTTTTATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATGACGTATGCGGCATCCGTTGATTTATTTACAATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTGTTTACGCTATTTGCAGCTTTGGAAAAGGGCGGTTTTTTGAAACTCAGGAAATCGCCT
CTTTTTTTCTGCGCGGTTTTGGTTTATTTAATGTTCCGTCCTTATTTCAGTCCCTGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGTGCGCCGCACCGCAAAGGAACCGTAATGGTTGATACAGGGGGAGTGGTGGCTT
ATCCAGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGTGAGAAGGTTTTGATTCCGTTTTTGAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCATATCGGGGAAGCCGGAGTATTAATCAAAAA
ACATAGAGTCAAACGGCTGATTGTTCCCGTGGGATTCGTGAAGGAACCGAAGGATCAGGACATATTGAAGATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTCATCTTCAATTTCAGGTGCTGTCCCCG
GAGTCGTCTGACGGAAGAAGTAAAAACGATTCGTCACTGGTGCTTTGGACAGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAGCGTATCCGAATCTGAAGGCTGATATATTGAAGGCCGGTC
ATCACGGCAGCAAAAGCTCTACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCGGCACTGATTTCAGCAGGTGAA
GATAATCGTTACCATCATCCGCATGAAGAGGTGCTGGATCGTTTGAAAGCGTACTCTGTCAATGTGCTTCGCACCGATCT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCTGTCTTTCCTCCATATGATATAGAAGAAACCA
GGGCGCAGGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-469)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)

Domain of unknown function (DUF4131) (DUF4131)

(25-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

55.57

98.595

0.548