Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NGC85_RS16865 Genome accession   NZ_CP101115
Coordinates   3553719..3554894 (+) Length   391 a.a.
NCBI ID   WP_254804434.1    Uniprot ID   -
Organism   Acinetobacter sp. Z1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3548719..3559894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGC85_RS16840 (NGC85_16840) - 3549284..3549838 (-) 555 WP_254804431.1 hypothetical protein -
  NGC85_RS16845 (NGC85_16845) guaD 3550037..3551353 (+) 1317 WP_254804432.1 guanine deaminase -
  NGC85_RS16850 (NGC85_16850) hpt 3551579..3552106 (+) 528 WP_005055385.1 hypoxanthine phosphoribosyltransferase -
  NGC85_RS16855 (NGC85_16855) - 3552275..3552526 (+) 252 WP_005055384.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  NGC85_RS16860 (NGC85_16860) - 3552576..3553274 (-) 699 WP_254804433.1 DsbC family protein -
  NGC85_RS16865 (NGC85_16865) pilU 3553719..3554894 (+) 1176 WP_254804434.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NGC85_RS16870 (NGC85_16870) - 3554944..3555756 (-) 813 WP_254804435.1 DUF2310 family Zn-ribbon-containing protein -
  NGC85_RS16875 (NGC85_16875) mnmE 3555917..3557272 (-) 1356 WP_254804436.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  NGC85_RS16880 (NGC85_16880) yidC 3557386..3559143 (-) 1758 WP_005055378.1 membrane protein insertase YidC -
  NGC85_RS16885 (NGC85_16885) yidD 3559149..3559469 (-) 321 WP_169109820.1 membrane protein insertion efficiency factor YidD -
  NGC85_RS16890 (NGC85_16890) rnpA 3559479..3559874 (-) 396 WP_254804437.1 ribonuclease P protein component -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44248.73 Da        Isoelectric Point: 6.3233

>NTDB_id=708759 NGC85_RS16865 WP_254804434.1 3553719..3554894(+) (pilU) [Acinetobacter sp. Z1]
MYNAELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQELTAEKTKLFAYSLMNEKQRQDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTEDGKGRRAAIEILLNTPLIGDNILKGQFHVLKEIMSKS
RELGMQTFDQALFGLYNEGAISYDEALRNADSVNELRLQIKLKSTRQEGMPVNSAASLDMVKEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=708759 NGC85_RS16865 WP_254804434.1 3553719..3554894(+) (pilU) [Acinetobacter sp. Z1]
ATGTATAACGCGGAACTATTAGAAGAAGCAAAAACCTTTATGTTTCACATGCTCACCAAAGTAGTTGAATATGGTGGCTC
AGATTTATTTATTACAGCAGATTTTCCACCGAGTATTAAATTACAAGGCTTGATGCGACCGTTAGGACAACAAGAATTAA
CTGCGGAAAAAACCAAGTTATTTGCTTATAGTTTAATGAACGAAAAACAACGACAAGACTTTGAAAATGATTGGGAATGT
AATTTTGCCATCAATGTACCCAATGTATCGCGCTTTCGCGTCAATGTATTCAAACAGCAGCTGCAAATTGGTATGGTCAT
TCGTACCATTACATCAGAAATTCCAACCTTTCAAAAATTAAAACTTCCTGAATCGCTTAAAAATGTGATTATGGAAAAAC
GTGGTTTAGTCCTCGTGGTGGGTGGAACTGGTTCAGGTAAATCGACATCATTAGCAGCAATGATTGATCATCGTAATGAA
AATTCAGCAGGGCATATTATTACGGTTGAAGATCCAGTTGAATACGTACATAAACATAAGAAATCGATGATTACCCATCG
AGAAGTCGGTGTCGATTGTCACTCATGGCATCACGCACTCAAAAACACCCTGCGCCAAGCACCTGATGTGATTTTAATTG
GTGAAATCCGTGATACAGAAACTATGGAGCATGCGATTGCTTTTGCTGAAACAGGACATCTCTGTTTAGGGACTTTACAC
GCAAATAATGCCAACCAGGCCTTAGATCGTATTATTAACTTTTTCCCAGAAGAACGTCGCAATCAATTATTGATGGATCT
TTCATCCAATATGAAAGCGATTATTTCACAACGTCTGGTGCGCACTGAAGATGGTAAAGGGCGTCGAGCTGCGATTGAAA
TTTTACTGAATACGCCGTTGATTGGTGACAATATTCTAAAAGGACAATTCCATGTTCTTAAAGAAATCATGAGCAAGTCA
CGTGAGTTAGGGATGCAAACCTTCGATCAAGCCTTATTTGGGCTTTATAATGAAGGAGCTATTAGCTACGATGAAGCACT
GCGTAACGCTGATTCTGTCAATGAATTACGTTTACAAATTAAACTTAAAAGTACGCGCCAAGAAGGAATGCCTGTCAATT
CTGCGGCTTCATTGGATATGGTGAAAGAAGAAGTTGAAGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(103-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.632

89.003

0.54
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

50.949

94.373

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.114

85.422

0.368
  pilT Acinetobacter nosocomialis M2

43.636

84.399

0.368
  pilT Acinetobacter baumannii D1279779

43.865

83.376

0.366
  pilT Acinetobacter baumannii strain A118

43.865

83.376

0.366
  pilT Acinetobacter baylyi ADP1

42.262

85.934

0.363