Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NLG07_RS05775 Genome accession   NZ_CP101291
Coordinates   1233799..1234191 (-) Length   130 a.a.
NCBI ID   WP_254856748.1    Uniprot ID   -
Organism   Alteromonas sp. LMIT006     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1228799..1239191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLG07_RS05745 (NLG07_05745) - 1229022..1229894 (-) 873 WP_254856741.1 aspartoacylase -
  NLG07_RS05750 (NLG07_05750) yacG 1229900..1230130 (-) 231 WP_254856743.1 DNA gyrase inhibitor YacG -
  NLG07_RS05755 (NLG07_05755) zapD 1230130..1230882 (-) 753 WP_254856744.1 cell division protein ZapD -
  NLG07_RS05760 (NLG07_05760) coaE 1230922..1231536 (-) 615 WP_254856745.1 dephospho-CoA kinase -
  NLG07_RS05765 (NLG07_05765) pilD 1231536..1232459 (-) 924 WP_254856746.1 A24 family peptidase Machinery gene
  NLG07_RS05770 (NLG07_05770) pilC 1232499..1233710 (-) 1212 WP_254856747.1 type II secretion system F family protein Machinery gene
  NLG07_RS05775 (NLG07_05775) pilA 1233799..1234191 (-) 393 WP_254856748.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NLG07_RS05780 (NLG07_05780) nadC 1234366..1235283 (-) 918 WP_254856749.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NLG07_RS05785 (NLG07_05785) - 1235280..1235858 (-) 579 WP_254856750.1 TIGR02281 family clan AA aspartic protease -
  NLG07_RS05790 (NLG07_05790) ampD 1235889..1236458 (+) 570 WP_254856751.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NLG07_RS05795 (NLG07_05795) ampE 1236455..1237279 (+) 825 WP_254856752.1 regulatory signaling modulator protein AmpE -
  NLG07_RS05800 (NLG07_05800) - 1237488..1238243 (+) 756 WP_254856753.1 GntR family transcriptional regulator -

Sequence

Protein


Download         Length: 130 a.a.        Molecular weight: 13271.16 Da        Isoelectric Point: 4.7330

>NTDB_id=709523 NLG07_RS05775 WP_254856748.1 1233799..1234191(-) (pilA) [Alteromonas sp. LMIT006]
MNTLFTSKNNLGFTLIELMIVVAIIGILAAIALPAYSNYTNKAEFTEVTQATAAAKTAVGVCVQVTGATTNCNGSSNGIP
DNITTGDVTVTTTAGVILATHDLGTYTLTPTVATNGRVTWGAVCNPATLC

Nucleotide


Download         Length: 393 bp        

>NTDB_id=709523 NLG07_RS05775 WP_254856748.1 1233799..1234191(-) (pilA) [Alteromonas sp. LMIT006]
ATGAACACTTTATTCACTAGCAAAAACAACCTGGGTTTTACCTTAATTGAATTAATGATTGTGGTCGCAATTATCGGCAT
ACTCGCCGCTATTGCACTGCCTGCCTATAGTAACTACACGAACAAAGCTGAATTTACCGAGGTCACTCAGGCTACAGCTG
CGGCCAAGACAGCCGTCGGAGTCTGTGTCCAAGTAACTGGAGCAACAACAAATTGCAACGGTTCATCTAACGGGATTCCA
GATAATATCACTACTGGTGATGTAACCGTCACTACAACAGCTGGTGTCATTCTTGCTACCCATGATTTAGGTACGTACAC
ATTGACTCCAACTGTTGCAACTAATGGTCGTGTTACTTGGGGAGCCGTTTGTAACCCTGCAACCTTATGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

47.015

100

0.485
  pilA/pilA1 Eikenella corrodens VA1

44.526

100

0.469
  pilA Vibrio campbellii strain DS40M4

42.553

100

0.462
  pilA Haemophilus influenzae Rd KW20

43.284

100

0.446
  comP Acinetobacter baylyi ADP1

45.238

96.923

0.438
  pilA Glaesserella parasuis strain SC1401

37.5

100

0.392
  pilA2 Legionella pneumophila str. Paris

42.735

90

0.385
  pilA2 Legionella pneumophila strain ERS1305867

42.735

90

0.385