Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMB96_RS21715 Genome accession   NZ_CP101417
Coordinates   5045241..5046371 (-) Length   376 a.a.
NCBI ID   WP_006448884.1    Uniprot ID   A0A0G8P0N2
Organism   Xanthomonas hortorum strain jj2001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5040241..5051371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB96_RS21685 (NMB96_21665) - 5040659..5041093 (-) 435 WP_006448877.1 OsmC family protein -
  NMB96_RS21690 (NMB96_21670) - 5041291..5042238 (-) 948 WP_006448878.1 aspartate carbamoyltransferase catalytic subunit -
  NMB96_RS21695 (NMB96_21675) ruvX 5042235..5042723 (-) 489 WP_023904602.1 Holliday junction resolvase RuvX -
  NMB96_RS21700 (NMB96_21680) - 5042716..5043282 (-) 567 WP_006448880.1 YqgE/AlgH family protein -
  NMB96_RS21705 (NMB96_21685) - 5043580..5044125 (+) 546 WP_255085686.1 DNA-3-methyladenine glycosylase I -
  NMB96_RS21710 (NMB96_21690) - 5044263..5044937 (-) 675 WP_043889842.1 YitT family protein -
  NMB96_RS21715 (NMB96_21695) pilU 5045241..5046371 (-) 1131 WP_006448884.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMB96_RS21720 (NMB96_21700) pilT 5046478..5047515 (-) 1038 WP_006448885.1 type IV pilus twitching motility protein PilT Machinery gene
  NMB96_RS21725 (NMB96_21705) - 5048057..5048773 (+) 717 WP_255085688.1 NPP1 family protein -
  NMB96_RS21730 (NMB96_21710) - 5048930..5049622 (+) 693 WP_023904610.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMB96_RS21735 (NMB96_21715) proC 5049671..5050525 (+) 855 WP_023904611.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41991.26 Da        Isoelectric Point: 6.7684

>NTDB_id=710101 NMB96_RS21715 WP_006448884.1 5045241..5046371(-) (pilU) [Xanthomonas hortorum strain jj2001]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=710101 NMB96_RS21715 WP_006448884.1 5045241..5046371(-) (pilU) [Xanthomonas hortorum strain jj2001]
ATGAGCACTATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTCAACGGCAAGATTAGCCCGATCACCCAGACACCGCTGACGGCGCAACAAAGTCGCGATTTGG
TGTTGAACGTGATGACACCGTCGCAGCGCGAAGAATTCGAAAAGACGCACGAGTGCAACTTCGCCATCGGCGTGTCAGGG
GTCGGGCGTTTTCGTGTGAGCTGTTTCTATCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACGGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATTTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGACACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGTGAAGTCGGCATCGACACCGATAG
TTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAAGGCA
TGGATCACGCGGTGGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAATGCCAACCAGGCGATG
GACCGCATCATCAACTTTTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAATTGATTCCGACTCCGGACGGGCGCAGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACCCCGCTGGTGC
AGGATTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGTACCTTC
GATCAGAGCCTGTTCGAGCTGTATCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGCTATGCCGATTCGCAGAACGA
AGTGCGCCTGCGCATCAAACTCTCGCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGGATGGATGGTGTGGAGATTGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8P0N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.667

98.138

0.654
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364