Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NNL22_RS12950 Genome accession   NZ_CP101527
Coordinates   2920166..2922280 (-) Length   704 a.a.
NCBI ID   WP_251811391.1    Uniprot ID   -
Organism   Alkalimarinus sediminis strain FA028     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2915166..2927280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNL22_RS12935 (NNL22_12925) - 2916552..2918255 (-) 1704 WP_251811388.1 AAA family ATPase -
  NNL22_RS12940 (NNL22_12930) aroB 2918283..2919371 (-) 1089 WP_251811389.1 3-dehydroquinate synthase -
  NNL22_RS12945 (NNL22_12935) aroK 2919453..2919989 (-) 537 WP_251811390.1 shikimate kinase AroK -
  NNL22_RS12950 (NNL22_12940) pilQ 2920166..2922280 (-) 2115 WP_251811391.1 type IV pilus secretin PilQ Machinery gene
  NNL22_RS12955 (NNL22_12945) - 2922296..2922835 (-) 540 WP_251811392.1 pilus assembly protein PilP -
  NNL22_RS12960 (NNL22_12950) - 2922832..2923452 (-) 621 WP_251811393.1 type 4a pilus biogenesis protein PilO -
  NNL22_RS12965 (NNL22_12955) - 2923449..2924012 (-) 564 WP_251811394.1 PilN domain-containing protein -
  NNL22_RS12970 (NNL22_12960) pilM 2924012..2925076 (-) 1065 WP_251811395.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 704 a.a.        Molecular weight: 75832.26 Da        Isoelectric Point: 4.7246

>NTDB_id=710408 NNL22_RS12950 WP_251811391.1 2920166..2922280(-) (pilQ) [Alkalimarinus sediminis strain FA028]
MNILYAEIDNKQLRSKNNMYYRALMLVGALCFSLFSIQASAVELKDISFASLAGDRTEVTLAFDGTPPEPKGYTIEKPAR
IALDLIGVNNGLSAKYHSLGVGNAQGLTVIGAKDRTRLIVNLVELVPYASTVDGNNIVLMIGSADAPKASTASATNATKT
AAPAASSSAKAAVTNVDFRRGDKGEGQVVLTLSSPKIDVDMSEEGGLIKLMLAGIDLPESLQRRLDVADFATPVKRIDSK
LGADGVEITIKSEGNYDYLAYQADNQFTISVEELTPQEVEQRKKDKFPYTGEKLSLNFQDIEVRSVLQLIADFTGLNLVA
SDTVGGRITLRLQNVPWDQALELVLKTKGLDKRKIGNVLLVAPADEIAAREKLELETNKQVAALAPVRLEVVQINYAKAE
DIVALLQADKELISGRGFISSDERTNTISVRETADKQEQIRRLIATWDIPVRQVLIEARIVKARTNIASDLGVQWGGAGL
ADHASHQVFGGGSFETTSQVTQGETITFPGANVVDLGVTKAGASSFALGFSNDHVLLQMELSALESDGRGEIVAQPKVVT
ADRQTATIKSGEEIPYQEASSSGATSVSFKEAVLSLEVTPQITPDGKVIMDLKINQDNRGEVTAGIPSIETNEIVTQVLV
GNGETVVLGGIFQTEESTTITKTPYLGDIPYLGTLFKRTEKVDERAELLIFITPKLLKRGLIDN

Nucleotide


Download         Length: 2115 bp        

>NTDB_id=710408 NNL22_RS12950 WP_251811391.1 2920166..2922280(-) (pilQ) [Alkalimarinus sediminis strain FA028]
ATGAATATTTTATATGCTGAAATCGATAATAAACAATTGAGGTCTAAGAATAATATGTATTATCGGGCGTTAATGCTGGT
TGGTGCACTCTGCTTTTCACTTTTTTCAATTCAGGCGAGTGCTGTCGAGTTGAAAGATATTAGTTTTGCTTCTCTGGCAG
GAGACAGAACAGAAGTTACATTGGCTTTTGATGGTACGCCGCCAGAACCGAAAGGCTACACCATAGAGAAGCCGGCTCGA
ATTGCGTTAGATTTAATCGGCGTCAATAACGGCTTGAGTGCAAAATACCACAGTCTGGGTGTGGGGAATGCGCAAGGTTT
GACAGTGATAGGCGCTAAAGATCGAACTCGCCTGATTGTAAACTTGGTAGAGCTTGTACCGTATGCGTCTACAGTGGATG
GTAATAACATCGTTCTGATGATTGGTTCTGCTGATGCTCCAAAAGCTTCTACAGCTTCTGCAACCAATGCTACAAAGACG
GCTGCTCCGGCGGCGTCTTCATCTGCTAAGGCAGCCGTGACAAATGTTGACTTTAGACGTGGTGACAAAGGTGAAGGCCA
AGTCGTGTTGACGCTTTCATCCCCTAAAATCGATGTTGATATGTCAGAAGAGGGTGGGTTGATCAAGCTAATGTTAGCGG
GGATTGATCTTCCTGAGTCGTTACAGCGTCGCTTAGATGTTGCTGACTTTGCCACTCCGGTAAAACGAATTGATTCAAAA
TTGGGTGCTGATGGCGTAGAAATAACCATCAAATCAGAAGGTAACTATGACTATCTTGCTTACCAGGCTGACAACCAATT
TACTATTAGTGTAGAAGAGTTGACCCCTCAAGAAGTTGAGCAGCGTAAGAAGGATAAGTTCCCTTACACTGGAGAAAAAC
TATCTCTTAACTTCCAAGATATCGAAGTGCGTTCGGTACTGCAGTTAATAGCAGACTTTACAGGGCTTAATCTGGTTGCG
AGTGATACTGTAGGCGGTCGAATTACACTAAGACTACAAAATGTTCCCTGGGATCAAGCGCTTGAATTAGTACTGAAAAC
CAAAGGTCTAGATAAGCGTAAGATTGGTAATGTGTTGTTAGTTGCACCAGCCGATGAAATCGCTGCTCGTGAAAAGTTAG
AGCTTGAAACCAATAAGCAGGTTGCGGCACTTGCGCCTGTTAGATTGGAAGTTGTTCAAATCAACTACGCGAAAGCTGAA
GATATAGTTGCGCTACTGCAAGCAGATAAAGAGTTGATATCAGGTCGAGGGTTTATCTCTTCTGATGAGCGTACTAACAC
TATTAGTGTGAGAGAAACGGCTGACAAGCAAGAGCAGATTAGACGTTTGATTGCGACCTGGGATATCCCTGTCCGTCAGG
TCCTTATCGAAGCGCGCATTGTTAAAGCAAGAACCAATATCGCTAGCGACCTAGGTGTACAGTGGGGTGGTGCAGGCTTG
GCGGACCATGCCAGTCATCAGGTATTTGGTGGAGGTAGCTTTGAAACCACATCTCAGGTTACCCAGGGAGAGACAATTAC
CTTCCCAGGTGCTAACGTAGTTGACCTCGGTGTGACTAAGGCGGGAGCGTCTTCGTTCGCGCTAGGCTTTAGCAACGACC
ATGTATTATTGCAGATGGAGTTATCCGCGCTTGAGTCTGATGGCCGTGGTGAAATTGTCGCTCAACCTAAGGTTGTAACG
GCAGATCGCCAAACGGCTACGATTAAGTCTGGTGAAGAAATTCCTTATCAGGAGGCATCATCAAGCGGTGCGACTTCAGT
TTCATTCAAAGAAGCCGTCTTAAGCTTAGAGGTGACGCCACAGATTACGCCTGATGGCAAAGTTATCATGGATCTCAAGA
TCAATCAGGATAATCGTGGTGAAGTGACTGCAGGTATACCAAGCATCGAGACAAACGAGATTGTTACACAGGTCTTGGTT
GGAAATGGTGAGACGGTTGTATTGGGTGGTATCTTCCAAACTGAAGAGAGTACCACTATAACCAAGACGCCTTACTTGGG
TGATATCCCATATCTGGGTACGCTGTTTAAAAGAACTGAAAAGGTTGACGAGAGAGCAGAGCTGCTTATCTTCATAACGC
CTAAATTGCTAAAGCGTGGCTTGATTGATAACTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(390-453)

AMIN domain (AMIN)

(54-136)

Bacterial type II and III secretion system protein (Secretin)

(543-697)

Secretin and TonB N terminus short domain (STN)

(317-363)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

50.85

100

0.51
  pilQ Acinetobacter baumannii strain A118

40.082

100

0.419
  pilQ Acinetobacter baumannii D1279779

40.082

100

0.419
  comQ Acinetobacter baylyi ADP1

40.839

100

0.415
  pilQ Legionella pneumophila strain Lp02

40.473

96.165

0.389
  pilQ Legionella pneumophila strain ERS1305867

40.385

96.023

0.388
  pilQ Neisseria meningitidis M1080

34.501

100

0.364