Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J58_RS03605 Genome accession   NZ_AP018772
Coordinates   709669..710118 (+) Length   149 a.a.
NCBI ID   WP_005686726.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-1 isolate 15H02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 704669..715118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J58_RS03585 (CHBNIII1_06940) rsmE 705135..705872 (-) 738 WP_005662982.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J58_RS03590 (CHBNIII1_06950) lnt 705922..707451 (-) 1530 WP_105887052.1 apolipoprotein N-acyltransferase -
  K6J58_RS03595 (CHBNIII1_06960) corC 707474..708373 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J58_RS03600 (CHBNIII1_06970) ampD 708994..709554 (-) 561 WP_005686728.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J58_RS03605 (CHBNIII1_06980) pilA 709669..710118 (+) 450 WP_005686726.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J58_RS03610 (CHBNIII1_06990) pilB 710115..711512 (+) 1398 WP_005686724.1 GspE/PulE family protein Machinery gene
  K6J58_RS03615 (CHBNIII1_07000) pilC 711509..712726 (+) 1218 WP_005686722.1 type II secretion system F family protein Machinery gene
  K6J58_RS03620 (CHBNIII1_07010) pilD 712723..713415 (+) 693 WP_105887051.1 A24 family peptidase Machinery gene
  K6J58_RS03625 (CHBNIII1_07020) rho 713469..714731 (-) 1263 WP_005648966.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15542.90 Da        Isoelectric Point: 8.4607

>NTDB_id=71006 K6J58_RS03605 WP_005686726.1 709669..710118(+) (pilA) [Haemophilus influenzae strain CHBN-III-1 isolate 15H02]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNEATKCTGGKNGIA
ADITTAKGYVKSVTTSNGAITVKGDGTLANMEYILQASGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=71006 K6J58_RS03605 WP_005686726.1 709669..710118(+) (pilA) [Haemophilus influenzae strain CHBN-III-1 isolate 15H02]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATCCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAGCAACAAAGTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCCAGTGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

97.987

100

0.98
  pilA Haemophilus influenzae Rd KW20

91.275

100

0.913
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila strain ERS1305867

42.446

93.289

0.396
  pilA2 Legionella pneumophila str. Paris

42.446

93.289

0.396
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376

Multiple sequence alignment